1.Experience of therapeutic plasma exchanges in Seoul National University Hospital.
Tae Hyun UM ; Nam Yong LEE ; Hyo Soon PARK ; Kyou Sup HAN ; Sang In KIM
Korean Journal of Blood Transfusion 1993;4(2):199-205
No abstract available.
Plasma Exchange*
;
Plasma*
;
Seoul*
2.Experience of therapeutic plasma exchanges in Seoul National University Hospital.
Tae Hyun UM ; Nam Yong LEE ; Hyo Soon PARK ; Kyou Sup HAN ; Sang In KIM
Korean Journal of Blood Transfusion 1993;4(2):199-205
No abstract available.
Plasma Exchange*
;
Plasma*
;
Seoul*
3.A case of papillary serous carcinoma of the peritoneum.
Young In LEE ; Kee Myoung UM ; Jang Yeon KWON ; Sang Won HAN ; Dong Soo CHA ; Dae Hyun KIM ; Mee Yeon CHO
Korean Journal of Obstetrics and Gynecology 1992;35(10):1537-1543
No abstract available.
Peritoneum*
4.Detection of Platelet-Specific Antibodies Employing Modified Antigen Capture ELISA(MACE).
Tae Hyun UM ; Kyou Sup HAN ; Dae Chul KIM ; Yoo Sung HWANG ; Doo Sung KIM ; Sang In KIM
Korean Journal of Blood Transfusion 1995;6(2):123-130
Widely used tests for the detection of platelet antibodies in Korea include platelet suspension immunofluorescence test(PSIFT), enzyme immunoassay and mixed passive hemagglutination(MPHA). In these tests, removal of HLA antigens from platelet are required to detect platelet-specific antibodies. Modified antigen capture ELISA(MACE) is known to be very sensitive for the detection of platelet-specific antibodies, in which specific platelet glycoprotein, captured by the monoclonal antibody is used as a target antigen. MACE is very useful for the detection of platelet-specific alloantibodies in neonatal alloimmune thrombocytopenia(NAIT) and posttransfusion purpura(PTP). We employed MACE in our laboratory, using AP2(anti-GPIIb/IIIa, monoclonal), #30 sera(anti-PlA1), 90-545 sera(anti-HLA-B51+52) and LYS sera(multispecific HLA antibodies). LYS sera had been used as our positive control( 1:120) in MPHA. Platelet from PIA1(+), HLA-B5 I, blood group O healthy male donor, gave positive result with #30 sera(1:40) and negative result with 90-545 sera in MACE. With LYS sera, MACE showed negative in 1:120, but positive in 1:20. So LYS sera was thought to contain strong multispecific HLA antibodies and relatively weak antibody(-ies) reacting with GPllb/Illa. Further studies employing different monoclonal antibodies, such as anti-GPIb/IX, -GPIa/Ila and -GPIV are under way.
Antibodies*
;
Antibodies, Monoclonal
;
Blood Platelets
;
Fluorescent Antibody Technique
;
Glycoproteins
;
HLA Antigens
;
Humans
;
Immunoenzyme Techniques
;
Isoantibodies
;
Korea
;
Male
;
Tissue Donors
5.A Case of Neonatal Altoimmune Thrombocytopenia due to Anti-HLA-B7 +1=160 +B61.
Kyou Sup HAN ; Tae Hyun UM ; Myoung Hee PARK ; Yong Won PARK ; Bo Moon SHIN ; Sang Woo KIM
Korean Journal of Blood Transfusion 1994;5(1):45-51
We encountered a case of neonatal altoimmune thrombocytopenia(NAIT) due to anti-HLA-B7+B60+B61. Bilateral cephal hematoma and umbilical hematoma were noted at the time of birth. Purpura developed at the third day. Platelet count was 110,000 at birth and decreased to 66,000/micro liter at the day 4. Prothrombin time and partial prothrombin time were within normal limit. The mother's platelet count was 220,000/micro liter and she had no history of abnormal bleeding. Platelet antibody tests empolying mixed passive hemagglutination and immunofluorescence revealed that the mother's serum was reactive against the platelets from the father and the neonate, but was not reactive with her own platelets. Platelets from eight volunteer group 0 donors were tested with the mother's serum; seven were reactive and one was negative. The positive reactions were lost after chloroquine treatment of platelets. Antigen capture ELISA(ACE) and modified antigen capture ELISA employing monoclonal antibodies against platelet glycoproteins In, IIa, IIb, and IIIa were negative. Mother's serum was tested for lymphocytotoxicity against 49 donor ]ymphocytes and the specificity was found to be anti-HLA-B7+B60+B61. At the 9th day, one unit of platelet concentrate from the mother was transfused and the platelet count of the neonate rose up to 340,000/micro liter. The neonate was discharged at the day of sixteenth and the platelet count remained high thereafter.
