Objective To analyze the expression characteristics of SRPK2 ( serine /arginine-rich protein specific kinase 2 mRNA and its encoded protein products in mouse testis, and to reveal its molecular role during spermatogenesis. Methods Using semi-quantitative reverse transcription polymerase chain reaction ( RT-PCR) and Western blotting to an-alyze the expression of SRPK2 mRNA and its protein products in several mouse tissues.The stage-specific expression pat-tern of SRPK2 mRNA in mouse testis was examined by real-time quantitative PCR.Immunohistochemical staining was ap-plied to identify the SRPK2 protein distribution in mouse testicular seminiferous tubules and indirect immunofluorescence staining was used to detect the subcellular localization of SRPK2 protein in spermatic cells.Results Semi-quantitative RT-PCR and Western blotting analysis revealed that SRPK2 was highly expressed in mice testis.Real-time quantitative PCR analysis showed that SRPK2 mRNA was expressed abundantly at the stage of 5-and 8-week old mouse testes, suggesting that SRPK2 gene has stage-specific expression patterns during mouse spermatogenesis.Immunohistochemical and indirect immu-nofluorescence staining demonstrated that SRPK2 protein was located around the surface of elongated sperm nucleus. Conclusions SRPK2 is highly expressed in mouse testis and has significant stage-specific expression characteristics with distinct nuclear localization.These results indicate that SRPK2 may participate in precursor mRNA splicing during mouse spermiogenesis.The molecular mechanism of SRPK2 is yet to be further studied.

Objective:To investigate the prevalence of Work-related musculoskeletal disorders(WMSDs) and identify associated risk factors and provide evidence of taking measures for prevention.Methods:By Cluster sampling, 1 920 employees of 35 manufacturing enterprises were selected as study subjects from May to December 2017. The questionnnaire were including population characteristics、related factors of ergonomics、job burnout、insomnia and WMSDs.Results:The prevalence of waist was the highest among 9 body parts, with 52.0%, followed by the prevalence of neck 50.6%, more than 1 muscle diseases were by the prevalence of 74.7%. Single factor analysis showed that different levels of labor intensity, daily standing time (h) , daily sitting time(h) , daily bending time (h) , daily lifting of 5kg object time (h) group waist disease and prevalence of more than one musculoskeletal disease were different, The difference was statistically sig- nificant ( P<0.05). More than 5 years of working year, labor intensity of Class III, daily bending time ≥2 h, depression, disorders ( P<0.05). life satisfaction, job burnout, and insomnia are all risk factors for waist and musculoskeletal. The daily sitting time ≥2 h and the high life satisfaction were protective factors for waist and WMSDs ( P<0.05). Conclusion:The prevalence rate of WMSDs among manufacturing workers is strong, and Manager should take the aspects of ergonomics and psychological factors meassure to reduce WMSDs, These meassure were including increasing sitting time, reducing bending time.

Objective:To investigate the prevalence of Work-related musculoskeletal disorders(WMSDs) and identify associated risk factors and provide evidence of taking measures for prevention.Methods:By Cluster sampling, 1 920 employees of 35 manufacturing enterprises were selected as study subjects from May to December 2017. The questionnnaire were including population characteristics、related factors of ergonomics、job burnout、insomnia and WMSDs.Results:The prevalence of waist was the highest among 9 body parts, with 52.0%, followed by the prevalence of neck 50.6%, more than 1 muscle diseases were by the prevalence of 74.7%. Single factor analysis showed that different levels of labor intensity, daily standing time (h) , daily sitting time(h) , daily bending time (h) , daily lifting of 5kg object time (h) group waist disease and prevalence of more than one musculoskeletal disease were different, The difference was statistically sig- nificant ( P<0.05). More than 5 years of working year, labor intensity of Class III, daily bending time ≥2 h, depression, disorders ( P<0.05). life satisfaction, job burnout, and insomnia are all risk factors for waist and musculoskeletal. The daily sitting time ≥2 h and the high life satisfaction were protective factors for waist and WMSDs ( P<0.05). Conclusion:The prevalence rate of WMSDs among manufacturing workers is strong, and Manager should take the aspects of ergonomics and psychological factors meassure to reduce WMSDs, These meassure were including increasing sitting time, reducing bending time.