1.Laboratory diagnosis of tuberculosis using non-immunological tools.
Tuberculosis and Respiratory Diseases 1991;38(3):217-221
No abstract available.
Clinical Laboratory Techniques*
;
Tuberculosis*
2.Seroactivities to Phenolic Glycolipid I of Mycobacterium leprae Among Leprosy Patients in National sorokdo Hospital and Their Children.
Kyu Kwang HWANG ; Sang Nae CHO
Korean Journal of Dermatology 1988;26(4):513-517
Using ELlSA, 83 sera from leprosy patients in National Sorokdo Hospital who are clessified bacteriologically positive and 40 sera from patient's children were tested for a.ntibedy to phenolic glycolipid I of M. leprae. The following results were obtained. I. Non. of 40 contact children was pasitive to PGL- I. 2. Patiente with high bacterial indices were more likely to have antibodies to PGL,- 1. Although the antibody level declined, in general, after multi-drug therapy (MDT), t.here were quite a few with high circulating antibodiees to PGL- I even among the patients bacteriologically below 2.
Antibodies
;
Child*
;
Enzyme-Linked Immunosorbent Assay
;
Humans
;
Leprosy*
;
Mycobacterium leprae*
;
Mycobacterium*
;
Phenol*
3.Lyme disease.
Min Geol LEE ; Kee Yang CHUNG ; Yong CHOI ; Sang Nae CHO
Korean Journal of Dermatology 1993;31(4):601-605
Lyme disease is an infecious disease caused by Borrelia burgdorfieii and has been reported world-wide. The spirochete invades multiple organs and induces manifesations in the skin, joints, neri us and cardiovascular systems. 13espite a considerable amount of serological evidence and isolation of R burgdorferi in ticks, no human case has yet been reported in korea. In this report, we present the first clinical case of Lyme disease in Korea. A female patient, 26 years old, developed clinical signs of eryth m,i chronicum migpans about ten days after traveling to a rural area and responded well to treatment ith entibiotions. Laboratory tests showed elevated IgM and IgG responses to B. burgdorferi, and a spirociete was found in a skin biopsy by electron microscopy.
Adult
;
Biopsy
;
Borrelia
;
Borrelia burgdorferi
;
Cardiovascular System
;
Female
;
Humans
;
Immunoglobulin G
;
Immunoglobulin M
;
Joints
;
Korea
;
Lyme Disease*
;
Microscopy, Electron
;
Skin
;
Spirochaetales
;
Ticks
4.Diversity of Humoral Immune Responses to Recombinant Proteins of Brucella abortus Among Residents in Cheju Province.
Hyung Jin EUH ; Jun Seop YEOM ; Jun Myung KIM ; Joo Deuk KIM ; Sang Nae CHO
Journal of the Korean Society for Microbiology 2000;35(5):377-377
No Abstract Available.
Brucella abortus*
;
Brucella*
;
Immunity, Humoral*
;
Jeju-do*
;
Recombinant Proteins*
5.Prevalence of antibodies to the phase I antigen of coxiella burnetii , the Q fever agent, among residents in Korea.
Sang Nae CHO ; Mi Kyeong LEE ; Jae Myun LEE ; Joo Deuk KIM ; Won Young LEE
Journal of the Korean Society for Microbiology 1992;27(3):283-288
No abstract available.
Antibodies*
;
Coxiella burnetii*
;
Coxiella*
;
Korea*
;
Prevalence*
;
Q Fever*
6.Detection of the anti-neural antibodies in the sera of leprosy patients.
Joo Young PARK ; Jung Koo YOUN ; Sang Nae CHO ; Woo Ick YANG ; Choon Myung KOH
Journal of the Korean Society for Microbiology 1992;27(3):239-251
No abstract available.
Antibodies*
;
Humans
;
Leprosy*
7.Comparison of enzyme-linked immunosorbent assay, indirect immunofluorescent antibody test and indirect immunoperoxidase antibody test in setecting antibodies to rickettsia tsutsugamushi.
Jeon Soo SHIN ; Sang Nae CHO ; Millina LEE ; Yunsop CHONG ; Joo Deuk KIM
Journal of the Korean Society for Microbiology 1991;26(1):79-85
No abstract available.
Antibodies*
;
Enzyme-Linked Immunosorbent Assay*
;
Orientia tsutsugamushi*
;
Rickettsia*
8.Determination of DNA-DNA Hybridization Condition for Rapid Identification of Mycobacterium Species.
