1.Separation of active chemical components against sarcoma 180 from stembark of catalpa ovata.
Han Suk YAUNG ; Min Sun KIM ; Hae Young CHUNG ; Jae Sue CHOI ; Byung Woo KIM
Journal of the Korean Cancer Association 1992;24(6):807-812
No abstract available.
Sarcoma 180*
;
Sarcoma*
2.Antitumor effect of selected medicinal plant compounds to implanted sarcoma 180 in the mouse.
Jae Chung HAH ; Eun Sang CHOE ; Tae Hyong RHEW ; Han Suk YOUNG ; Kun Young PARK
Journal of the Korean Cancer Association 1991;23(2):197-205
No abstract available.
Animals
;
Mice*
;
Plants, Medicinal*
;
Sarcoma 180*
;
Sarcoma*
3.Antitumor effect of linoleic acid against sarcoma 180 detected by the use of protein A-gold complex in mice.
Jae Chung HAH ; Tae Hyung RHEW ; Eun Sang CHOE ; Han Suk YAUNG ; Kun Young PARK
Journal of the Korean Cancer Association 1992;24(6):783-789
No abstract available.
Animals
;
Linoleic Acid*
;
Mice*
;
Sarcoma 180*
;
Sarcoma*
4.In vivo antitumor effects of lactic acid bacteria on sarcoma 180 and mouse lewis lung carcinoma.
Hyung Yong KIM ; Hyeong Suk BAE ; Young Jin BAEK
Journal of the Korean Cancer Association 1991;23(2):188-196
No abstract available.
Animals
;
Bacteria*
;
Carcinoma, Lewis Lung*
;
Lactic Acid*
;
Mice*
;
Sarcoma 180*
;
Sarcoma*
5.Study of 188Re ( V ) -DMSA for Treatment of Cancer - Rediolabeling and Biodistribution.
Chang Soon KOH ; Myung Chul LEE ; June Key CHUNG ; Dong Soo LEE ; Young Ju KIM ; Jae Min JEONG ; Young Soo CHANG
Korean Journal of Nuclear Medicine 1998;32(1):81-88
The purpose of this study was to examine the radiolabeling and biodistribution of 188Re(V)-DMSA as a therapeutic cancer radiopharmaceutical. We made a DMSA kit(NaHC03 1.5 mg, meso-2,3-dimercaptosuccinic acid 1.0 mg L(+)-ascorbic acid 0.7mg, SnCl2 2H2O 0.34 mg, pH 2.9) for labeling with 188Re. In this kit, 188ReO4- 5 mCi/2 ml added and boiled at 100 degree C for 3 hr in water bath. The final pH adjusted to 7.5 with 7% NaHCO3 solution. We checked the labelling efficacy with TLC-SG(n-butanol : acetic acid:H2O = 3 : 2 : 3) and examined the stability both in room temperature and in serum at 37 degree C. Biodistribution(l, 3, 13, 24, 48 hr) of 188Re(V)-DMSA compound was evaluated in Sarcoma 180 tumor-bearing mice. Each labeling efficiency and stability at room temperature for 48 hours was over 98% and 95%, respectely. The stability in serum were 82%(6 hr) and 85%(48 hr). Tumor uptake of 188Re(V)-DMSA in Sarcoma 180- bearing mice were 0.66+/-0.15%(1 hr), 0.51+/-0.10%(3 hr), 0.19+/-0..05%(24 hr) and 0.13+/-0.02%(48 hr). These result are consistent with those of 99mTc(V)-DMSA which were reported previously In conclusion, 188Re(V)-DMSA may be a useful therapeutic radiopharmaceutical for treating some cancers and metastatic bone lesion.
Animals
;
Baths
;
Hydrogen-Ion Concentration
;
Mice
;
Sarcoma
;
Sarcoma 180
;
Succimer
;
Water
6.Observation and research on the electroporation of S-180 cell induced by electric pulse.
Hong LI ; Qin GENG ; Bisong YUE ; Fangdong ZOU ; Baoyi WANG ; Hong ZHANG ; Hao ZENG
Journal of Biomedical Engineering 2005;22(1):47-49
This is our first time report of S-180 cell handled with various voltages, capacity and pulse number. Many pores on the S-180 cell membrane can be observed under scanning electron microscope. By the stains of trypan blue, we have known the influence of electric parameters on the ratio of poration. The results show that the ratio of electroporation has positive correlation with the voltages and the pulse number while negative correlation with the capacity.
Animals
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Cell Membrane Permeability
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Electromagnetic Fields
;
Electroporation
;
methods
;
Mice
;
Sarcoma 180
;
ultrastructure
;
Tumor Cells, Cultured
7.The Optimal Culture Conditions for the Mycelial Growth of Oudemansiella radicata.
Sang Beom KIM ; Seong Hwan KIM ; Kyung Rim LEE ; Jae Ouk SHIM ; Min Woong LEE ; Mi Ja SHIM ; U Youn LEE ; Tae Soo LEE
Mycobiology 2005;33(4):230-234
Oudemansiella radicata, one of edible mushrooms belonging to Tricholomataceae of Basidiomycota, has been known to exhibit outstanding therapeutic effects on the hypertension caused by high blood pressure and inhibitory effects on the sarcoma 180 and Erhrlich carcinoma of mice. As one of preliminary experiments for producing fruiting-body of O. radicata, this study was carried out to obtain the basic information for culture conditions of mycelial growth of the fungus. The optimal temperature and pH for the mycelial growth were 25degrees C and pH 6, respectively. The medium for favorable mycelial growth of O. radicata was shown in the Lilly medium, whereas compact mycelial density was found in Hamada medium. The carbon and nitrogen sources promoting for mycelial growth of O. radicata were xylose and alanine, respectively. The optimum C/N ratio was about 20 : 1 in case that 3% glucose was supplimented to the basal medium as a carbon source.
