Study on 335 cases underwent endoscopic biopsies. The results showed that: The rate of Helicobacter pylori (HP) infection in gastric ulcer patients that had clinical symptoms were 63%, highest in group of 31 to 50 years old. Inflammatory and ulcer lesions were in antropyloric area, and the rate of HP (+) was highest in this area. In comparison with endoscopic diagnosis, the highest ratio of HP was in gastric ulcer accompany with duodenal bulb ulcer, and lowest rate was in acute inflammation. There was no significant difference in infected frequency between male and female. However, moderate and severe infections (Hp2+, Hp3+) in men were more common than those in women
Helicobacter pylori ; Stomach Ulcer

The study determined the expression of the biological marker PCNA in invasive breast carcinoma patients treated at Oncology Hospital and HCMC Medical and Pharmaceutical University Hospital from January 2003 to January 2004. The results found out the correlation of the marker with the basic prognostic factors such as: age of patients, histopathological features, histological grade, and lymph node metastases. Among 371 specimens of invasive breast carcinoma which were stained with immunohistochemical technique, there were 96.2% specimens have had the overexpression of PCNA in the cancer cells. However, there was not relation between the overexpression of PCNA and the basic prognostic factors, therefore, it is not necessary to find out the expression of PCNA in all cases of invasive breast carcinoma
Breast Neoplasms ; Proliferating Cell Nuclear Antigen ; Biological Markers

Tumors of central nervous system are common, especially in children. The most common primary tumor is astrocytoma. Study of microscopic features of astrocytoma could help to diagnose and assess histologically this type of tumor more precisely. Subjects include 122 patients with astrocytoma diagnosed at Department of Pathology, Cho Ray Hospital from June 2003 to June 2004. Histological criteria of these 122 cases were atypical nuclear: 122 cases (100%), endothelial proliferation: 69 (56.5%), necrosis: 36 (29.5%), mitoses: 17 (13.9%), micro cystic: 17 (13.9%), calcification: 6 (4.9%), pseudorosettes: 8 (6.5%). The criteria such as atypical nuclear, endothelial proliferation, necrosis, mitoses, and cellularity are important factors in the diagnosis and histological grading of astrocytoma
Astrocytoma ; Central Nervous System Neoplasms ; Diagnosis ; Child

Study 480 cases of rectocolon endoscopic biopsies, to compare the clinical features with the features and microscopic diagnosis. Results: the patients between 50 and 70 years old had the rate of highest catch a diseases and malignant lesions. There was no difference in the cancer ratio between male and female. The percentages of 4 groups included: malignant tumors, inflammatory lesions, polyps and benign tumors, were about 48%, 30%, 13.75% and 6.87%. Malignant tumors occur mainly in the rectum (65.36%) and in the sigmoid colon (9.95%). Most of malignant tumors in the colon and rectum (96%) were adenocarcinoma. The true-positive percentage of endoscopic diagnosis (confronted with microscopic finding) in the cancer group was 70.56%
diagnosis ; endoscopy ; Biopsy ;

BACKGROUND: Gastrointestinal stromal tumors (GISTs) are the most frequent mesenchymal neoplasms of the gastrointestinal tract. Management of GIST patients is currently based on clinicopathological features and associated genetic changes. However, the detailed characteristics and molecular genetic features of GISTs have not yet been described in the Vietnamese population.METHODS: We first identified 155 patients with primary GIST who underwent surgery with primary curative intent between 2011 and 2014 at University Medical Center at Ho Chi Minh City, Vietnam. We evaluated the clinicopathological features and immunohistochemical reactivity to p53 and Ki-67 in these patients. Additionally, KIT genotyping was performed in 100 cases.RESULTS: The largest proportion of GISTs was classified as high-risk (43.2%). Of the 155 GISTs, 52 (33.5%) were positive for Ki-67, and 58 (37.4%) were positive for p53. The expression of Ki-67 and p53 were correlated with mitotic rate, tumor size, risk assessment, and tumor stage. Out of 100 GIST cases, KIT mutation was found in 68%, of which 62 (91.2%) were found in exon 11, two (2.9%) in exon 9, and four (5.8%) in exon 17. No mutation in exon 13 was identified. Additionally, KIT mutations did not correlate with any clinicopathological features.CONCLUSIONS: The expression of Ki-67 and p53 were associated with high-risk tumors. Mutations in exon 11 were the most commonly found, followed by exon 17 and exon 9. Additionally, KIT mutation status was not correlated with any recognized clinicopathological features.
Academic Medical Centers ; Asian Continental Ancestry Group ; Exons ; Gastrointestinal Stromal Tumors ; Gastrointestinal Tract ; Humans ; Molecular Biology ; Risk Assessment ; Vietnam

BACKGROUND: Human epidermal growth factor receptor 2 (HER2) is related to the pathogenesis and poor outcome of numerous types of carcinomas, including gastric carcinoma. Gastric cancer patients with HER2 positivity have become potential candidates for targeted therapy with trastuzumab. METHODS: We investigated 208 gastric cancer specimens using immunohistochemistry (IHC), fluorescence in situ hybridization and dual in situ hybridization (ISH). We also investigated the concordance between IHC and ISH. The correlation between HER2 status and various clinicopathological findings was also investigated. RESULTS: In total, 15.9% (33/208) and 24.5% (51/208) of gastric cancers showed HER2 gene amplification and protein overexpression, respectively. A high level of concordance between ISH and IHC analyses (91.3%, κ = 0.76) was found. A significant correlation between HER2 status and intestinal-type (p < .05) and differentiated carcinomas (p < .05) was also noted. The HER2 heterogeneity was high in gastric cancers; we found 68.8% phenotypic heterogeneity and 57.6% genotypic heterogeneity. Heterogeneity in HER2 protein expression and gene amplification showed a close association with diffuse histologic type and IHC 2+. CONCLUSIONS: HER2 protein overexpression and gene amplification were detected in 24.5% and 15.9% of gastric cancer specimens, respectively. Intestinal-type showed a higher level of HER2 protein overexpression and gene amplification than diffuse type. HER2 status also showed a significant relationship with well- and moderately-differentiated carcinomas. The ratio of phenotypic and genotypic heterogeneity of HER2 was high in gastric carcinomas and was associated with HER2 IHC 2+ and diffuse histologic type.
Asian Continental Ancestry Group* ; Fluorescence ; Gene Amplification ; Genes, erbB-2 ; Humans ; Immunohistochemistry ; In Situ Hybridization ; Population Characteristics* ; Receptor, Epidermal Growth Factor ; Stomach Neoplasms ; Trastuzumab