1.Effects of amino acids and some drugs related to gaba receptors on the neuronal activity in isolated medullary dorsal horn of rat.
Journal of the Korean Association of Oral and Maxillofacial Surgeons 1993;19(3):401-411
No abstract available.
Amino Acids*
;
Animals
;
gamma-Aminobutyric Acid*
;
Horns*
;
Neurons*
;
Rats*
;
Receptors, GABA*
2.Effects of Thera-Band(R) Resistance Training Including Isometric Exercise in Total Knee Replacement Patients.
Youn Jung KIM ; Mi Nyeo YOON ; Sang Sook HAN
Korean Journal of Health Promotion 2011;11(2):82-90
BACKGROUND: The purpose of this study was to assess the effects of an exercise program using isometric and isotonic thera band exercises. METHODS: Data were collected from 30 total knee replacement patients hospitalized in the orthopedics department at 'B' hospital (control=15, experimental=15). The control group performed isometric exercises for one week while the experimental group performed three or more rounds of both isometric and isotonic exercises, including Thera-Band(R) exercises, everyday for four weeks. RESULTS: The experimental group improved significantly in muscular strength (Z=-1.99, P=0.047) and self-efficacy (Z=19.48, P=0.000), and showed lower levels of depression than the control group (Z=8.60, P=0.003). However, no difference was found in pain experience between the two groups. CONCLUSIONS: Exercise programs using the isometric and Thera-Band(R) resistance training improve muscular strength and self-efficacy and relieve depression in total knee replacement patients.
Arthroplasty, Replacement, Knee
;
Depression
;
Exercise
;
Humans
;
Orthopedics
;
Resistance Training
3.Rapid Diagnosis of Isoniazid Resistance by Detection of Mutations in katG and inhA of Mycobacterium tuberculosis from Korea.
Sang Jae KIM ; Seok Yong KIM ; Ji Youn LEE ; Sang Ryeol RYU ; Gil Han BAI
Journal of the Korean Society for Microbiology 1997;32(5):569-576
29 isoniazid (INH) resistant isolated strains and INH sensitive reference strain (H37Rv) of Mycobacterium tuberculosis were analysed by polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) and NciI restriction mapping for the detection of mutations in katG gene and inhA gene. The katG gene was divided into 3 parts (Akat, Bkat, Ckat; each part is about 800 bp) and amplified, inhA gene was amplified as a whole. Each of the amplified 800 bp DNA was digested into small fragments of less than 400 bp with restriction enzymes for the direct PCR-SSCP analysis. Firstly, 10 strains were analysed. All the 10 isolates showed clearly distinct SSCP patterns in Bkat from that of the reference strain, but only two isolates showed distinct SSCP patterns in Akat, and no isolated strain showed any distinct SSCP patterns in Ckat. 10 isolates also showed distinct SSCP patterns in inhA. NciI restriction mapping of Bkat showed mutation in codon 463 in 7 strains among 10 isolated strains. With these results an early detection strategy for the INH resistant M. tuberculosis was applied to the rest of 19 isolated INH resistant strains. Firstly, isolates were screened by Ncsl mapping in Bkat, and 13 strains showed mutations in codon 463. Secondly, the rest of 6 INH resistant isolates were analysed by PCR-SSCP with restriction enzyme digestion (PCR-SSCP-RE) in Bkat, and all the strains showed distinct SSCP patterns from that of the INH sensitive reference strain. This proved our strategy as effective and economic and time saving method in early detection of INH resistant M. tuberculosis.
Codon
;
Diagnosis*
;
Digestion
;
DNA
;
Isoniazid*
;
Korea*
;
Mycobacterium tuberculosis*
;
Mycobacterium*
;
Polymorphism, Single-Stranded Conformational
;
Restriction Mapping
;
Tuberculosis
4.Detection of canine distemper virus (CDV) through one step RT-PCR combined with nested PCR.
