A foodborne poisoning incident occurred in a street， Ouhai District， Wenzhou City， Zhejiang Province on May 9， 2023， which was caused by the accidental consumption of medicinal liquor containing aconitum alkaloids. Three individuals drank homemade aconite-containing medicinal liquor and were subsequently poisoned， of which two cases were severe， and one case was mild. All three patients exhibited symptoms such as numbness in the mouth， tongue and limbs， dizziness， fatigue， nausea， vomiting， and blurred vision. Two of them successively entered a state of shock and coma， with an incubation period of approximately 30 minutes. Among seven samples of suspected homemade medicinal liquor， one sample tested positive simultaneously for aconitine， neaconitine， hypaconitine， and benzoylaconitine， with concentrations of 62.3 mg·L^-1， 2.6 mg·L^-1， 17.7 mg·L^-1， and 31.6 mg·L^-1， respectively. Four kinds of aconite alkaloids were detected in all the urine， blood， and vomit samples from the patients， and the most toxic aconitine was detected at levels of 1.5 μg·L^-1， 5.9 μg·L^-1， and 26.2 μg·L^-1， respectively. Based on the field epidemiological investigation， combined with clinical manifestations and laboratory test results， it was determined that this incident was a case of food poisoning caused by the accidental consumption of medicinal liquor containing aconite. Due to timely rescue and proper treatment measures， all poisoned patients have recovered， with no fatalities. It is suggested that relevant authorities should strengthen the regulation of the processing and sale of aconite herbs and promote the awareness of food safety.

ObjectiveTo establish an ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)-internal standard method for the simultaneous determination of 59 antibiotics, including quinolones, sulfonamides, tetracyclines, macrolides and nitroimidazole in bean sprouts. MethodsThe internal standard compounds and 5.0 mL of acetonitrile containing 0.1% formic acid were added into 5.0 g of the sample, and then extracted by ultrasonication. The extracted supernatant was filtered by 0.22 µm membrane and then injected into 1.0 µL of the sample directly. Methanol containing 0.1% formic acid and 2.0 mmol·L^-1 ammonium formate containing 0.1% formic acid were taken as the mobile phases to conduct gradient elution. The separation was achieved on an ACQUITY PREMIER BEH C₁₈ column. The separated analytes were detected by the electrospray ionization (ESI) in multiple reaction monitoring (MRM) mode, and quantified by internal standard method. ResultsThe 59 kinds of antibiotics in bean sprouts had a good linear relationship in the range of 10 μg·kg^-1 to 1 000 μg·kg^-1. The limits of detection (LODs) and the limits of quantification (LOQs) of the method for soybean sprouts ranged from 0.1 μg·kg^-1 to 3.0 μg·kg^-1 and 0.4 μg·kg^-1 to 10.0 μg·kg^-1, respectively. While, the LODs and LOQs of the method for mung bean sprouts ranged from 0.1 μg·kg^-1 to 2.0 μg·kg^-1 and 0.4 μg·kg^-1 to 7.0 μg·kg^-1, respectively. The average recoveries of the 59 kinds of antibiotics at four levels (10、50、200、800 μg·kg^-1) in soybean sprouts were 82.1%‒122.3%, with the relative standard deviations (RSDs) of 1.2%‒15.4% (n=6). However, the average recoveries of the 59 kinds of antibiotics in mung bean sprouts were 87.3%‒119.1%, with the RSDs of 0.9%‒15.0% (n=6). ConclusionThe method is rapid, highly sensitive and accurate, which can be used for rapid screening of quinolones, sulfonamides, tetracyclines, macrolides, and nitroimidazole antibiotics in large quantities of bean sprouts.