Antibodies, Monoclonal
;
Blood Platelets
;
Chloroquine
;
Enzyme-Linked Immunosorbent Assay
;
Fathers
;
Fluorescent Antibody Technique
;
Hemagglutination
;
Hematoma
;
Hemorrhage
;
Humans
;
Infant, Newborn
;
Mothers
;
Parturition
;
Platelet Count
;
Platelet Membrane Glycoproteins
;
Prothrombin Time
;
Purpura
;
Sensitivity and Specificity
;
Thrombocytopenia*
;
Tissue Donors
;
Volunteers
6.A Subperiosteal Ganglion of the Distal Radius: A Case Report.
Phil Hyun CHUNG ; Chung Soo HWANG ; Dong Ju CHAE ; Sang Ho MOON ; Dae Jin KIM ; Kyu Hwang UM
The Journal of the Korean Orthopaedic Association 1998;33(3):641-644
A subperiosteal ganglion has been very rarely reported, since Ollier reported first one in 1864. A subperiosteal ganglion is produced by mucoid degeneration and cyst formation within the periosteum, which results in cortical erosion. The characteristic plain radiolographic appearance of irregular cortical erosion and scalloping, with reactive periosteal bone spicules, has been considered pathognomonic of subperiosteal ganglion. Magnetic resonance imaging is performed to further characterize the soft tissue component of the mass. As in our patient, the characteristic signal intensities of magnetic resonance imaging are especially useful in identifying the lesion as a ganglion and in defining the lobular nature and the anatomical extent. We report here the case of a 35-year-old female who had a sudperiosteal ganglion of the distal radius.
Adult
;
Female
;
Ganglion Cysts*
;
Humans
;
Magnetic Resonance Imaging
;
Pectinidae
;
Periosteum
;
Radius*
7.Associations of COL2A1 Gene Polymorphisms and Ankylosing Spondylitis in the Korean Population
Eun-Seok SON ; Sang-Hyun UM ; Moon-Yul YOUN
Journal of Korean Society of Spine Surgery 2020;27(1):1-8
Objectives:
The aim of this study was to investigate whether single nucleotide polymorphisms (SNPs) of COL2A1 affect the development of ankylosing spondylitis (AS).Summary of Literature Review: Many factors have been reported to be involved in the etiology of AS. Human leukocyte antigen (HLA)-B27 has been established as a genetic factor involved in the development of AS; however, it has been reported in recent studies that various genetic polymorphisms may be related to the development of AS. The collagen, type II, alpha 1 gene (COL2A1 ) plays a role in cartilage formation and maintaining the vitreous humor in the eye. Several previous studies have investigated the associations of COL2A1 with spinal degenerative diseases, but no case-control comparative study has yet investigated the effect of COL2A1 variants on the development of AS.
Materials and Methods:
The study was planned with 96 AS patients in the study group and 330 healthy individuals in the control group. We searched the gene region of the COL2A1 gene in the NCBI SNP database (http://www.ncbi.nlm.nih.gov/snp), and 3 SNPs (rs3803183, rs2070739 and rs1793949) were found using sequencing to be significantly different between the AS and control groups. Multiple logistic regression models for genetic analysis were applied
Results:
Three SNPs (rs3803183, rs2070739 and rs1793949) of COL2A1 showed significant associations with AS patients compared to control subjects (p<0.05).
Conclusions
SNPs of COL2A1 may be associated with the development of AS in the Korean population.
8.Diagnostic Validity of an Automated Probabilistic Tractography in Amnestic Mild Cognitive Impairment.
Won Sang JUNG ; Yoo Hyun UM ; Dong Woo KANG ; Chang Uk LEE ; Young Sup WOO ; Won Myong BAHK ; Hyun Kook LIM
Clinical Psychopharmacology and Neuroscience 2018;16(2):144-152
OBJECTIVE: Although several prior works showed the white matter (WM) integrity changes in amnestic mild cognitive impairment (aMCI) and Alzheimer's disease, it is still unclear the diagnostic accuracy of the WM integrity measurements using diffusion tensor imaging (DTI) in discriminating aMCI from normal controls. The aim of this study is to explore diagnostic validity of whole brain automated probabilistic tractography in discriminating aMCI from normal controls. METHODS: One hundred-two subjects (50 aMCI and 52 normal controls) were included and underwent DTI scans. Whole brain WM tracts were reconstructed with automated probabilistic tractography. Fractional anisotropy (FA) and mean diffusivity (MD) values of the memory related WM tracts were measured and compared between the aMCI and the normal control groups. In addition, the diagnostic validities of these WM tracts were evaluated. RESULTS: Decreased FA and increased MD values of memory related WM tracts were observed in the aMCI group compared with the control group. Among FA and MD value of each tract, the FA value of left cingulum angular bundle showed the highest area under the curve (AUC) of 0.85 with a sensitivity of 88.2%, a specificity of 76.9% in differentiating MCI patients from control subjects. Furthermore, the combination FA values of WM integrity measures of memory related WM tracts showed AUC value of 0.98, a sensitivity of 96%, a specificity of 94.2%. CONCLUSION: Our results with good diagnostic validity of WM integrity measurements suggest DTI might be promising neuroimaging tool for early detection of aMCI and AD patients.
