1.Trends in genotype frequency resulting from breeding for resistance to classical scrapie in Belgium (2006~2011).
Alexandre DOBLY ; Sara VAN DER HEYDEN ; Stefan ROELS
Journal of Veterinary Science 2013;14(1):45-51
In sheep, susceptibility to scrapie is mainly determined by codons 136, 154, and 171 of the PRNP gene. Five haplotypes are usually present (ARR, ARQ, ARH, AHQ, and VRQ). The ARR haplotype confers the greatest resistance to classical scrapie while VRQ renders animals most susceptible. In 2004, the European Union implemented a breeding program that promotes selection of the ARR haplotype while reducing the incidence of VRQ. From 2006 to 2011 in Belgium, frequency for the ARR/ARR genotypes increased from 38.3% to 63.8% (n = 6,437), the ARQ haplotype diminished from 21.1% to 12.9%, and the VRQ haplotype decreased from 2.0% to 1.7%. The status of codon 141, a determinant for atypical scrapie, was also evaluated. Out of 27 different breeds (n = 5,163), nine were abundant. The ARR/ARR frequency increased in eight of these nine major breeds. The selection program has had a major impact on the ARR haplotype frequency in Belgium. However, the occurrence of atypical scrapie represents a critical point for this program that warrants the continuous monitoring of scrapie. Additionally, genotype frequencies among the breeds varied greatly. Texel, a breed that is common in Belgium, can still be selected for due to its average ARR frequency.
Animals
;
Belgium
;
*Breeding
;
Female
;
*Genetic Predisposition to Disease
;
Genetic Variation
;
*Genotype
;
Male
;
Scrapie/*genetics
;
Sheep
2.Analysis of monoclonal antibody binding sites in ovine prion protein.
Yongqiang ZHANG ; Xiaodong WU ; Yonggang ZHAO ; Endong BAO ; Qinghua WANG ; Wei ZHANG ; Yutian LIU ; Zhiliang WANG
Chinese Journal of Biotechnology 2009;25(3):348-353
Binding sites of five monoclonal antibodies were obtained by reinforceable method of overlapping recombinant prion protein and synthetic peptide. Overlapping peptides of PrP core were expressed in Escherichia coli by insertion of serial PCR amplicons of ovine PrP gene fragments into pET32a. The expressed fusion peptides were then tested for the binding activity to PrP monoclonal antibodies in Western blotting. The binding sites of 5 monoclonal antibodies of ovine PrP were located respectively as follows: 2H3 in 199 aa-213 aa, 4C6, 5F11 and 7F11 in 139 aa-168 aa and 7F1 in 214 aa-227 aa. There oligo peptides were synthesized and used in ELISA test for more accurate localization of the binding sites. The binding sites of 4C6, 5F11 and 7F11 were further confirmed to be in 149 aa-158 aa. This conclusion may contribute to the research for pathogenesis and diagnostic method of scrapie and bovine transmissible spongiform encephalopathy.
Animals
;
Antibodies, Monoclonal
;
immunology
;
metabolism
;
Binding Sites, Antibody
;
immunology
;
Epitopes
;
immunology
;
Escherichia coli
;
genetics
;
metabolism
;
Prion Diseases
;
diagnosis
;
Prions
;
genetics
;
immunology
;
metabolism
;
Recombinant Fusion Proteins
;
genetics
;
immunology
;
metabolism
;
Scrapie
;
diagnosis
;
Sheep