The cDNA of endo-1,4-beta-xylanaseA, isolated from Phaenerocheate chrysosporium was expressed in Pichia pastoris. Using either the intrinsic leader peptide of XynA or the alpha-factor signal peptide of Saccharomyces cerevisiae, xylanaseA is efficiently secreted into the medium at maximum concentrations of 1,946 U/L and 2,496 U/L, respectively.
Chrysosporium ; DNA, Complementary ; Phanerochaete ; Pichia ; Polysaccharides ; Protein Sorting Signals ; Saccharomyces cerevisiae

cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces H2O2 over 7.0 nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of H2O2 improved the coupled reaction with recombinant manganese peroxidase resulting in decolorization of malachite green up to 150 microM within 90 min.
Alcohol Oxidoreductases ; DNA, Complementary ; Glyoxal ; Manganese ; Organometallic Compounds ; Oxidoreductases ; Peroxidase ; Peroxidases ; Phanerochaete ; Pichia ; Rosaniline Dyes