1.Toxicity of crude and detoxified Tityus serrulatus venom in anti-venom-producing sheep.
Marina G FERREIRA ; Clara G DUARTE ; Maira S OLIVEIRA ; Karen L P CASTRO ; Maílson S TEIXEIRA ; Lílian P G REIS ; José A ZAMBRANO ; Evanguedes KALAPOTHAKIS ; Ana Flávia R M MICHEL ; Benito SOTO-BLANCO ; Carlos CHÁVEZ-OLÓRTEGUI ; Marília M MELO
Journal of Veterinary Science 2016;17(4):467-477
Specific anti-venom used to treat scorpion envenomation is usually obtained from horses after hyperimmunization with crude scorpion venom. However, immunized animals often become ill because of the toxic effects of the immunogens used. This study was conducted to evaluate the toxic and immunogenic activities of crude and detoxified Tityus serrulatus (Ts) venom in sheep during the production of anti-scorpionic anti-venom. Sheep were categorized into three groups: G1, control, immunized with buffer only; G2, immunized with crude Ts venom; and G3, immunized with glutaraldehyde-detoxified Ts venom. All animals were subjected to clinical exams and supplementary tests. G2 sheep showed mild clinical changes, but the other groups tolerated the immunization program well. Specific antibodies generated in animals immunized with either Ts crude venom or glutaraldehyde-detoxified Ts venom recognized the crude Ts venom in both assays. To evaluate the lethality neutralization potential of the produced sera, individual serum samples were pre-incubated with Ts crude venom, then subcutaneously injected into mice. Efficient immune protection of 56.3% and 43.8% against Ts crude venom was observed in G2 and G3, respectively. Overall, the results of this study support the use of sheep and glutaraldehyde-detoxified Ts venom for alternative production of specific anti-venom.
Animals
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Antibodies
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Horses
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Immunization Programs
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Mice
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Scorpion Venoms
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Scorpions
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Sheep*
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Venoms*
2.The expression of BmK AngM1 in Mut(s) and Mut(+) recombinants of Pichia pastoris.
Qing-hua WANG ; Lan LIANG ; Jing-jing CHEN ; Ting GONG ; Qi HOU ; Jin-ling YANG ; Ping ZHU
Acta Pharmaceutica Sinica 2015;50(7):910-915
BmK AngM1 is a long-chain scorpion toxin purified from the venom of Buthus martensii Karsch. It has been reported to exhibit evident analgesic effect and low toxicity, and has the potential to be a novel analgesic drug. The BmKAngM1 gene was transformed into Pichiapastoris GS115. Mut+ and Mut(s) recombinant strains were screened by phenotype and Mut+ recombinant strains were used to detect BmK AngMl gene copy number in the real-time PCR. Expression of BmK AngM1 in the Mut+ recombinant strain was compared with that of the Mut(s) recombinant strain with the same single copy of BmK AngM1 gene under the same condition. The results indicated that the transcription level of BmK AngM1 gene in the Mut(s) recombinant strain was 2.7 fold of that in the Mut recombinant strain in the real-time PCR, and the expression of BmK AngM 1 in the Mut(s) recombinant strain was 1.5 fold of that in the Mut+ recombinant strain. Therefore, Mut(s) recombinant strain showed better ability to express BmK AngM1 than Mut+ recombinant strain.
Animals
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Arthropod Proteins
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biosynthesis
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Gene Dosage
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Pichia
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metabolism
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Recombinant Proteins
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biosynthesis
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Scorpion Venoms
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chemistry
3.The protection of scorpion venom derived activity peptide against the change of dynorphin in the early Parkinson's disease rats.
