Mouse hepatoma H22 cells and sarcoma cells (H22 and S180) were infected with EGFP-N1 by electroblot, and the acquired gfp-H22 and gfp-S180 cells expressing strong green fluorescence protein (GFP) fluorescence were supplemented with medium G418 Sigma (800 mg/ml). Meanwhile, the models bearing cancer (gfp H22 and gfp S180) subcutaneously and with abdominal cavity were established. There were no statistically significant differences by comparison on the cell phenotype, ultramicrostructure, growth curve and bearing cancer time between the H22 cells and S180 cells (P>0.05). The GFP fluorescence was detected with whole body GFP imaging system in vivo and with fluorescence microscope. According to the results of in vitro and in vivo assay, it was shown that, by application of fluorescence technology, the GFP-expressing H22 cells and S180 cells could be used in further studies on the tumor biological behavior.
Animals ; Electroporation ; Green Fluorescent Proteins ; genetics ; metabolism ; Liver Neoplasms, Experimental ; genetics ; metabolism ; Mice ; Microscopy, Fluorescence ; Sarcoma 180 ; genetics ; metabolism ; Transfection ; methods ; Tumor Cells, Cultured

OBJECTIVETo observe the pharmacodynamic and side effects of Wulong Kangai, a new drug of Chinese traditional herbal medicine, on 4 strains of mice transplantable tumors.

METHODMice transplantable tumors S180, H22, P388 and Lewis were used in the pharmacodynamic test on the granules of Wulong Kangai. The test on each tumor strain was repeated three times. In each test, 50 mice were used and divided into 5 groups. They were negative control group treated by physiological saline, cyclophosphamide control group and 3 test groups treated respectively with Wulong Kangai at deferent dosages of 10, 25, 40 g x kg(-1) x d(-1) in the treatment of Lewis and P388 and 15, 30, 50 g x kg(-1) x d(-1) in the treatment of S180 and H22.

RESULTThe tumor weight were inhibited at the rates of 90.1%, 30.8%, 49.8% and 52. 3% in the mice with tumors of Lewis, P388, S180, and H22 by high dosage of Wulong Kangai as compared with negative control group. The inhibitory rates in cyclophosphamide groups were 90.6%, 77.2%, 79.6% and 60.3% respectively. The mice body weights grew slower in high dose groups treated by Wulong Kangai granule.

CONCLUSIONWulong Kangai was effective in treating mice transplantable tumors of Lewis, P388, S180 and H22 with a dose-dependent manner. The Lewis was the most sensitive strain to the drug among the 4 kinds of tested tumors. Side effects appeared during 9-11 days of uninterrupted treatment with high dose Wulong Kangai.

Animals ; Antineoplastic Agents ; pharmacology ; toxicity ; Arthropods ; chemistry ; Carcinoma, Lewis Lung ; pathology ; Cell Line, Tumor ; Dose-Response Relationship, Drug ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; toxicity ; Female ; Leukemia P388 ; pathology ; Liver Neoplasms, Experimental ; pathology ; Male ; Materia Medica ; isolation & purification ; pharmacology ; toxicity ; Mice ; Mice, Inbred C57BL ; Mice, Inbred DBA ; Neoplasm Transplantation ; Neoplasms, Experimental ; pathology ; Plants, Medicinal ; chemistry ; Sarcoma 180 ; pathology

OBJECTIVETo study the anti-tumor angiogenesis effect of soluble VEGF receptor fragment by blocking the combination of VEGF and its receptor in vivo and in vitro.

METHODSRT-PCR technique was used to amplify Flk-1/KDR fragment from embryo mouse liver, which was recombinated to expression vector pET-28b(+) and retrovirus vector PLXSN, which was induced to be expressed, purified and identified with EcoR I and Hind III. Mouse endothelial cells were separated, cultured and identified by immunocytochemistrical staining using VIII factor-related antigen antibody. The expressed product was analyzed about its effect on endothelial cell's growth in vitro with MTT method. The retrovirus vector was transfected to tumor cell lines S180 and B16 by liposome method to observe the biological specificity in vitro after gene transfection.