Alzheimer Disease
;
Anisotropy
;
Area Under Curve
;
Brain
;
Diffusion Tensor Imaging
;
Humans
;
Memory
;
Mild Cognitive Impairment*
;
Neuroimaging
;
Sensitivity and Specificity
;
White Matter
9.Successful Treatment with Empirical Erlotinib in a Patient with Respiratory Failure Caused by Extensive Lung Adenocarcinoma.
Suk Hyeon JEONG ; Sang Won UM ; Hyun LEE ; Kyeongman JEON ; Kyung Jong LEE ; Gee Young SUH ; Man Pyo CHUNG ; Hojoong KIM ; O Jung KWON ; Yoon La CHOI
Korean Journal of Critical Care Medicine 2016;31(1):44-48
We herein describe a 70-year-old woman who presented with respiratory failure due to extensive lung adenocarcinoma. Despite advanced disease, care in the intensive care unit with ventilator support was performed because she was a newly diagnosed patient and was considered to have the potential to recover after cancer treatment. Because prompt control of the cancer was needed to treat the respiratory failure, empirical treatment with an oral epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor was initiated before confirmation of EGFR-mutant adenocarcinoma, and the patient was successfully treated. Later, EGFR-mutant adenocarcinoma was confirmed.
Adenocarcinoma*
;
Aged
;
Female
;
Humans
;
Intensive Care Units
;
Lung*
;
Protein-Tyrosine Kinases
;
Receptor, Epidermal Growth Factor
;
Respiration, Artificial
;
Respiratory Insufficiency*
;
Ventilators, Mechanical
;
Erlotinib Hydrochloride
10.Demineralized dentin matrix combined with recombinant human bone morphogenetic protein-2 in rabbit calvarial defects.
In Woong UM ; Suk Hyun HWANG ; Young Kyun KIM ; Moon Young KIM ; Sang Ho JUN ; Jae Jun RYU ; Hyon Seok JANG
Journal of the Korean Association of Oral and Maxillofacial Surgeons 2016;42(2):90-98
OBJECTIVES: The aim of this study was to compare the osteogenic effects of demineralized dentin matrix (DDM) combined with recombinant human bone morphogenetic protein-2 (rhBMP-2) in rabbit calvarial defects with DDM and anorganic bovine bone (ABB) combined with rhBMP-2. MATERIALS AND METHODS: Four round defects with 8-mm diameters were created in each rabbit calvaria. Each defect was treated with one of the following: 1) DDM, 2) ABB/rhBMP-2, or 3) DDM/rhBMP-2. The rhBMP-2 was combined with DDM and ABB according to a stepwise dry and dip lyophilizing protocol. Histological and microcomputed tomography (µCT) analyses were performed to measure the amount of bone formation and bone volume after 2- and 8-week healing intervals. RESULTS: Upon histological observation at two weeks, the DDM and ABB/rhBMP-2 groups showed osteoconductive bone formation, while the DDM/rhBMP-2 group showed osteoconductive and osteoinductive bone formation. New bone formation was higher in DDM/rhBMP-2, DDM and ABB decreasing order. The amounts of bone formation were very similar at two weeks; however, at eight weeks, the DDM/rhBMP-2 group showed a two-fold greater amount of bone formation compared to the DDM and ABB/rhBMP-2 groups. The µCT analysis showed markedly increased bone volume in the DDM/rhBMP-2 group at eight weeks compared with that of the DDM group. Notably, there was a slight decrease in bone volume in the ABB/rhBMP-2 group at eight weeks. There were no significant differences among the DDM, ABB/rhBMP-2, and DDM/rhBMP-2 groups at two or eight weeks. CONCLUSION: Within the limitations of this study, DDM appears to be a suitable carrier for rhBMP-2 in orthotopic sites.
Dentin*
;
Humans*
;
Osteogenesis
;
Skull
;
X-Ray Microtomography