Dong-mei WANG ; Dan ZHAO ; Sheng-ming YIN ; Dong AN ; Wei CHEN ; De-qin YU ; Hong XU ; Jie ZHAO ; Wan-qin ZHANG ; Yu-xiang TIAN
Chinese Journal of Applied Physiology 2015;31(2):120-122
Animals
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Dynorphins
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metabolism
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Parkinson Disease
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drug therapy
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metabolism
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Peptides
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pharmacology
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Rats
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Scorpion Venoms
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pharmacology
4.AcMNPV-mediated expression of BmK IT promotes the apoptosis of Sf9 cells and replication of AcMNPV.
Yue-Jun FU ; Jie ZHAO ; Ai-Hua LIANG ; Feng-Yun HU
Acta Physiologica Sinica 2015;67(3):305-311
Chinese scorpion Buthus martensii Karsch (BmK) venom is a rich source of neurotoxins which bind to various ion channels with high affinity and specificity and thus widely used as compounds to modulate channel gating or channel currents. To promote the insecticidal effects of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV), the gene encoding an excitatory insect toxin, BmK IT, was inserted into the genome of AcMNPV to construct a recombinant baculovirus, AcMNPV-BmK IT. Spodopter frugiperda 9 (Sf9) cells were infected with AcMNPV and AcMNPV-BmK IT respectively for 24 h. Results from the MTT assay, TUNEL assay, analysis of the expression level of apoptosis-related proteins (c-Myc, cleaved-Caspase3, Bcl-2 and Bax) of Sf9 cells, the transcription level of key genes (38K, C42, P78, F) of AcMNPV, and viral propagation assay demonstrated that AcMNPV-mediated expression of BmK IT promoted the apoptosis of Sf9 cells and replication of AcMNPV. The results laid a foundation for further structural and functional analysis of BmK IT.
Animals
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Apoptosis
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Cell Line
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Nucleopolyhedrovirus
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metabolism
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physiology
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Scorpion Venoms
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biosynthesis
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Sf9 Cells
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drug effects
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Virus Replication
5.Unique interactions between scorpion toxins and small conductance Ca(2+)-activated potassium channels.
Fan YANG ; Zong-Yun CHEN ; Ying-Liang WU
Acta Physiologica Sinica 2015;67(3):255-260
Small conductance Ca(2+)-activated potassium channels (SK channels) distributing in the nervous system play an important role in learning, memory and synaptic plasticity. Most pharmacological properties of them are determined by short-chain scorpion toxins. Different from most voltage-gated potassium channels and large-conductance Ca(2+)-activated potassium channels, SK channels are only inhibited by a small quantity of scorpion toxins. Recently, a novel peptide screener in the extracellular pore entryway of SK channels was considered as the structural basis of toxin selective recognition. In this review, we summarized the unique interactions between scorpion toxins and SK channels, which is crucial not only in deep-researching for physiological function of SK channels, but also in developing drugs for SK channel-related diseases.
Animals
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Memory
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Neuronal Plasticity
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Scorpion Venoms
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chemistry
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Scorpions
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Small-Conductance Calcium-Activated Potassium Channels
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antagonists & inhibitors
6.Shal-type (Kv4.x) potassium channel pore blockers from scorpion venoms.
Pierre Edouard BOUGIS ; Marie-France MARTIN-EAUCLAIRE
Acta Physiologica Sinica 2015;67(3):248-254
Voltage-gated potassium channels (Kv4.1, Kv4.2 and Kv4.3) encoded by the members of the KCND/Kv4 (Shal) channel family mediate the native, fast inactivating (A-type) K(+) current (IA) described both in heart and neurons. This IA current is specifically blocked by short scorpion toxins that belong to the α-KTx15 subfamily and which act as pore blockers, a different mode of action by comparison to spider toxins known as gating modifiers. This review summarizes our present chemical and pharmacological knowledge on the α-KTx15 toxins.
Animals
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Potassium Channel Blockers
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chemistry
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Scorpion Venoms
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chemistry
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Scorpions
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Shal Potassium Channels
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antagonists & inhibitors
7.Bioactive peptides from scorpion venoms: therapeutic scaffolds and pharmacological tools.
