Objective To explore the efficacy of precision intracoronary thrombolysis via micro-catheter in the treatment of acute myocardial infarction(AMI)in the elderly based on inhibiting ventricular remodeling and myocardial protection.Methods A total of 106 elderly AMI patients admitted to our hospital from January 2022 to January 2023 were enrolled,and randomly divided into thrombolysis group(50 patients)and primary PCI group(56 patients).Echocardiography was used to measure the left ventricular end-diastolic diameter(LVEDD),left ventricular end-systolic diameter(LVESD),left atrial diameter(LAD),and left ventricular ejection fraction(LVEF)be-fore and 1 month after operation.Levels of creatine kinase isoenzymes-MB(CK-MB),high-sensitivity cardiac troponin T(hs-cTnT),and high sensitivity C-reactive protein(hs-CRP)were assessed.Major adverse cardiac events(MACE)within 30 d after operation were observed,and ad-verse drug reactions were observed.Results The primary PCI group had significantly lower LVEDD,LVESD and LAD values(40.08±5.18 mm vs 47.22±5.08 mm,29.12±5.07 mm vs 35.33±5.13 mm,33.07±5.07 mm vs 40.08±5.18 mm,P＜0.01),while obviously higher LVEF value[(55.08±5.17)％vs(49.07±5.19)％,P＜0.01]than the thrombolysis group.LVEDD,LVESD and LAD values were notably decreased and that of LVEF was remarkably increased in both groups after operation(P＜0.05).Statistical differences were observed in CK-MB,hs-cTnT and hs-CRP levels between the two groups before and 8 and 24 h after procedure(P＜0.01),with the levels at 8 and 24 h postoperatively significantly higher than those before operation,and the levels at 24 h obviously higher than those at 8 h after operation(P＜0.05).Additionally,the inci-dence of MACE was significantly higher in the primary PCI group than the thrombolysis group(16.1％vs 4.0％,P＜0.05).Conclusion Microcatheter intracoronary thrombolysis can signifi-cantly improve ventricular remodeling in elderly AMI patients and exert a myocardial protective role.

OBJECTIVE：To investigate the effects of dihydroartemisinin （DHA）on the metabolism of amino acid metabolites in human hepatocellular carcinoma cells Huh 7 and BEL- 7402，and to provide theoretic basis for clarifying the mechanism of DHA regulating the metabolism of hepatocellular carcinoma cells. METHODS ：CCK-8 method was taken to detect the effect of different concentrations of DHA （12.5，25，50，100 µmol/L）treating for 24，48，72 h on the two kinds of cells. Two kinds of cells were divided into control group and administration group （DHA，25 µmol/L），and then cultured with drug-free or drug-containing medium for 24 h，operated in parallel for three times. After derivatization of cell samples in each group ，GC-MS method was used to detect the content of amino acid metabolites ，combined with SIMCA-P software analysis and compound library comparison ，the differential metabolites in two kinds of cells were screened out. The pathway enrichment analysis of differential metabolism was conducted with Metaboanalyst 4.0 software. RESULTS ：Compared with control group ，the contents of glutamine ，glutathione， phenylalanine，fumaric acid and taurine were trending down in Huh 7 or BEL- 7402 cells. There were 28 and 29 differential metabolites obtained from the above two kinds of cells ，and 10 of them were common differential metabolites ，including glutamine，glutathione，taurine，fumaric acid ，phenylalanine，etc. The differential metabolites were enriched in 8 and 6 pathways respectively. The common enrichment pathways were amino acid-tRNA biosynthesis ，aspartate-alanine-glutamate metabolism ， nitrogen metabolism ，phenylalanine metabolism and pentose phosphate pathway ，etc. CONCLUSIONS ：DHA can significantly reduce the activities of Huh 7 cells and BEL- 7402 cells，and the contents of glutamine ，glutamic acid ，glutathione and phenylalanine，etc. It may regulate the growth of the two kinds of cells by influencing the mechanism of aspartic acid- alanine-glutamate metabolic pathway ，etc.