1.Guanylyl cyclase C and guanylin reduce fat droplet accumulation in cattle mesenteric adipose tissue.
Masahiro YASUDA ; Jyunya KAWABATA ; Sayaka AKIEDA-ASAI ; Tetsuo NASU ; Yukari DATE
Journal of Veterinary Science 2017;18(3):341-348
Guanylyl cyclase C (GC-C) is a member of a family of enzymes that metabolize GTP to cGMP and was first identified as a receptor for heat-stable enterotoxin. Guanylin (GNY) has since been identified as an endogenous ligand for GC-C in the intestine of several mammalian species. The GNY/GC-C system regulates ion transportation and pH in the mucosa. Recently, it was reported that GC-C and GNY are involved in lipid metabolism in rat mesenteric adipose tissue macrophages. To examine the role of GC-C and GNY in lipid metabolism in cattle, we used a bovine mesenteric adipocyte primary culture system and a coculture system for bovine adipocytes and GNY-/GC-C-expressing macrophages. Fat droplets were observed to accumulate in bovine mesenteric adipocytes cultured alone, whereas few fat droplets accumulated in adipocytes indirectly cocultured with macrophages. We also observed that GC-C was present in bovine mesenteric adipose tissue, and that fat droplet accumulation decreased after in vitro GNY administration. Expressions of mRNAs encoding lipogenic factors decreased significantly in adipocytes after either coculture or GNY administration. These results suggest that the GNY/GC-C system is part of the control system for lipid accumulation in bovine mesenteric adipose tissue.
Adipocytes
;
Adipose Tissue*
;
Animals
;
Cattle*
;
Coculture Techniques
;
Enterotoxins
;
Guanosine Triphosphate
;
Guanylate Cyclase*
;
Humans
;
Hydrogen-Ion Concentration
;
In Vitro Techniques
;
Intestines
;
Ion Transport
;
Lipid Metabolism
;
Macrophages
;
Mucous Membrane
;
Rats
;
RNA, Messenger
2.The Efficacy of Choreitogoshimotsuto for Radiation Hemorrhagic Cystitis
Satoshi TAMADA ; Minoru KATO ; Takeshi YAMASAKI ; Sayaka YASUDA ; Taro IGUCHI
Kampo Medicine 2022;73(2):187-189
We investigated the efficacy of choreitogoshimotsuto for radiation hemorrhagic cystitis. Of the 11 patients who could be followed up, hematuria disappeared in 8 cases. The median time to disappearance of gross hematuria was 74 days. There was no recurrence. Of the 3 ineffective patients, one had urinary diversion, and two had transurethral electrocoagulation and hyperbaric oxygen therapy. There were no adverse events associated with the use of choreitogoshimotsuto. Choreitogoshimotsuto might be effective for radiation hemorrhagic cystitis.