Kamau PETER MUIRURI ; Jian ZHONG ; Bing YAO ; Ren LAI ; Lei LUO
Chinese Journal of Natural Medicines (English Ed.) 2023;21(1):19-35
Evolution and natural selection have endowed animal venoms, including scorpion venoms, with a wide range of pharmacological properties. Consequently, scorpions, their venoms, and/or their body parts have been used since time immemorial in traditional medicines, especially in Africa and Asia. With respect to their pharmacological potential, bioactive peptides from scorpion venoms have become an important source of scientific research. With the rapid increase in the characterization of various components from scorpion venoms, a large number of peptides are identified with an aim of combating a myriad of emerging global health problems. Moreover, some scorpion venom-derived peptides have been established as potential scaffolds helpful for drug development. In this review, we summarize the promising scorpion venoms-derived peptides as drug candidates. Accordingly, we highlight the data and knowledge needed for continuous characterization and development of additional natural peptides from scorpion venoms, as potential drugs that can treat related diseases.
Animals
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Scorpion Venoms/pharmacology*
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Peptides/pharmacology*
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Scorpions
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Drug Development
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Medicine, Traditional
8.Anti-epileptic/pro-epileptic effects of sodium channel modulators from Buthus martensii Karsch.
Qian XIAO ; Zhi-Ping ZHANG ; Yang-Bo HOU ; Dong-Xiao QU ; Le-Le TANG ; Li-Ji CHEN ; Guo-Yi LI ; Yong-Hua JI ; Jie TAO ; Yu-Dan ZHU
Acta Physiologica Sinica 2022;74(4):621-632
The East Asian scorpion Buthus martensii Karsch (BmK) is one of the classical traditional Chinese medicines for treating epilepsy for over a thousand years. Neurotoxins purified from BmK venom are considered as the main active ingredients, acting on membrane ion channels. Voltage-gated sodium channels (VGSCs) play a crucial role in the occurrence of epilepsy, which make them become important drug targets for epilepsy. Long chain toxins of BmK, composed of 60-70 amino acid residues, could specifically recognize VGSCs. Among them, α-like neurotoxins, binding to the receptor site-3 of VGSC, induce epilepsy in rodents and can be used to establish seizure models. The β or β-like neurotoxins, binding to the receptor site-4 of VGSC, have significant anticonvulsant effects in epileptic models. This review aims to illuminate the anticonvulsant/convulsant effects of BmK polypeptides by acting on VGSCs, and provide potential frameworks for the anti-epileptic drug-design.
Animals
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Anticonvulsants/therapeutic use*
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Neurotoxins/pharmacology*
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Scorpion Venoms/pharmacology*
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Scorpions/chemistry*
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Voltage-Gated Sodium Channels
9.Anti-proliferative Effects of Androctonus amoreuxi Scorpion and Cerastes cerastes Snake Venoms on Human Prostate Cancer Cells.
Hassan AKEF ; Nahla KOTB ; Dina ABO-ELMATTY ; Sayed SALEM
Journal of Cancer Prevention 2017;22(1):40-46
The present study evaluated the effects of Androctonus amoreuxi scorpion venom, Cerastes cerastes snake venom and their mixture on prostate cancer cells (PC3). An MTT assay was used to determine the anti-proliferative effect of the venoms, while quantitative real time PCR was used to evaluate the expression of apoptosis-related genes (Bax and Bcl-2). Furthermore, colorimetric assays were used to measure the levels of malondialdehyde (MDA) and antioxidant enzymes. Our results show that the venoms significantly reduced PC3 cell viability in a dose-dependent manner. On the other hand, these venoms significantly decreased Bcl-2 gene expression. Additionally, C. cerastes venom significantly reduced Bax gene expression, while A. amoreuxi venom and a mixture of A. amoreuxi & C. cerastes venoms did not alter Bax expression. Consequently, these venoms significantly increased the Bax/Bcl-2 ratio and the oxidative stress biomarker MDA. Furthermore, these venoms also increased the activity levels of the antioxidant enzymes, catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase, and glutathione-S-transferase. Overall, the venoms have cytotoxic and anti-proliferative effects on PC3 cells.