Yong Hwan KIM ; Kyu Woan CHO ; Hwa Young YOUN ; Han Sang YOO ; Hong Ryul HAN
Journal of Veterinary Science 2001;2(1):59-63
A one step reverse transcription PCR (RT-PCR) combined nested PCR was set up to increase efficiency in the diagnosis of canine distemper virus (CDV) infection after developement of nested PCR. Two PCR primer sets were designed based on the sequence of nucleocapsid gene of CDV Onderstepoort strain. One-step RT-PCR with the outer primer pair was revealed to detect 10(2) PFU/ml. The sensitivity was increased hundredfold using the one-step RT-PCR combined with the nested PCR. Specificity of the PCR was also confirmed using other related canine virus and peripheral blood mononuclear cells (PBMC) and body secretes of healthy dogs. Of the 51 blood samples from dogs clinically suspected of CD, 45 samples were revealed as positive by one-step RT-PCR combined with nested PCR. However, only 15 samples were identified as positive with a single one step RT-PCR. Therefore approximately 60% increase in the efficiency of the diagnosis was observed by the combined method. These results suggested that one step RT-PCR combined with nested PCR could be a sensitive, specific, and practical method for diagnosis of CDV infection.
Animals
;
Distemper Virus, Canine/genetics/*isolation & purification
;
Dogs
;
Polymerase Chain Reaction/*methods/*veterinary
;
RNA, Viral/genetics/isolation & purification
;
Reverse Transcriptase Polymerase Chain Reaction
;
Vaccines, Attenuated
;
Viral Vaccines
5.Effect of obesity and age on the blood pressure.
Youn Jin KIM ; Young Hoon HAN ; Sang Wook PARK ; Byung Mann CHO ; Hyung Su SEOL ; Youn Jeong HEO
Journal of the Korean Academy of Family Medicine 1997;18(3):295-305
BACKGROUND: In recent years, the incidence of obesity increased in Koreans. The main cause suggested is the diet style of Korean changed to that of Western. It has been proved that obesity is a risk factor or an aggravating factor of hypertension and diabetes mellitus, cardiovascular disease, etc. Primary physician has been interested in the correlation of blood pressure with relative body weight and aging. The purpose of this study was to focus on the influence of age and relative body weight on blood pressure. METHODS: Subjects were collected from 2,068 adults who had received Adult Health Examination. That subjects were divided according to age, relative body weight and then the relation of blood pressure with age according to relative body weight and relation with relative body weight according to age were investigated. RESULTS: Systolic blood pressure increased significantly according to age in all male and female three weight groups and then increased significantly according to relative body weight in all male and female three age groups except male seventh decade group. Diastolic blood pressure increased significantly according to age in all male and female three weight groups except male obesity group and then increased significantly according to the relative body weight in all male and female three age groups except male and female seventh decade groups. Systolic blood pressure has higher correlation with age than relative body weight and diastolic blood pressure has higher correlation with relative body weight in male. In female, systolic and diastolic blood pressure have higher correlations with age. CONCLUSIONS: It can be suggested that blood pressure increase significantly according to aging process and relative body weight. These two factors have a little different effects to systolic and diastolic blood pressure according to sex. Relative body weight has more effect to diastolic blood pressure than systolic blood pressure in male.
Adult
;
Aging
;
Blood Pressure*
;
Body Weight
;
Cardiovascular Diseases
;
Diabetes Mellitus
;
Diet
;
Female
;
Humans
;
Hypertension
;
Incidence
;
Male
;
Obesity*
;
Risk Factors
6.Two cases of renal vein thrombosis in patients with systemic lupus erythematosus.
Sang Heon LEE ; Young Shin SHIN ; Ji Youn HAN ; Jae Kyung CHOI ; Seung Hun LEE ; Youn Sik HONG ; Chul Soo CHO ; Dong Jun PARK ; Ho Youn KIM
Korean Journal of Medicine 1993;45(3):376-382
No abstract available.
Humans
;
Lupus Erythematosus, Systemic*
;
Renal Veins*
;
Thrombosis*
7.The effects of UVB and UVA on the scheduled and unscheduled DNA synthesis of mouse epidermal cells.
