This study was carried out to investigate the morphologic findings and process of castration-induced apoptosis in the rat prostate. The experimental group was treated with bilateral orchiectomy followed by sequential sacrifices at 1, 2, 3, 4, 5, 6, 7 days and 2, 3 weeks (6 rats, respectively). Ventral prostate was extirpated and examined by light microscopic and immunohistochemical, ultrastructural observation. Apoptotic index increased by 4 days after castration and decreased thereafter. ApopTag stain revealed brownish granular pattern in the nucleus of apoptotic cells. DNA fragmentation rate was 0.5% in the control group and began to increase by 1 day after castration and reached to 11.1% by 4 days and decreased thereafter. PCNA stain showed brownish granular pattern in the nucleus of some epithelial cells of the prostatic glands. PCNA labelling index was 2.4% in the control group and reached peak by 3 days after castration and decreased thereafter. Electron microscopically, there was chromatin condensation with margination toward the nuclear membrane by 1 day after castration. Also noted were condensation of cytoplasm, dilatation of RER and nuclear fragmentation. Apoptotic bodies were formed and phagocytosed by adjacent cells and some apoptotic bodies were found in the lumen of acini. Based on these results, it can be concluded that castration-induced prostatic involution is the result of apoptosis. Detection of DNA fragmentation with ApopTag is a more a accurate method to identify not only apoptotic body formation itself but also the previous step of apoptotic body formation. PCNA labelling index to identify the cellular proliferation seems to play an active role in the early step of apoptosis and be a good tool for investigation of apoptosis.
Animals ; Apoptosis* ; Castration ; Cell Proliferation ; Chromatin ; Cytoplasm ; Dilatation ; DNA Fragmentation ; Epithelial Cells ; Nuclear Envelope ; Orchiectomy ; Proliferating Cell Nuclear Antigen ; Prostate* ; Rats*

No Abstract Available.
Anisometropia*

In order to examine a way of utilizing the papers published in the field of oral &maxillofacial surgery, the subjects and the references from total 3,392 papers. ; 263 papers in the KJOMS(Journal of Korean Association of Oral &Maxillofacial Surgeons. vol. 17-21), 237 papers in the KJMPRS(Journal of Korean Academy of Maxillofacial Plastic and Reconstructive Surgery. Vol 13-17), 1,233 papers in the JJOMS (Journal of Japanese Association of Oral &Maxillofacial Surgeons. vol 37-41), 1,212 papers in the AJOMS(Journal of American Association of Oral &Maxillofacial Surgeons. vol 49-53), and 447 papers in the International JOMS(Official publication of the International association of Oral &Maxillofacial Surgeons vol 20-25) have been analyzed. The results were as follows: 1. The subjects of KJOMS and KJMPRS were composed of various 28 items. Among 15 main subjects excluding the minor subjects below 1%, there were Aquired deformity &-Reconstruction (17.4%), Jaw deformity (14.2%), Trauma (14.2%) and Malignant tumor (10.8%). 2. The main subjects of JJOMS were Malignant tumor (22.8%) and Benign tumor (20.6%), In AAOMS, Trauma (13.0%), Jaw deformity (13.0%), and malignant tumor 12.4%) were main subjects. In IJOMS Malignant tumor (16.1%), Acquired deformity &Reconstruction (13.0%), and Trauma (10.3%) were main subjects. 3. IN KJOMS and KJMPRS, Scientific and Clinical articles was 63.5% and Case reports was 36.5%. But scientific and Clinical articles was 43.4% and Case reports was 56.6% in JJOMS, 47.1% and 52.9% in AAOMS, 50.1% and 49.9% in IJOMS. 4. The number of institutes in KJOMS and KJMPRS was 46. The main 6 institute published the 57.4% of total articles. In IJOMS, Nationality of the author was composed of 42 nations. West Europe Area published 52.8% of total articles, and The dominant nation of the author were West Germeny (16.3%), U.K. (12.1%), and Japan (11.6%). 5. Authors cited 30.4 references per articles in average, but only 2.7 domestic articles was cited in KJOMS and KJMPRS. It was 17.4 references, but 9.3 domestic articles was cited. In AJOMS and IJOMS, were 19.5 and 18.0 references.
Academies and Institutes ; Asian Continental Ancestry Group* ; Congenital Abnormalities ; Ethnic Groups ; Europe ; Humans ; Japan ; Jaw ; Plastics ; Publications

No abstract available.

No abstract available.

No abstract available.
Anencephaly* ; Autopsy*

No abstract available.
Pancreas*

No abstract available.
Female ; Mammals* ; Ovulation* ; Rupture*

No abstract available.
Haloperidol* ; Homovanillic Acid* ; Humans ; Plasma*

No abstract available.