RESULTS1000 bp size sVEGFR fragment was amplify from E9, E11 embryo mouse liver tissues, which was recombinated to TA clone vector and identified by sequence analysis. This fragment was cloned to expression vector pET-28b(+), the expressed product was purified and identified correctly. The in vitro study showed this expressed product can effectively inhibit endothelial cell(s), growth and proliferation. The fragment was then cloned to retrovirus vector PLXSN and transfected to tumor cell lines S180 and B16 successfully with RT-PCR and SDS-PAGE. The experiments in vivo showed that the weight of tumor smaller, the size decreased significantly, the microvessel density was fewer and Flk1 protein expression were higher in the group of gene transfection than that of control.

CONCLUSIONSoluble VEGFR fragment is a kind of effective gene engineer product for anti-tumor angiogenesis gene therapy and the development of anti-tumor drug.

Animals ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Cloning, Molecular ; Endothelial Cells ; cytology ; Genetic Vectors ; Melanoma, Experimental ; metabolism ; pathology ; Mice ; Mice, Inbred BALB C ; Neoplasm Transplantation ; Neovascularization, Pathologic ; pathology ; RNA, Messenger ; biosynthesis ; genetics ; Retroviridae ; genetics ; Sarcoma 180 ; metabolism ; pathology ; Transfection ; Vascular Endothelial Growth Factor Receptor-2 ; biosynthesis ; genetics ; physiology

OBJECTIVETo observe the anti-tumor activity of the ethanol extracts of Solanun lyratum in vitro and in vivo.

METHODIn vitro, the inhibitory effects of ethanol extracts of S. lyratum on proliferation of human hepatoma BEL-7402 cell and gastric carcinoma SGC-7901 cell were measured by MTT colorimetric assay. The mouse tumor model was used to investigate the effects of ethanol extracts on tumor growth.

RESULTThe studies demonstrated that ethanol extracts of S. lyratum inhibited proliferation of BEL-7402 cells and SGC-7901 cells, and the IC50 values on them were (287.40 +/- 5.84) micron x mL(-1) and (176.14 +/- 5.18) microg x mL(-1), respectively. The tumor inhibitory rate of high doses of ethanol extracts on S180 sarcoma-transplanted mice and H22 hepatic cancer were (41.15 +/- 4.54) % and (45.00 +/- 7.37) %, respectively. When the dose of ethanol extracts varied from low to high, it was able to inhibit the growth of S180 sarcoma-transplanted mice and H22 hepatic cancer in a dose-dependent manner.

CONCLUSIONIn tumor inhibitory test, it was shown that the ethanol extracts of S. lyratum may possess significantly inhibitory effect in vitro and in vivo. No acute toxic effect was found in our experiment.

Adenocarcinoma ; pathology ; Animals ; Antineoplastic Agents, Phytogenic ; isolation & purification ; pharmacology ; Carcinoma, Hepatocellular ; pathology ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Dose-Response Relationship, Drug ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Ethanol ; Female ; Humans ; Liver Neoplasms ; pathology ; Liver Neoplasms, Experimental ; pathology ; Male ; Mice ; Neoplasm Transplantation ; Plants, Medicinal ; chemistry ; Sarcoma 180 ; pathology ; Solanum ; chemistry ; Stomach Neoplasms ; pathology

OBJECTIVETo study the anti-tumor activity of extractive from Celastrus orbiculatus in vivo.

METHODMice bearing transplanted tumor S180 and Heps were used to study the effects of acetoacetate and n-butanol extractive from C. orbiculatus. The changes in serum contents of SOD and malondialdehyde (MDA) content were assayed.

RESULTAcetoacetate and n-butanol extractive from C. orbiculatus significantly inhibited the growth of S180 and Heps tumor in mice. SOD content was obviously increased, MDA content obviously decreased in the serum after extractive treatment.

