BACKGROUNDActivating on mammalian and human body LDR is thought to induce adaptive response, enhance immune function and increase anti-tumor ability. This study was designed to assess the effect of low-dose radiation on tumor growth and on erythrocyte immune function and superoxide dismutase (SOD) activity in tumor-bearing mice.

METHODSMale Kunming mice were subcutaneously implanted with S180 sarcoma cells in the right inguen to create an experimental in situ animal model. Six hours before implantation, the mice were given 75 mGy X-ray radiation, over the body. Tumor size was observed 5 days later while tumor volume was calculated every other day, allowing for the creation of a graph depicting tumor growth. Fifteen days after implantation, the mice were killed to measure tumor weight and observe the necrotic areas and the location of tumor-infiltrating lymphocytes (TILs). Erythrocyte immune function and SOD activity were also determined.

RESULTSMice pre-exposed to low-dose radiation had a lower tumor formation rate than did those receiving no radiation (P < 0.05). Tumor growth was significantly lower in the mice pre-exposed to low-dose radiation; after 15 days, the average tumor weight in the mice pre-exposed to low-dose radiation was also lower (P < 0.05). Areas of tumor necrosis and infiltration of TILs were larger in the low-dose radiation group than in the non-radiation group. Erythrocyte immune function and SOD activity were higher in the low-dose radiation group than in the non-radiation group (P < 0.05).

CONCLUSIONLow-dose radiation can markedly increase the anti-tumor ability of an organism and improve erythrocyte immune function and red blood cell SOD activity as well, suggesting that low-dose radiation might be useful in the clinical treatment of cancer.

Animals ; Biopsy ; Erythrocytes ; enzymology ; immunology ; radiation effects ; Free Radicals ; Male ; Mice ; Sarcoma 180 ; blood ; pathology ; radiotherapy ; Superoxide Dismutase ; blood

This report describes surface properties of several cell membranes tested by lectininduced agglutination reactions which were quantitated using the microquantitative particle counter agglutination assay of Davis et al. (1976). The quantitative assays of concanavaalin A (con A) induced agglutination were performed for rabbit erythrocyte, rat erythrocyte, human erythrocyte, and sarcoma 180 mouse ascites cells. The percent agglutination versus the con A concentration revealed a sigmoid curve in all cases, but the steepness of the sigmoid curve is variable depending on the cell types. It varies even with the same cell but in different species. Optimum cell concentration was (0.92-0.95) x 10(7) cells/ml final concentration in the hanging drop, for rabbit erythrocytes, (0.77-1.64) x 10(7) cells/ml for rat erythrocytes, (1.59-2.7) x 10(7) cells/ml for human erythrocytes and (0.23-0.39) x 10(7) cells/ml for sarcoma 180 mouse ascites cells. When minimal and maximal agglutination percentages were defined as the concentration of con A/ml/1 x 10(6) cells corresponding to 10% and 95% agglutination, minimal and maximal agglutination occured at 0.56 ug, 19.98 ug for human erythrocytes at 0.56 ug, 224 ug for rat erythrocytes at 0.08 ug, 1.43ug for rabbit erythrocytes at 0.12 ug, 14.8 ug for sarcoma 180-mouse ascites cells respectively. The order of inhibitory activity of alpha-methyl-D-mannopyranoside (alphaMM) for each corresponding cells from the highest inhibition was human erythrocytes, rat erythrocytes, sarcoma 180 mouse ascites cells and rabbit erythrocytes. The concentrations of alphaMM required for 50% inhibition per ml of the final concentration in the hanging drop per 1 x 10(7) cells were 0.565 umoles for rabbit erythrocytes, 0.072 umoles for rat erythrocytes, 0.018 umoles for human erythrocytes and 3.677 umoles for sarcoma 180 mouse ascites cells, respectively. From our experimental results we conclude that the cytoagglutination activity was increased with con A, the inhibitory activity with alphaMM in the presence of con A was decreased, however the sarcoma 180 mouse ascites cells revealed a contradictory result, and might be due to the topological distribution of agglutination site changes to a distribution more favorable for agglutination.
Agglutination ; Agglutination Tests ; Animal ; Cell Membrane/immunology* ; Concanavalin A/pharmacology* ; Erythrocytes/immunology ; Human ; Methylmannosides/pharmacology ; Mice ; Rabbits ; Rats ; Sarcoma 180/immunology ; Surface Properties

OBJECTIVETo investigate the therapeutic effect of sequential intratumoral injection of xenogeneic antigens in immunized tumor-bearing mice.

