Objective To explore the security of propofol used in internal carotid artery anesthesia by observing the effect of internal carotid artery infusion of propofol on neurovascular tissue of cerebral infusion areas in rats.Methods A total of 40 SD rats were randomly divided into a V group and an A group (n=20); rats in the V group were given treatment of right femoral vein infusion of 30 mg/ (kg· h) propofol,and those in the A group were given treatment of right internal carotid artery infusion of 30 mg/(kg· h) propofol for 3 h.Rats were sacrificed 4 h after propofol withdrawal.HE staining and transmission electron microscopy were employed to detect the morphological and ultrastructural changes in the right cerebral cortex tissues; and semi-quantitative real time PCR was used to observe the mRNA expressions of bcl-2 and Bax.Results No obvious acute injury was noted in both groups,and the morphology of cortical neurons,neuralgia cells and vascular structures was normal in both groups.And the neuronal nuclei,organelles and capillary endomembrane of both groups were normal,without obvious changes.No significant difference in mRNA expressions ofbcl-2 and Bax was noted between the two groups (P＞0.05).Conclusion It is insignificant in the effects of internal carotid artery infusion of propofol on neurovascular tissues of cerebral infusion areas in rats.

Objective To utilize the magnetic resonance neurography (MRN) as a feasible tool for measuring the anatomical parameters of lumbar spinal nerves, and further to evaluate the neuro-safety of interlaminar percutaneous endoscopic lumbar discectomy.Methods Thirty healthy adult volunteers without significant history of low back pain or lumbar deformity were selected in our hospital from September 2016 to December 2016. All subjects accepted MRN. The nerve roots of L2-S1 were measured at the starting point of dural sac, and the angles between nerve roots and dural sac were measured. The distances between L2-L5 nerve roots and the edge of ipsilateral dural sac were measured and analyzed statistically.Results All MRN showed a gradual increase in the origin of the nerve roots from L2 to S1. The origin of the root was found to be below the corresponding disc for the L2 to L4 roots. There were 70％ of the L5 roots originated below the L4/5 disc, 26.7％ at the L4/5 disc, and 3.3％ above the L4/5 disc; about 70％ of the S1 roots originated above the L5/S1 disc. There were no statistically significant differences in the angles between dural sac and both left and right nerve roots (P>0.05). The angels between the nerve root and the dural sac from L5 and S1 was smaller than those from L2, L3, and L4 (P<0.05); that from S1 was significantly smaller than that from L5 (P<0.05). The distance of the nerve root and the ipsilateral dural sac was significantly increased in each side from L2 to L5 (P<0.05). There was no statistically significant difference in the distances between the left and right nerve roots and the edge of the ipsilateral dural sac in the same segment (P>0.05).Conclusion MRN is a feasible tool to measure the anatomical parameters of the lumbar spinal nerve, and there is a safe neurological area of the percutaneous endoscopic lumbar discectomy through the interlaminar approach.