From total dried extract extracted by methanol from roots of Nho dong (Morinda longisima Y.Z.Ruan, Rubiaceae) that was gathered in Chieng An commune, Son La town in Apr 2002, a compound called CP2 was isolated through chromatography. Spectral analyses showed CP2 was a coumarin named as Scopoletin. This was the first time Scopoletin found in root of Nho dong
Scopoletin ; Plants, medicinal ; Medicine, Traditional

Fourteen compounds were isolated from the stem of Angelica polymorpha. On the basis of spectral data, these compounds were identified as isoimperatorin (1), phellopterin (2), bergapten (3), xanthyletin (4), cnidilin (5), geijerine (6), (−)-3'-acetyl hamaudol (7), 7-demethylsuberosine (8), dehydrogeijerin (9), (−)-hamaudol (10), (+)-visamminol (11), divaricatol (12), scopoletin (13), and decursidate (14), respectively. Among them, compounds 4 - 6, 8, 9, 13, and 14 were isolated for the first time from A. polymorpha. Dehydrogeijerin (6) and geijerin (9) were isolated for the first time from genus Angelica. All isolates tested for inhibitory activity against acetylcholinesterae. Compounds 1 to 13 showed acetylcholinesterase inhibitory activity with IC₅₀ values ranging from 1.4 to 37.5 µM.
Acetylcholinesterase* ; Angelica* ; Cholinesterase Inhibitors* ; Chromones ; Coumarins ; Scopoletin

Scopoletin is a coumarin compound with various biological activities including detumescence and analgesic, insecticidal, antibacterial and acaricidal effects. However, interference with scopolin and other components often leads to difficulties in purification of scopoletin with low extraction rates from plant resource. In this paper, heterologous expression of the gene encoding β-glucosidase An-bgl3 derived from Aspergillus niger were carried out. The expression product was purified and characterized with further structure-activity relationship between it and β-glucosidase analyzed. Subsequently, its ability for transforming scopolin from plant extract was studied. The results showed that the specific activity of the purified β-glucosidase An-bgl3 was 15.22 IU/mg, the apparent molecular weight was about 120 kDa. The optimum reaction temperature and pH were 55 ℃ and 4.0, respectively. Moreover, 10 mmol/L metal ions Fe²⁺ and Mn²⁺ increased the enzyme activity by 1.74-fold and 1.20-fold, respectively. A 10 mmol/L solution containing Tween-20, Tween-80 and Triton X-100 all inhibited the enzyme activity by 30%. The enzyme showed affinity towards scopolin and tolerated 10% methanol and 10% ethanol solution, respectively. The enzyme specifically hydrolyzed scopolin into scopoletin from the extract of Erycibe obtusifolia Benth with a 47.8% increase of scopoletin. This demonstrated that the β-glucosidase An-bgl3 from A. niger shows specificity on scopolin with good activities, thus providing an alternative method for increasing the extraction efficiency of scopoletin from plant material.
Aspergillus niger/genetics* ; beta-Glucosidase/chemistry* ; Scopoletin ; Polysorbates ; Coumarins

The stem barks, heartwoods, and leaves of Acer tegmentosum (Aceraceae) are widely used in Korea to treat hepatic or cerebral disorders mainly due to alcohol poisoning. This study was aimed to analyze phenolic substances in A. tegmentosum. Quantitative analysis of the three phenolic substances (salidroside, (+)-catechin and scopoletin) was performed by HPLC and the identification of volatile phenolic substances were done by GC-MS. The contents of the three compounds in the three MeOH extracts were higher in the stem bark (salidroside: 80.22 mg/g, (+)-catechin: 23.31 mg/g, and scopoletin: 9.45 mg/g) compared to the heartwoods and leaves. And GC-MS analysis of the stem bark extract demonstrated that p-tyrosol is a main substance of twenty-one compounds identified.
Acer* ; Aceraceae ; Catechin ; Chromatography, High Pressure Liquid* ; Korea ; Phenol* ; Poisoning ; Scopoletin

OBJECTIVETo study the chemical constituents of Phellinus igniarius.

METHODCompounds were isolated by nomal phasc sillica gel and Al2O3 chromatography and reverse phase HPLC. Their structures were elucidated by spectroscopic methods including IR, MS and NMR.

RESULTFive flavonoids and two coumarins were isolated from Phellinus igniarius and their structures were identified as naringenin, sakuranetin, aromadendrin, folerogenin, eriodictyol, coumarin and scopoletin.

CONCLUSIONAll these compounds were obtained from this genus for the first time.

Flavanones ; chemistry ; isolation & purification ; Flavonoids ; chemistry ; isolation & purification ; Polyporaceae ; chemistry ; Scopoletin ; chemistry ; isolation & purification

Twelve compounds were separated from stems of Dysoxylum laxiracemosum and their structures were identified by spectrum analysis as shoreic acid (1), cabraleahydroxylactone (2), cabralealactone (3), cinchonain (5), catechin (6), scopoletin (7), vanillic acid (8), p-hydroxybenzoic acid (9), docosanol (10), beta-sitosterol (11), daucosterol (12). Of them, compounds 1-6,8-12 were separated from this plant for the first time, and compounds 4-6 were reported from this plant genus for the first time.
Catechin ; chemistry ; Meliaceae ; chemistry ; Plant Stems ; chemistry ; Scopoletin ; chemistry ; Sitosterols ; chemistry ; Vanillic Acid ; chemistry

OBJECTIVETo study the chemical constituents of the twigs of Morus atropurpurea.