METHODSSequential intratumoral injection of the xenoantigens was performed in immunized mice bearing S180 tumor. The tumor size changes were observed, and the tumor-infiltrating lymphocytes (TIL) including CD3+CD4+T, CD3+CD8+T, and CD3+CD4+CD25+T lymphocytes were counted with flow cytometry. The concentrations of IL-2 and TNF-alpha in the tumor was measured using ELISA.

RESULTSNo significant difference was found in the number of CD3+T lymphocytes in the TILs between different groups. After the immunotherapy, the percentages of CD3+CD4+T, CD3+CD8+T and CD3+CD4+CD25+T lymphocytes were 54%, 22% and 2.91%, respectively, with the CD4+/CD8+ ratio of 2.49, significantly different from that in the control group (P<0.05). The concentrations of IL-2 and TNF-alpha were 100.61 pg/ml and 54.114 pg/ml, respectively, significantly different from those in the control group (P<0.05).

CONCLUSIONSequential intratumoral injection of heteragenetic antigena can significantly increase the amount of effector cells and cytokines in the micro-environment of the tumor, and decrease the expression of T regulatory.

Animals ; Antigens, Heterophile ; immunology ; CD4-CD8 Ratio ; Female ; Immunotherapy ; methods ; Lymphocytes, Tumor-Infiltrating ; cytology ; Male ; Mice ; Random Allocation ; Sarcoma 180 ; immunology ; therapy ; Streptococcus ; immunology

OBJECTIVETo study the erythrocyte immuno-regulatory effect of Patrinia scabra Bunge extracts extracted by macroporous adsorptive resins in tumor bearing mice.

METHODSPatrinia scabra Bunge was extracted by macroporous adsorptive resins, and the amount of polysaccharides and saponins in the extract were determined. Mice bearing S180 tumor were treated with the extract and their survival prolongation rate, erythrocyte rosette formation rates of C3b receptor (ERR-CR), immune complex (ERR-IC) and tumor cell (ERR-TC), as well as the CD35 and CD44s were observed.

RESULTSPolysaccharide content was 21.4%, saponin 41.8% in the extract. As compared with the model group, the survival rate was increased, the erythrocyte immune function was improved (showed increase of ERR-CR and ERR-TC, decrease of ERR-IC), and the amount of CD35 and CD44s in red blood cell membrane increased in mice after being treated with the extract (P < 0.05 or P < 0.01).

CONCLUSIONExtract of Patrinia scabra Bunge extracted by macroporous adsorptive resins can regulate the erythrocyte immune function to a certain extent.

Adsorption ; Animals ; Antineoplastic Agents, Phytogenic ; therapeutic use ; Drugs, Chinese Herbal ; therapeutic use ; Erythrocytes ; cytology ; drug effects ; immunology ; Female ; Male ; Mice ; Patrinia ; chemistry ; Plant Extracts ; chemistry ; therapeutic use ; Receptors, Complement 3b ; immunology ; Resins, Synthetic ; chemistry ; Rosette Formation ; Sarcoma 180 ; drug therapy ; immunology

OBJECTIVETo study the anti-tumor effect and immune function modulation of Xiaoliu decoction (XLD) and to explore the possible mechanism.

METHODSS180 tumor bearing mice were treated with large or small dose of XLD and cyclophosphamide (CTX) to observe the tumor suppressive rate, lymphocyte transformation rate, natural killer cell (NK) activity, tumor necrosis factor alpha (TNFalpha) level in the mice, as well as the tumor cell apoptosis in vitro.