Yun Sop CHONG ; Sang Nae CHO ; Kyung Won LEE ; Hong Seok PARK
Journal of the Korean Society for Microbiology 1999;34(2):137-145
Rapid identification of Mycobacterium spp. isolated from patients is important with increased isolation of mycobacteria other than tubercle bacilli (MOTT). DNA-DNA hybridization with streptavidin-peroxidase and tetramethylbenzidine (TMB) color reaction method was recognized as a useful tool for identification of various species of mycobacteria. In this study, optimum condition of the test was determined. The optimal concentrations of tetramethylbenzidine dihydrochloride and hydrogen peroxide for streptavidin-horseradish peroxidase were 0.3-0.6 ug/ ml and 0.16 mM, respectively. The TMB stock solution was stable when prepared in methanol and the dilution of TBM stock solution in 10 mM sodium citrate-10 mM EDTA solution (pH 5.0) gave highest peroxidase-TMB activity. The suitable composition of hybridization solution consisted of 2 x SSC, 10% dextran sulfate, 50 ug/ml salmon DNA, 5 x Denhardt's solution, and 50% formamide. The 5-minute heating at 100C of test DNA prior to photobiotin labeling significantly increased the reaction. In conclusion, DNA-DNA hybridization method with streptavidin-peroxidase and TMB color reaction method may be useful for rapid identification of Mycobacterium spp. isolated from patients.
Dextran Sulfate
;
DNA
;
Edetic Acid
;
Heating
;
Hot Temperature
;
Humans
;
Hydrogen Peroxide
;
Methanol
;
Mycobacterium*
;
Peroxidase
;
Salmon
;
Sodium
9.A Case of Conjoined Twins.
Hyun Joo CHOI ; Eun Sil KIM ; In Sang JEON ; Myung Chul CHO ; Kwang Jeon KIM ; Nae In LIM
Journal of the Korean Pediatric Society 1990;33(11):1562-1566
No abstract available.
Twins, Conjoined*
10.Detection of Mycobacterium leprae in Skin Biopsy Sepcimens From Leprosy patients by Polymerase Chain Reaction.
Kyeong Han YOON ; Sang Nae CHO ; Jung Bok LEE ; Joo Deuk KIM
Korean Journal of Dermatology 1994;32(3):409-415
BACKGROUND: Polymerase chain reaction(PCR) has brought an oppotunity for rapid detection of Mycobacterium leprae in clinical pecimens for the diagnosis of leprosy. Th DNA segment specific to M. leprae was detectable in a matteir of hours and DNA from one orgnisa appeared positive by PCR. However, the PCR tool has not been evaluated using elinical specimeriis from leprosy patients and controls. OBJECTIVE & METHODS: The primers amplifying 372bp segment of rebetitive sequence of M. leprae DNA were used in PCR. Skin biopsy specimens from 102 leprosy patient, and controls were examined for the presence of M. leprae by PCR and the results were aomared with microscopic and histopathologic findings. RESULTS: 1. As a result, of PCR after DNA preparation of M. leprae, six other mycobacteria, ten other bacteria, and skin from leprosy with five other skin biopsy tissues, 372bp DNA fragment was specifically amplified from M. leprae. 2. Dot blot, hybridization of PCR products showed that the 372bp DNA from skin biopsy specimens were derived from M. leprae. 3. As a result of PCR after DNA preparation of 10-fold diluted M. legrae from mouse footpad, PCR gave a positive result as low as one organism. 4. Of 87 specimens in which acid-fast bacilli were found under microcopic examinations 97% had positive PCR results. 5. Of 97 specimens which hadihistopathologic evidences of leprosy 95% had positive PCR results. 6. Of 15 specimens in which acid-fast bacilli were not found under n!icroscopic examinations 73% had positive PCR results. In three of five cases which had neither histopathologic nor microscopic evidences of leprosy had positive PCR results. CONCLUSION: PCR method amplifying 372bp fragment of repetitive seqi,ence was highly sensitive and specific in detecting M. leprae DNA in skin biopsy specimens, thus may be a useful tool as an additive diagnostic method, espcially for cases where microscopic antihystopathologic findings are not definite.
Animals
;
Bacteria
;
Biopsy*
;
Diagnosis
;
DNA
;
Humans
;
Leprosy*
;
Mice
;
Mycobacterium leprae*
;
Mycobacterium*
;
Polymerase Chain Reaction*
;
Skin*