Apoptosis
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Catalase
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Cell Survival
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Gene Expression
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Genes, bcl-2
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Glutathione Peroxidase
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Glutathione Reductase
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Hand
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Humans*
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Malondialdehyde
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Oxidative Stress
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Prostate*
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Prostatic Neoplasms*
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Real-Time Polymerase Chain Reaction
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Scorpion Venoms
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Scorpions*
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Snake Venoms*
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Snakes*
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Superoxide Dismutase
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Venoms
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Viper Venoms
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Viperidae*
10.Effects of BmkTXK beta on electrophysiological properties of rabbit atrial myocytes.
Dan HU ; Congxin HUANG ; Hong JIANG ; Gengshan LI ; Zhijian CAO ; Wenxin LI ; Shimin WANG
Chinese Medical Journal 2003;116(11):1691-1696
OBJECTIVETo investigate the effects of BmkTXK(beta), a newly purified 'long chain' peptide inhibitor of K(+) channels from the Chinese scorpion Buthus martensi Karsch (BmK), on the electrophysiological properties of isolated rabbit atrial myocytes.
METHODSThe standard whole-cell patch-clamp technique was used to study the effects of multiple concentrations of BmkTXK(beta) on potassium currents and action potentials.
RESULTSBmkTXK(beta) produced concentration-dependent prolongation of action potential duration at 20%, 50%, and 90% repolarization (APD(20,50,90)) without any use-dependence. Meanwhile, it had no significant effect on RMP, APA, or V(max) (n = 9). At a dose of 1 micro mol/L, BmkTXK(beta) decreased I(to) by 41.4% (n = 10, P < 0.01) at a membrane potential of +50 mV [from (13.63 +/- 0.87) pA/pF to (7.98 +/- 0.78) pA/pF]. I(to) was reduced significantly with an IC(50) value of 1.82 micromol/L (95% confidence interval: 1.47 - 2.17 micro mol/L), in a clear concentration-dependent manner. BmkTXK(beta) blocked I(Ks) and I(Ks),tail with an IC(50) of 20.15 micromol/L and a 95% confidence interval of 16.93 - 23.37 micromol/L. At a concentration of 10 micromol/L, BmkTXK(beta) blocked both I(Ks) (mean reduction 37.3% +/- 4.2%, P < 0.01, n = 7) and I(Ks), tail (mean reduction 35.8% +/- 4.1%, P < 0.01, n = 7). At 0 mV, 10 micromol/L BmkTXK(beta) inhibited both I(Kr) (mean reduction 40.5% +/- 2.6%, P < 0.01, n = 6) and I(Kr), tail (mean reduction 42.3% +/- 2.9%, P < 0.01, n = 6). Blocking of I(Kr) by BmkTXK(beta) occurred in a concentration-dependent manner, with an IC(50) of 17.21 micromol/L (95% confidence interval: 14.76-19.66 micromol/L). An absence of effects on I(K1) was observed for BmkTXK(beta), with no change in reversal-potential (n = 6, P > 0.05).
CONCLUSIONSBmkTXK(beta) exerts direct blocking effects on several potassium channels involved in cardiac repolarization, and has a strong effect on prolonging the repolarization of rabbit cardiomyocytes without reverse frequency dependence. This finding suggests that BmkTXK(beta) could be a promising class III drug for anti-arrhythmic therapy without the risk of proarrhythmia.
Action Potentials ; drug effects ; Animals ; Cells, Cultured ; Heart Atria ; Myocardium ; cytology ; Patch-Clamp Techniques ; Rabbits ; Scorpion Venoms ; pharmacology