No abstract available.
Female ; Follicle Stimulating Hormone* ; Gonadotropin-Releasing Hormone* ; Gonadotropins* ; Humans ; Ovarian Diseases*

No abstract available.

No abstract available.
Female ; Methotrexate* ; Pregnancy ; Pregnancy, Tubal*

No abstract available.
Contraceptives, Oral* ; Fertilization in Vitro* ; Gonadotropins*

No abstract available.
Embryo Transfer* ; Embryonic Structures* ; Fertilization in Vitro* ; Pregnancy*

OBJECTIVE: Recently, we have demonstrated that embryonic ventral spinal cord motor neurons(ESMNs), transplanted into the distal stump of the axotomized tibial nerve, can grow into the denervated gastrocnemius muscle and form neuromuscular junctions. Our interest in study was to see whether these newly formed neuronal connections are physiologically active, and to electrophysiologically characterize the reinnervated motor units. MATERIAL AND METHODS: Three to eight weeks after transplantation rats were prepared for electrophysiological recording. Motor unit (MU) action potential and gross EMG were monitored. In eleven out of sixteen transplanted animals single unit and gross EMG were recorded after electrical stimulation of the transplant site. Total of 63 motor units were analyzed for their stimulus threshold (ST), latency and stimulus intensity, which is required to produce maximum firing. RESULTS: ST intensities activating MUs were significantly higher in transplanted animals than in controls. Maximum firing of MUs occurred at less than 1.2×T in the control but greater variation was observed in the transplanted animals ranging from 1.1×T to 1.6×T. No significant differences were found in the latencies of MU's firing following stimulation. MU firing was reversibly blocked by infusion of succinylcholine. CONCLUSION: we characterized the temporal, morphological and electrophysiological parameters of denervated skeletal muscle reinnervated by dissociated grafts of embryonic ventral spinal cord cells. MUs reinnervated by the embryonic motoneuronal transplant were physiologically active, with some properties similar to the MUs of the normal.
Action Potentials ; Animals ; Electric Stimulation ; Fires ; Mice ; Motor Neurons ; Muscle, Skeletal ; Neuromuscular Junction ; Neurons ; Rats ; Spinal Cord ; Succinylcholine ; Tibial Nerve ; Transplants

No abstract available.
Fertilization in Vitro* ; Gonadotropin-Releasing Hormone*

No abstract available.
Electroconvulsive Therapy ; Lacerations ; Tongue

To investigate the effects of prostaglandin E1(PGX1) in prevention of proliferative scar formation, we cultured fibroblasts of normal skin (NS), hypertrophic scar (HS) and keloid (KL) tissues obtained from patients. We have compared type I collagenase production of cultured fibroblasts from normal skin, hypertrophic scar, and keloid tissues under various concentrations of PGE1. Our results demonstrate that type I collagenase production was significantly increased after addition of PGE1 in HS and KL, but not NS. Type I collagenase production of HS and KL fibroblasts were increased similarly in 10M and 10M of PGE1 and maximally increased in the concentration of 10M. This promotive effects of PGE1 on the production of type I collagenase was larger in KL than in HS. These results also suggest that PGE1 may play the promotive effects on type I collagenase production in dose-dependent manner. PGE1 may have a role in the prevention of hypertrophic scar and keloid by enhancing the production of type I collagenase of HS and KL fibroblasts. The promotive effects of PGE1 on type I collagenase production was variable depending on its concentration, and its effects was maximum in certain optimal condition. The maximally effective concentration of PGE1 in the prevention of proliferative scar formation should be searched in further investigations for clinical use.
Alprostadil ; Cicatrix ; Cicatrix, Hypertrophic* ; Collagenases* ; Fibroblasts* ; Humans ; Keloid* ; RNA, Messenger* ; Skin

The depth from the skin to the thoracic epidural space was measured in 121 patients (41 male and 80 female patients) receiving epidural analgesia. The patients age, sex, height, weight were obtained before measuring the distance. The needle angle to the vertical axis to the skin was obtained during inserting Touhy needle. The mean depth was 6.0+/- 1.3 cm, which correlated with other anatomic measurements except height. The depth was correlated with the needle angle. The group with needle angle greater than 30(0) was greater in depth than the other groups. The mean depth was 6.0+/-1.4 cm in males and 6.0+/-1.3 cm in females and there was no difference in depth between male and female. The mean depth was 5.7+/-1.3 cm at the T5-6 interspace and 6.2+/-1.3 cm at the T6-7 interspace and there was no difference in depth between the two groups.
Analgesia, Epidural ; Axis, Cervical Vertebra ; Epidural Space* ; Female ; Humans ; Male ; Needles ; Skin*