1.Fasciola gigantica Fatty Acid Binding Protein (FABP) as a Prophylactic Agent against Schistosoma mansoni Infection in CD1 Mice.
Ibrahim Rabia ALY ; M DIAB ; A M EL-AMIR ; M HENDAWY ; S KADRY
The Korean Journal of Parasitology 2012;50(1):37-43
Although schistosomicidal drugs and other control measures exist, the advent of an efficacious vaccine remains the most potentially powerful means for controlling this disease. In this study, native fatty acid binding protein (FABP) from Fasciola gigantica was purified from the adult worm's crude extract by saturation with ammonium sulphate followed by separation on DEAE-Sephadex A-50 anion exchange chromatography and gel filtration using Sephacryl HR-100, respectively. CD1 mice were immunized with the purified, native F. gigantica FABP in Freund's adjuvant and challenged subcutaneously with 120 Schistosoma mansoni cercariae. Immunization of CD1 mice with F. gigantica FABP has induced heterologous protection against S. mansoni, evidenced by the significant reduction in mean worm burden (72.3%), liver and intestinal egg counts (81.3% and 80.8%, respectively), and hepatic granuloma counts (42%). Also, it elicited mixed IgG1/IgG2b immune responses with predominant IgG1 isotype, suggesting that native F. gigantica FABP is mediated by a mixed Th1/Th2 response. However, it failed to induce any significant differences in the oogram pattern or in the mean granuloma diameter. This indicated that native F. gigantica FABP could be a promising vaccine candidate against S. mansoni infection.
Animals
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Antibodies, Helminth/immunology
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Fasciola/*chemistry
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Fatty Acid-Binding Proteins/*administration & dosage/immunology/isolation & purification
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Female
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Helminth Proteins/*administration & dosage/immunology/isolation & purification
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Humans
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Immunization
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Mice
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Mice, Inbred Strains
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Schistosoma mansoni/immunology/*physiology
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Schistosomiasis mansoni/immunology/parasitology/*prevention & control
2.Monoclonal Antibody-Based Dipstick Assay: A Reliable Field Applicable Technique for Diagnosis of Schistosoma mansoni Infection Using Human Serum and Urine Samples.
Zeinab DEMERDASH ; Salwa MOHAMED ; Mohamed HENDAWY ; Ibrahim RABIA ; Mohy ATTIA ; Zeinab SHAKER ; Tarek M DIAB
The Korean Journal of Parasitology 2013;51(1):93-98
A field applicable diagnostic technique, the dipstick assay, was evaluated for its sensitivity and specificity in diagnosing human Schistosoma mansoni infection. A monoclonal antibody (mAb) against S. mansoni adult worm tegumental antigen (AWTA) was employed in dipstick and sandwich ELISA for detection of circulating schistosome antigen (CSA) in both serum and urine samples. Based on clinical and parasitological examinations, 60 S. mansoni-infected patients, 30 patients infected with parasites other than schistosomiasis, and 30 uninfected healthy individuals were selected. The sensitivity and specificity of dipstick assay in urine samples were 86.7% and 90.0%, respectively, compared to 90.0% sensitivity and 91.7% specificity of sandwich ELISA. In serum samples, the sensitivity and specificity were 88.3% and 91.7% for dipstick assay vs. 91.7% and 95.0% for sandwich ELISA, respectively. The diagnostic efficacy of dipstick assay in urine and serum samples was 88.3% and 90.0%, while it was 90.8% and 93.3% for sandwich ELISA, respectively. The diagnostic indices of dipstick assay and ELISA either in serum or in urine were statistically comparable (P>0.05). In conclusion, the dipstick assay offers an alternative simple, rapid, non-invasive technique in detecting CSA or complement to stool examinations especially in field studies.
Animals
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Antibodies, Helminth/diagnostic use/isolation & purification
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Antibodies, Monoclonal/diagnostic use/isolation & purification
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Antigens, Helminth/*blood/*urine
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Diagnostic Tests, Routine/*methods
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Humans
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Immunoassay/methods
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Parasitology/*methods
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*Point-of-Care Systems
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Schistosoma mansoni/immunology/*isolation & purification
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Schistosomiasis mansoni/*diagnosis
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Sensitivity and Specificity