RESULTSThe tumor suppressive rate of XLD on tumor bearing mice was over 30%. XLD showed obvious trend in enhancing the tumor suppressing rate of chemotherapy, could increase the lymphocyte transformation rate, NK activity, and TNFalpha level. It also could accelerate the tumor cell apoptosis in vitro.

CONCLUSIONXLD has significant anti-tumor effect, it could elevate the immune function or organism and alleviate the chemotherapy induced lowering of immune function.

Adjuvants, Immunologic ; pharmacology ; therapeutic use ; Animals ; Antineoplastic Agents, Phytogenic ; pharmacology ; therapeutic use ; Cyclophosphamide ; adverse effects ; therapeutic use ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Female ; Killer Cells, Natural ; immunology ; Lymphocyte Activation ; drug effects ; Male ; Mice ; Phytotherapy ; Sarcoma 180 ; drug therapy ; immunology ; Tumor Necrosis Factor-alpha ; analysis

OBJECTIVETo discuss the effect of the association of Shiquandabutang with Hetaokun on the protection to liver and immunity of tumor mouse S180.

METHODThrough the comparison between tumor transplanted and tumor cultured completely out of cell body, the effect was investigated, with Hetaokun singly used and Shiquandabutang associated with Hetaokun, on the level of SGPT, licking up function of monocyte macrophage, thymus index, spleen index and transform percentage of lymphocyte of the mouse S180. The scanning electron micrographs indicated the protective function of the association of Shiquandabutang with Hetaokun for liver.

RESULT12.5 mg x kg(-1) Hetaokun could obviously restrain the tumor when it was singly used at 73.1%, but it increased SGPT at the same time. The association of Shiquandabutang with Hetaokun could protect liver of the mouse S180. SGPT (P > 0.05) was compared with the common ones and the result showed that the association of shiquandabutang with Hetaokun could make K value increase (P > 0.01). Through the scanning electron micrographs, association of two medicines was found to change liver cells little and expansion of rough Neizhiwang was not found. Compared with the control group, the immunity of the two tumor mouse groups taking the medicine mentioned above was promoted obviously.

CONCLUSIONThe association of Shiquandabutang with Hetaokun can enhance the efficiency and decrease poison of them to body. With the promotion of immunity of the body, the efficiency is enhanced on the whole.

Animals ; Antineoplastic Agents, Phytogenic ; pharmacology ; Benzoquinones ; isolation & purification ; pharmacology ; Drug Combinations ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Fruit ; chemistry ; Juglans ; chemistry ; Liver ; pathology ; Lymphocyte Activation ; drug effects ; Macrophages ; immunology ; Male ; Mice ; Mitochondria, Liver ; drug effects ; Mitochondrial Swelling ; drug effects ; Phagocytosis ; drug effects ; Plants, Medicinal ; chemistry ; Sarcoma 180 ; immunology ; pathology

OBJECTIVETo study the effects of two kinds of cactus polysaccharide on erythrocyte immune function in S180 mice.

METHODClassical pharmaceutical method and test kit.

RESULTThe cactus polysaccharide increased the content of RBC-CaR, RFER, decreased the content of RFIR, raised the content of sialic acid. And the effect of median dose group of medical cactus polysaccharide and high dose group of edible cactus polysaccharide is very remarkable (P < 0.01) compared with model group.

CONCLUSIONThe cactus polysaccharide improved the erythrocyte function of tumor-mice, which may be one of anti-tumor mechanisms.

Animals ; Antineoplastic Agents, Phytogenic ; administration & dosage ; isolation & purification ; pharmacology ; Cactaceae ; chemistry ; Dose-Response Relationship, Drug ; Erythrocytes ; immunology ; metabolism ; Male ; Mice ; N-Acetylneuraminic Acid ; blood ; Neoplasm Transplantation ; Opuntia ; chemistry ; Plants, Edible ; Plants, Medicinal ; chemistry ; Polysaccharides ; administration & dosage ; isolation & purification ; pharmacology ; Random Allocation ; Receptors, Complement 3b ; metabolism ; Rosette Formation ; Sarcoma 180 ; metabolism ; pathology