No abstract available.
Popliteal Artery

PURPOSE: Because cryptorchid testes are known to undergo histopathologic changes affecting development, maturation, and tertility, early surgical correction is usually recommended. However there are differing opinions concerning whether retractile testes are affected by similar changes and also whether there is a need for surgical treatment. We aimed to assess the histopathologic changes in retractile testis by studying the changes in testes artificially placed back in the abdomen after they have descended to scrotum in experimental rat models. MATERIALS AND METHODS: Male Sprague-Dawley rats were divided into 3 groups; a control group(controls), prepubertal ascent group (P) in which testis were placed back intraabdominally by bilateral inguinal canal obstruction at 6 weeks, a neonatal group(N) in which intraabdminal testis was induced by bilateral inguinal canal obstruction at birth. The relative weight of the testis, morphology of the seminiferous tubules including Leydig cells, tubular degeneration phase(TDP), spermatogonia per tubules(S/T), and Sertoli cell index (SCI) of these three groups were analyzed and compared. RESULTS: The relative weight of testis was significantly decreased in the group of N(0.0016+/-0.001) and P(0.0015+/-0.0002) compared to controls(0.0037+/-0.0002) (p<0.05). The S/T value was also decreased in P(2.05+/-18.2) and N(73.2+/-32.4) when compared to controls (360.2+/-21.3). Similar changes were observed in SCI of both P(64.5+/-6.4) and N (91.2+/-14.2) when compared to controls (227.9+/-31.1). Only minority of N and P showed higher TDP values. However, although statistically insignificant, TDP was increased in both P and N when compared to controls. The Leydig cells in N and P showed cellular distortion and hypertrophy. CONCLUSIONS: This study demonstrate that prepubertal ascent, similar to that of innate cryptorchid testis, also can induce histopathologic changes such as changes in testicular seminiferous tubule, decrease in the S/T value and decrease in SCI value. Our findings supports that hypothesis that retractile testis may cause histological damage thus surgical correction may also be warranted similar to in case of genuine cryptorchism.
Abdomen* ; Animals ; Cryptorchidism ; Humans ; Hypertrophy ; Inguinal Canal ; Leydig Cells ; Male ; Models, Animal ; Models, Theoretical ; Parturition ; Rats* ; Rats, Sprague-Dawley ; Scrotum ; Seminiferous Tubules ; Spermatogonia ; Testis*

A patient had total occlusion of the left main coronary artery that was proved by coronary arteriography. Patients with total occlusion of the left main coronary artery have a varying clinical presentation and may have prolonged survival. In patients with good collaterals, left ventricular function may be preserved. This report reveiws the clinical and angiographic findings of a patient with occlusion of the left main coronary artery with symptoms of unstable angina pectoris but without congestive heart failure or EKG evidence of myocardial infarction.
Angina, Unstable ; Angiography ; Coronary Vessels* ; Electrocardiography ; Heart Failure ; Humans ; Myocardial Infarction ; Ventricular Function, Left

BACKGROUND: The importance of the determination of cell viability has prompted the development of several assays of viability that utilize the exclusion of certain dyes by viable cell membranes. Recently, flow cytometry has been adapted to estimate cell viability by using fluorescent dye which is excluded by living cells on the basis of altered dead cell properties. OBJECTIVE: We have developed a flow Cytometric method for measuring cell viability after staining with propidium iodide (PI) and have compared it with the classical colorimetric method, MTT assay, which is currently widely used in cytotoxicity assays in the research field. METHODS: We performed flow cytometry and MTT assay for the comparison of the sensitivity of the assessment of cell viability. RESULTS: Decrease of cell viability was measured by flow cytometry with the addition of as little as 0.002% Triton-X 100 in comparison to MTT assay which could only reveal a similar decrease of cell viability with the new method to 0.008% Triton-X 100. CONCLUSION: Our results demonstrate this new method to be more sensitive and simple for the assessment of cell viability.
Cell Membrane ; Cell Survival* ; Coloring Agents ; Flow Cytometry ; Methods* ; Propidium*

A gene p53, which is a tumor suppressor gene located on chromosome 17p, regulates cell growth and proliferation, and inactivation of this gene appears to be a common abnormality in many malignant tumors in human. To investigate the prevalence and patterns of p53 protein overexpression and the significance of the p53 protein overexpression as a marker for poor prognosis in transitional cell carcinoma of the bladder which were treated with radical cystectomy, fifty deparaffinized archival specimens from radical cystectomy for transitional cell carcinoma of the bladder were analyzed immunohistochemically to detect overexpression of p53 protein using the mouse monoclonal antibody DO-7 with a standard avidin-biotin immunoperoxidase method. The incidence of p53 expression was 17 of50 (34 per cent) specimens. The p53 expression was associated with histopathologic grade but not with T stage and the status of lymph node involvement. There was no evidence of high recurrence rate, metastasis rate or low survival rate in patients with p53 positive group compared to p53 negative group. But the patients with p53 positive group showed higher T stage and shorter mean survival time than those with p53 negative group. It was not statistically significant. Among many variables evaluated with multivariate analysis, T stage was a significant factor in terms of metastasis, while the status of lymph node involvement was a significant one in terms of survival. In conclusion, the incidence of p53 gene mutation appeared to be higher in invasive and high grade bladder cancer than in superficial and low grade ones. The p53 expression failed to show an unfavorable prognostic factor in transitional cell carcinoma of the bladder treated with radical cystectomy. Prospective studies of large cohorts of specimens are needed to evaluate nuclear overexpression of p53 protein as a prognostic marker in bladder cancer.
Animals ; Carcinoma, Transitional Cell* ; Cohort Studies ; Cystectomy* ; Genes, p53 ; Genes, Tumor Suppressor ; Genes, vif ; Humans ; Incidence ; Lymph Nodes ; Mice ; Multivariate Analysis ; Neoplasm Metastasis ; Prevalence ; Prognosis ; Recurrence ; Staphylococcal Protein A ; Survival Rate ; Urinary Bladder Neoplasms ; Urinary Bladder*

