1.Phytochemical investigation and in vitro antioxidant activity of an indigenous medicinal plant Alpinia nigra B.L. Burtt
Suprava SAHOO ; Goutam GHOSH ; Debajyoti DAS ; Sanghamitra NAYAK
Asian Pacific Journal of Tropical Biomedicine 2013;(11):871-876
Objective: To investigate antioxidant potential of methanol extract of Alpinia nigra leaves.Methods:picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), nitric oxide and hydrogen peroxide radical scavenging assays. Phytochemical constituents, total phenolic content and total flavonoid content of the extract at different concentrations (10-500 μg/mL) were determined.Results: Alpinia nigra leaves showed high free radical scavenging activity as evidenced by the low IC50 values in DPPH (64.51 μg/mL), in ABTS (28.32 μg/mL), in nitric oxide (80.02 μg/mL) and in H2O2 (77.45 μg/mL) scavenging assays. Furthermore the TPC and TFC of the extract were found to be 69.25 mg gallic acid equivalent per gram of extract and 78.84 mg quercetin equivalent per gram of extract respectively.Conclusions:The results of present comprehensive analysis demonstrated that Alpinia nigra The study was done by using various in vitro methods such as 1,1-diphenyl-2-leaves possess high phenolic, flavonoid contents and potential antioxidant activity, and could be used as a viable source of natural antioxidants and might be exploited for functional foods and neutraceutical applications.
2. Biotechnological intervention in betelvine (Piper betle L.): A review on recent advances and future prospects
Suryasnata DAS ; Reena PARIDA ; Reena SRIRAM SANDEEP ; Sanghamitra NAYAK ; Sujata MOHANTY
Asian Pacific Journal of Tropical Medicine 2016;9(10):938-946
Betelvine (Piper betle L.) is cultivated for its deep green heart shaped leaf for (15–20) million Indian and 2 billion foreign consumers annually. The crop provides Rs (6 000–7 000) million of national income per year and at the same time leaves worth Rs (30–40) million is exported to other countries. The leaves are not only used directly for chewing purposes but also possesses antioxidant, anti-inflammatory, anti-apoptotic, anti-cancer and anti-microbial properties. Besides, the leaves also contain eugenol rich essential oil (1%–3%) which is the source for medicine, stimulant, antiseptic, tonic and other ayurvedic formulations. The essential oil also contains chavibetol, caryophyllene and methyl eugenol which are the potent source for preparation in ayurvedic medicine and herbal products. Cost of betelvine essential oil is 10$ per 5 mL. In spite of its great economical and medicinal importance betelvine is still neglected by the researchers for proper characterization and authentication for selection of elite landraces. Lack of awareness among people, use of same planting material for many generations, existing of many synonyms for a single landraces, no proper characterization of available landraces are some of the significant constraints for its commercialization. Our review endeavours a complete advance in the research on betelvine, existing lacunae for its proper characterization and commercial cultivation. It also attempts to provide a comprehensive account on biotechnological interventions made in betelvine aimed at complementing conventional programmes for improvement of this nutraceutically important cash crop.
3. Curcuma angustifolia ameliorates carbon tetrachloride-induced hepatotoxicity in HepG2 cells and Swiss albino rats
Sudipta JENA ; Asit RAY ; Ambika SAHOO ; Subhashree SINGH ; Noohi NASIM ; Sanghamitra NAYAK ; Diptirani RATH ; Durga Madhab KAR
Asian Pacific Journal of Tropical Medicine 2019;12(9):416-424
Objective: To determine the antioxidant and hepatoprotective potential of methanol extract of rhizome of Curcuma angustifolia (MECA) against carbon tetrachloride (CCl