METHODThe compounds of the EtOAc fraction were isolated and purified by column chromatography on silica gel, polyamide, Sephadex LH -20, and their structures were elucidated on the basis of spectroscopic evidence (MS, NMR).

RESULTEleven compounds were identified as mulberrin (1), cyclomulberrin (2), morusin (3), cyclomorusin (4), 2', 4',4, 2"-tetrahydroxy-3'-{3"-methylbut-3"-enyl-}-chalcone (5), mulberrofran G (6), scopoletin (7), moruchalcone A (8), kaempferol (9), ursolic acid (10), beta-daucosterol (11).

CONCLUSIONExcept compounds 9 and 11, all the other compounds were obtained from M. atropurpurea for the first time.

Flavonoids ; chemistry ; Kaempferols ; chemistry ; Magnetic Resonance Spectroscopy ; Morus ; chemistry ; Phytosterols ; chemistry ; Plant Stems ; chemistry ; Scopoletin ; chemistry ; Triterpenes ; chemistry

OBJECTIVETo study the chemical constituents of Suregada glomeruldata.

METHODIsolation and purification were carried out on silica gel, sephardex LH -20, ODS column chromatography etc. Constituents were identified by physicochemical properties and spectral analysis.

RESULTNine compounds, Jolkinolide B (1), 8alpha, 14-dihydro-7-oxo-jolkinolide E (2), helioscopinolide B (3), ent-kauran-16beta, 17-diol (4), 7-oxo-beta-sitosterol (5), scopoletin (6), adenosine (7), 1'-sinapoylglucose (8), sucrose (9), were obtained and identified.

CONCLUSIONCompounds 2-9 were isolated from S. glomerulata for the first time.

Chromatography, Gel ; Diterpenes, Abietane ; chemistry ; isolation & purification ; Plants, Medicinal ; chemistry ; Scopoletin ; chemistry ; isolation & purification ; Sucrose ; chemistry ; isolation & purification ; Suregada ; chemistry

The objective of this study is to develop a sensitive and reliable high-performance liquid chromatography mass spectrometry (LC-MS) method for simultaneous determination of artemisinin, arteannuin B, artemisic acid, and scopoletin, and study the pharmacokinetics of the four constituents in mouse serum after oral administration of the four components to mice. The analytical column used was Agilent Zorbax SB-C18 (2.1 mm x 150 mm, 5 mm). The mobile phase was acetonitrile: 0.5% acetic acid (60: 40) and the flow rate was 0.3 mL x min(-1). The temperature of the column was 40.0 degrees C. In this condition, we established an analysis method to simultaneously determine the four components. A sensitive and specific liquid chromatography-mass spectrometric (LC-MS) method was developed and validated for the determination of artemisin in derivatives in mice plasma. The method we established has a linear range of 5-3 000 μg x L(-1) with a good sensitivity and specificity for all of the four components. This method is simple, rapid, accurate and suitable for the determination of the content of the four compounds.
Animals ; Artemisinins ; blood ; pharmacokinetics ; Chromatography, High Pressure Liquid ; instrumentation ; methods ; Dose-Response Relationship, Drug ; Male ; Mice ; Reproducibility of Results ; Scopoletin ; blood ; pharmacokinetics ; Spectrometry, Mass, Electrospray Ionization ; methods

OBJECTIVETo study absorption kinetics of scopoletin in rat stomachs and intestines.

METHODRats was cannulated for in situ recirculation. UV and HPLC methods were used to determine the concentrations of phenolsulfonphthalein and scopoletin, respectively.

RESULTThe absorption rates in rat stomachs at 2 h after administration was 76.31%; The absorption rates at colon, duodenum, ileum and jejunum were 46.25%, 40.54%, 38.21%, 32.77%, respectively. The absorption rate constant (Ka) at concentrations of 10.0144, 20.0288-40.0576 mg x L(-1) in intestine were 0.6434, 0.6137, 0.5970 h(-1), respectively. The Ka of scopoletin at pH of 6.0, 6.8 and 7.4 in intestine were 0.6217, 0.6033, 0.6137 h(-1), respectively.

CONCLUSIONThe concentrations and pH values of scopoletin solution had no distinctive effect on the absorption kinetics. The absorption of scopoletin was a first-order process with passive diffusion mechanism. Scopoletin was well absorbed at stomachs and intestines in rats. Colon was the best absorption site of scopoletin, which suggest that a sustained-release preparation should be suitable for this compound.

Absorption ; Animals ; Chromatography, High Pressure Liquid ; Female ; Hydrogen-Ion Concentration ; Intestinal Absorption ; Intestines ; metabolism ; Male ; Rats ; Rats, Sprague-Dawley ; Scopoletin ; pharmacokinetics ; Spectrophotometry, Ultraviolet ; Stomach ; metabolism