CONCLUSIONAcetoacetate and n-butanol extractive from C. orbiculatus have anti-tumor effects and anti-oxidative capacity.

Animals ; Antineoplastic Agents, Phytogenic ; isolation & purification ; pharmacology ; Antioxidants ; isolation & purification ; pharmacology ; Celastrus ; chemistry ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Female ; Liver Neoplasms, Experimental ; blood ; pathology ; Male ; Malondialdehyde ; blood ; Mice ; Mice, Inbred ICR ; Neoplasm Transplantation ; Plant Stems ; chemistry ; Plants, Medicinal ; chemistry ; Sarcoma 180 ; blood ; pathology ; Superoxide Dismutase ; blood

OBJECTIVETo study the antitumor effects of total-flavonoid from S. chamaejasmel.

METHODThe in vitro antitumor activity against human cancer cell lines, such as stomach cancer SGC-7901, hepatocarcinoma BEL-7402 and leukemia HL-60, were determined by a MTT and clone formation assay. The in vivo antitumor activity was evaluated by the antitumor bioassay against transplanted mouse solid tumor S180 and H22.

RESULTThe total-flavonoid inhibited cell proliferation of human tumor cell lines, and its activities are higher than that of vincristine. The total-flavonoid also showed a lower acute toxicity and the strong antitumor activity against transplanted mouse solid tumor S180 and H22 in vivo showing a positive correlation with the concentration. The inhibitory rates at the dose of 0.10 g x kg(-1) ip against S180 and H22 are 45.64% and 47.59%, respectively.

CONCLUSIONThe total-flavonoid from S. chamaejasme has antitumor activities in vivo and in vitro.

Animals ; Antineoplastic Agents, Phytogenic ; administration & dosage ; isolation & purification ; pharmacology ; Carcinoma, Hepatocellular ; pathology ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Dose-Response Relationship, Drug ; Female ; Flavonoids ; administration & dosage ; isolation & purification ; pharmacology ; HL-60 Cells ; Humans ; Liver Neoplasms ; pathology ; Liver Neoplasms, Experimental ; pathology ; Male ; Mice ; Mice, Inbred BALB C ; Neoplasm Transplantation ; Plants, Medicinal ; chemistry ; Sarcoma 180 ; pathology ; Stomach Neoplasms ; pathology ; Thymelaeaceae ; chemistry

OBJECTIVETo study the anticancer activity of the extract from Citrus reticulata in vivo.

METHODAnticancer activities were tested with tumor model in vivo (Sarcoma-180 cells, Heps cells, EAC cells implanted in mice).

RESULTThe extract from Citrus reticulata showed marked anticancer activities on Sarcoma-180 cells and Heps cells implanted in mice, had no marked anticancer activities on EAC cells implanted in mice and induced apoptosis of Sarcoma-180 cell.

CONCLUSIONThe extract from Citrus reticulata will have promising prospects as an anticancer Chinese medicine, but further studies will be needed.

Animals ; Antineoplastic Agents, Phytogenic ; isolation & purification ; pharmacology ; Apoptosis ; drug effects ; Carcinoma, Ehrlich Tumor ; drug therapy ; pathology ; Cell Cycle ; drug effects ; Cell Line, Tumor ; Citrus ; chemistry ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Female ; Fruit ; chemistry ; Liver Neoplasms, Experimental ; drug therapy ; pathology ; Male ; Mice ; Neoplasm Transplantation ; Plants, Medicinal ; chemistry ; Sarcoma 180 ; drug therapy ; pathology

Camptothecin/*pharmacology ; Liver Neoplasms, Experimental/*immunology ; Neoplasm Transplantation ; Sarcoma, Experimental/*immunology ; Transplantation, Isogeneic

Animals ; Camptothecin ; pharmacology ; Liver Neoplasms, Experimental ; immunology ; Mice ; Neoplasm Transplantation ; Sarcoma, Experimental ; immunology ; Transplantation, Isogeneic

No abstract available.
Sarcoma 180* ; Sarcoma*