No abstract available.
Neoplasm Metastasis*

The prognosis of infectious endophthalmitis has improved considerably in recent years with refinements in early diagnosis, proper antibiotic therapy and vitreous surgery. But the disease is still potentially catastrophic in spite of proper intervention. The purpose of this study was to evaluate the effect of continuous intravitreal irrigation(lavage) after vitrectomy and lensectomy in intractable endophthalmitis. We injected cultured Pseudomonas(1.5 X 10(5)organism/0.1ml) into the anterior chamber or vitreous cavity of 11 rabbits. In both experimental group(8 eyes) and control group(3 eyes), vitrectomy and lensectomy was done 12 hours after inoculation. In the experimental group, silicone tube was installed thru pars plana sclerotomy site as an infusion and drainage port. Vitreous cavity was irrigated for 3 days with normal saline(1000 ml/day) mixed with amikacin (10 mg/day) and dexamethasone(60 mg/day) via this silicone tube. In the control group, we injected 0.1 ml amikacin(100 microgram/0.1 ml) into vitreous cavity at the end of operation and on daily basis for 3 days. Both groups underwent subconjunctival(25 mg/day) and intramuscular (25 mg/kg/day) injection of amikacin for postoperative 3 days. Electroretinograms of two experimental eyes were taken to evaluate retinal toxicity. On postoperative 7th day, all eyes were enucleated to evaluate inflammatory and toxic changes with light microscope. Electroretinograms of experimental eyes became extinguished on the 7th postoperative day. But we observed that the experimental group showed less inflammatory change than control group in the vitreous cavity and retina.
Amikacin ; Anterior Chamber ; Dexamethasone ; Drainage ; Early Diagnosis ; Endophthalmitis* ; Prognosis ; Rabbits ; Retina ; Retinaldehyde ; Silicones ; Vitrectomy

The subretinal neovascularization(SRN) is the leading cause of blindness in various retinal degenerative disease. Yet the mechanism of subretinal new vessel formation is not fully known. We induced SRN using laser photocoagulation in the rabbit and investigated the efficacy of various combinations of laser parameters. In the present study, eighteen eyes of nine pigmented rabbits underwent argon laser photocoagulation, with different settings of power, spot size, and exposure time, Fluorescein angiography was performed 1, 3 and 8 wks after photocoagulation to evaluate the development of SRN, and the light microscopic and electron microscopic examination was done to verify it at the 3rd and 8th week. The mean incidence of SRN was 48.6%, SRN developed with the highest incidence(67%) when using the setting of 1000mW, 0.2 sec, 250 micrometer (P<0.01). Intraoperative retinal hemorrhage increased the incidence of SRN up to 76.4%(P<0.05). Our result provides the most efficient laser parameters to induce experimental SRN in the pigmented rabbits.
Argon* ; Blindness ; Fluorescein Angiography ; Incidence ; Light Coagulation ; Rabbits* ; Retinal Hemorrhage ; Retinaldehyde

No abstract available.
Graves Disease* ; Insulin*

In order to examine the morphology and ultrastructure of contaminated soft contact lens by scanning electron microscopy, soft contact lenses were contaminated with P.aeruginosa, S.aureus, S.epidermidis and C .albicans. After incubation of soft contact lens in P.aeruginosa suspension for 60 minutes, a number of P.aeruginosa, S.aureus and S.epidermidis were attached to soft contact lens and amorphous materials covering the some of the soft contact lens surface were observed. Also, C.albicans attached to soft contact lens, looking like healthy and the fine fibrillar material covered the surface of soft contact lens and interconnected between cell walls of C.albicans at 14 days after contamination. Soft contact lens may provide a suitable environment for microorganisms that they could attach and proliferate. And microoragisms themselves secreted amorphous materials to protect them from the outside and promote the proliferation. So, contaminated soft contact lens may play a role as pathogen to the cornea.
Candida albicans ; Cell Wall ; Contact Lenses, Hydrophilic* ; Cornea ; Microscopy, Electron, Scanning ; Pseudomonas aeruginosa ; Staphylococcus aureus ; Staphylococcus epidermidis