Agaricales
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Alanine
;
Animals
;
Basidiomycota
;
Carbon
;
Fungi
;
Glucose
;
Hydrogen-Ion Concentration
;
Hypertension
;
Mice
;
Nitrogen
;
Sarcoma 180
;
Xylose
8.Anti-tumor Effects of Exo- and Endo-biopolymers Produced from Submerged Cultures of Three Different Mushrooms.
Yong Tae JEONG ; Byung Keun YANG ; Chun ru LI ; Chi Hyun SONG
Mycobiology 2008;36(2):106-109
The anti-tumor effects of exo- (EX) and endo-biopolymers (EN) produced from submerged mycelial cultures of Ganoderma applanatum (GA), Collybia confluens (CC), and Pleurotus eryngii (PE) were studied using Sarcoma 180 bearing mice. Solid tumor growth was inhibited most effectively when 40 mg/kg body weight (BW) of GA-EX or PE-EN was administered to the intraperitoneal (i.p.) cavity of BALB/c mice. The spleen and liver indexes were increased in mice following i.p. administration of GA-EX and PE-EN fractions. GA-EX and PE-EN reduced the tumor formation by 30.7% and 29.4%, respectively. GA-EX and PE-EN increased the natural killer (NK) cell activity of splenocytes by 41.3% and 28.9%, respectively.
Agaricales
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Animals
;
Biopolymers
;
Body Weight
;
Ganoderma
;
Liver
;
Mice
;
Pleurotus
;
Sarcoma 180
;
Spleen
;
Ursidae
9.Antitumor Effects of Ethanol Extract from Ventilago leiocarpa Benth on Sarcoma 180 Tumor-Bearing Mice and Possible Immune Mechanism.
Dao-Hai CHENG ; Ying LIU ; Li WANG
Chinese journal of integrative medicine 2021;27(12):905-911
OBJECTIVE:
To explore the antitumor effects of ethanol extract from Ventilago leiocarpa Benth (EEVLB) on sarcoma 180 (S180) tumor-bearing mice and the potential mechanism.
METHODS:
Sixty mice were randomly assigned to 6 groups according to a random number table: normal group, model group, 5-fluorouracil (5-FU) group (0.02 g·kg
RESULTS:
EEVLB with different concentrations achieved inhibition of tumor growth in vivo, wherein the high-dose group showed the most significant reduction in tumor weight and increased apoptosis of tumor cells (P<0.05). In addition, both net weight gain and spleen index of mice showed uptrend in EEVLB treatment groups (P<0.05). Besides, serum levels of IL-2 and IL-6, percentages of CD3
CONCLUSIONS
EEVLB exhibits promising antitumor activity in vivo. This effect might be due to activation of apoptotic signaling pathway, increase of cytokine levels and enhancement of immune function in tumor-bearing mice.
Animals
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Cell Line, Tumor
;
Ethanol
;
Mice
;
Plant Extracts/therapeutic use*
;
Rhamnaceae
;
Sarcoma 180/drug therapy*
10.Comparison of therapeutic responses to an anticancer drug in three stocks of ICR mice derived from three different sources.
Ji Eun SUNG ; Ji Eun KIM ; Hyun Ah LEE ; Woo Bin YUN ; Jun Young CHOI ; Mi Rim LEE ; Jin Ju PARK ; Hye Ryeong KIM ; Bo Ram SONG ; Young Suk JUNG ; Kil Soo KIM ; Dae Youn HWANG
Laboratory Animal Research 2017;33(2):187-194
Korl:ICR mice, established by the Korean National Institute of Food and Drug Safety Evaluation (NIFDS), are characterized based on their genetic variation, response to gastric injury, and response to constipation inducers. To compare the inhibitory responses of ICR stocks obtained from three different sources to the anticancer drug cisplatin (Cis), alterations in tumor volume, histopathological structure, and toxicity were examined in Sarcoma 180 tumor-bearing Korl:ICR, A:ICR (USA source), and B:ICR (Japan source) mice treated with low and high concentrations of Cis (L-Cis and H-Cis, respectively). Tumor size and volume were lower in H-Cis-treated mice than in L-Cis-treated mice in all three ICR stocks with no significant differences among stocks. There was a significant enhancement of the necrotizing areas in the histological structures in the L-Cis- and H-Cis-treated groups relative to that in the untreated group. The necrotizing area changes were similar in the Sarcoma 180 tumor-bearing Korl:ICR, A:ICR, and B:ICR mice. However, there were stock-bases differences in the serum biomarkers for liver and kidney toxic effects. In particular, the levels of AST, ALT and BUN increased differently in the three H-Cis-treated ICR stocks, whereas the levels of ALP and CRE were constant. Taken together, the results of the present study indicate that Korl:ICR, A:ICR, and B:ICR mice have similar overall inhibitory responses following Cis treatment of Sarcoma 180-derived solid tumors, although there were some differences in the magnitude of the toxic effects in the three ICR stocks.
Animals
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Biomarkers
;
Cisplatin
;
Constipation
;
Genetic Variation
;
Kidney
;
Liver
;
Mice
;
Mice, Inbred ICR*
;
Sarcoma
;
Sarcoma 180
;
Tumor Burden