Kyu Han KIM ; Bang Soon KIM ; Sang Eun MOON ; Jai Il YOUN
Korean Journal of Dermatology 1992;30(2):186-196
After UV irradiation to mouse epidermis, autoradiography using H-thymidine was performed to study the effects of UV on scheduled and unschcdu ed DNA syntheses. The results can be summarized as follows. l. In case of UVB 100mJ/cm, heavily labeled cells (HLCs) representing scheduled DNA synthesis began to decrease immediately after UVB irradiai,ior and were significantly decreased after 24 hours, but then recovered after 48 hours. The ecovery was maintained from 72 hours, up to 7 days. Sparsely labeled cells (SLCs) repres inting unscheduled DNA synthesis were significantly increased from immediately after UV 3 irradiation and up to 6 hours later. Repair was present at 24 hours, and was maintained at 48 hours, 72 hours, and 7 day after irradiation. 2. In case of UVA, UVA 10J/cm did not affect DNA synthesis sign ficantly. But in cases of UVA 30J/cm and 50J/cm, HLCs began to decrease immediately after UVA irradiation and were significantly decreased for 24 hours. Recovery occured at 48 hours, and was maintained from 72 hours up to 7 days. SLCs were significently increased immediately after and up to 6 hours after UVB irradiation. Repair generally occurred after 24 hours, and was maintained at 48 hours, 72 hours, and 7 days after ir rad ation.
Animals
;
Autoradiography
;
DNA*
;
Epidermis
;
Mice*
8.A Rapid and Simple flow Cytometric Method for Measuring Cell Viability Using Propidium Iodide Staining and Forward Scatter Measurement.
Yong Suk LEE ; Sang Woong YOUN ; Kyu Han KIM ; Kyoung Chan PARK
Annals of Dermatology 1996;8(3):195-200
BACKGROUND: The importance of the determination of cell viability has prompted the development of several assays of viability that utilize the exclusion of certain dyes by viable cell membranes. Recently, flow cytometry has been adapted to estimate cell viability by using fluorescent dye which is excluded by living cells on the basis of altered dead cell properties. OBJECTIVE: We have developed a flow Cytometric method for measuring cell viability after staining with propidium iodide (PI) and have compared it with the classical colorimetric method, MTT assay, which is currently widely used in cytotoxicity assays in the research field. METHODS: We performed flow cytometry and MTT assay for the comparison of the sensitivity of the assessment of cell viability. RESULTS: Decrease of cell viability was measured by flow cytometry with the addition of as little as 0.002% Triton-X 100 in comparison to MTT assay which could only reveal a similar decrease of cell viability with the new method to 0.008% Triton-X 100. CONCLUSION: Our results demonstrate this new method to be more sensitive and simple for the assessment of cell viability.
Cell Membrane
;
Cell Survival*
;
Coloring Agents
;
Flow Cytometry
;
Methods*
;
Propidium*
9.A Case of Hallermann-Streiff Syndrome.
Jin Sang SON ; Ho Youn HWANG ; Han Ku MOON ; Jeong Ok HAH
Journal of the Korean Pediatric Society 1987;30(6):691-694
No abstract available.
Hallermann's Syndrome*
10.Papillary Serous Cystadenoma of Borderline Malignancy Arising from a Paramesonephric Parovarian Cyst: A case report.
Ji Han JUNG ; Youn Soo LEE ; Seok Jin KANG ; Byung Kee KIM ; Sang In SHIM
Korean Journal of Pathology 1999;33(6):457-459
Primary malignant parovarian epithelial tumors are extremely rare, with only 56 cases previously reported in the world literature. Most parovarian epithelial tumors are of paramesonephric (Mullerian) origin. In this article, we report the first case in the Korean literature of papillary serous cystadenoma of borderline malignancy from paramesonephric parovarian cyst. This case presented here is of particular interest since this neoplasm is rare. A 48-year-old female underwent a hysterectomy with bilateral adnexectomy and pelvic lymph node dissection for a cystic tumor of the right parovarian area. The tumor, measuring 13 cm in diameter, was apart from the ovaries, fallopian tubes and uterus. The cyst wall had well-developed smooth muscle layers. The cyst was lined with mildly atypical ciliated and non-ciliated low columnar cells compatible with those of paramesonephric origin. From the inner surface of the cyst several cauliflower-like structures projected into the lumen. They were of a predominantly papillary architecture covered by atypical epithelial cells with piling-up and occasional glandular growth. No destructive stromal invasion was identified.
Cystadenoma, Serous*
;
Epithelial Cells
;
Fallopian Tubes
;
Female
;
Humans
;
Hysterectomy
;
Lymph Node Excision
;
Middle Aged
;
Muscle, Smooth
;
Ovary
;
Parovarian Cyst*
;
Uterus