OBJECTIVE: Flow cytometric deoxyribonucleic acid (DNA) content analysis has been shown to be of prognostic importance in some cancers. In this study, DNA patterns obtained by flow cytometry in patients with cervical cancer have been shown to be related to a prognostic importance in cervical carcinoma. METHODS AND MATERIALS: We retrospectively reviewed 59 cervical cancer patients who admitted to the department of obstetrics and gynecology, Samsung Medical Center firom January 1995 to May 1997. RESULTS: A total of 59 archival paraffin-embedded blocks were proc 42.4 percent were found to be DNA content aneuploid (DNA-A) and 57.6 percent DNA content diploid (DNA-D). The S-phase fraction was significantly increased with stage. The incidences of aneuploid and relative DNA index were not significantly increased with the stage(P=0.280) and age of patients(P=0.088). The results had no significant differences between the squamous cell carcinoma and adenocarcinoma, and no relationship between the groups with keratinizing cell type and nonkeratinizing type(P=0.409). The DNA ploidy patterns were not significantly correlated with metastasis to lymph node(P=0.179), tumor size(P=0.109), invasion depth(P=0.361) and recurrent group(P=0.285). High S-phase fraction had a tendency to correlate with the invasion depth(P=0,011), size(P=0.027), lymph node metastasis(P=0.039) and recurence(P=0.099) of cervical cancer. CONCLUSION: Flow cytometric DNA content analysis is being used more frequently in the management of different malignant tumors. Our study shows that DNA content analysis may not serve as an important biological indicator in determining the prognosis in cervical carcinoma, but High S-phase fraction has been connected with more aggressive tumors.
Adenocarcinoma ; Aneuploidy ; Carcinoma, Squamous Cell ; Cervix Uteri* ; Diploidy ; DNA* ; Female ; Flow Cytometry ; Gynecology ; Humans ; Incidence ; Lymph Nodes ; Neoplasm Metastasis ; Obstetrics ; Ploidies ; Prognosis ; Retrospective Studies ; Uterine Cervical Neoplasms

A case of anencephaly associatied with twin pregnancy that delivered by primary lo-wer flap transverse cesarean section at 35-weeks of gestation is presented with brief revi-ew of literatures.
Anencephaly* ; Cesarean Section ; Female ; Humans ; Pregnancy ; Pregnancy, Twin*

The aim of this study was to measure the cusp deflection during composite restoration for MOD cavity in premolar and to examine the influence of cavity dimension, C-factor and restoration method on the cusp deflection. Thirty extracted maxillary premolar were prepared to four different sizes of MOD cavity and divided into six groups. The width and depth of the cavity were as follows. Group 1; 1.5 x 1 mm, Group 2; 1.5 x 2 mm, Group 3; 3 x 1 mm, and Group 4-6; 3 x 2 mm respectively. Group 1-4 were restored using bulk filling method with Z-250 composite. However, Group 5 was restored incrementally, and Group 6 was restored with an indirect resin inlay. The cusp deflection was recorded at the buccal and lingual cusp tips using LVDT probe for 10,000 seconds. The measured cusp deflections were compared between groups, and the relationship between the cube of the length of cavity wall/the cube of the thickness of cavity wall (L3 / T3), C-factor and cusp deflection or %flexure (100 x cuspal deflection / cavity width) was analyzed. The cusp deflection of Group 1-4 were 12.1 microm, 17.2 microm, 16.2 microm and 26.4 microm respectively. The C-factor was related to the %flexure rather than the cusp deflection. There was a strong positive correlationship between the L3 / T3 and the cusp deflection. The cusp deflection of Group 5 and 6 were 17.4 microm and 17.9 microm respectively, which are much lower value than that of Group 4.
Bicuspid ; Inlays

OBJECTIVES: The aim of this study was to investigate the effect of monomer and filler compositions on the rheological properties related to the handling characteristics of resin composites. METHODS: Resin matrices that Bis-GMA as base monomer was blended with TEGDMA as diluent at various ratio were mixed with the Barium glass (0.7 um and 1.0 um), 0.04 um fumed silica and 0.5 um round silica. All used fillers were silane treated. In order to vary the viscosity of experimental composites, the type and content of incorporated fillers were changed. Using a rheometer, a steady shear test and a dynamic oscillatory shear test were used to evaluate the viscosity (eta) of resin matrix, and the storage shear modulus (G'), the loss shear modulus (G"), the loss tangent (tandelta) and the complex viscosity (eta*) of the composites as a function of frequency omega = 0.1-100 rad/s. To investigate the effect of temperature on the viscosity of composites, a temperature sweep test was also undertaken. RESULTS: Resin matrices were Newtonian fluid regardless of diluent concentration and all experimental composites exhibited pseudoplastic behavior with increasing shear rate. The viscosity of composites was exponentially increased with increasing filler volume%. In the same filler volume, the smaller the fillers were used, the higher the viscosities were. The effect of filler size on the viscosity was increased with increasing filler content. Increasing filler content reduced tandelta by increasing the G' further than the G". The viscosity of composites was decreased exponentially with increasing temperature.
Barium ; Bisphenol A-Glycidyl Methacrylate ; Glass ; Rheology ; Silicon Dioxide ; Viscosity

OBJECTIVE: The purpose of this study was to investigate the viscoelastic properties related to handling characteristics of composite resins. METHODS: A custom designed vertical oscillation rheometer (VOR) was used for rheological measurements of composites. The VOR consists of three parts: (1) a measuring unit, (2) a deformation induction unit and (3) a force detecting unit. Two medium viscous composites, Z100 and Z250 and two packable composites, P60 and SureFil were tested. The viscoelastic material function, including complex modulus E* and phase angle delta, were measured. A dynamic oscillatory test was used to evaluate the storage modulus (E'), loss modulus (E") and loss tangent (tandelta) of the composites as a function of frequency (omega) from 0.1 to 20 Hz at 23degrees C. RESULTS: The E' and E" increased with increasing frequency and showed differences in magnitude between brands. The E*s of composites at omega = 2 Hz, normalized to that of Z100, were 2.16 (Z250), 4.80 (P60) and 25.21 (SureFil). The magnitudes and patterns of the change of tandelta of composites with increasing frequency were significantly different between brands. The relationships between the complex modulus E*, the phase angle delta and the frequency omega were represented by frequency domain phasor form, E* (omega) = E*e(idelta) = E* 45 degree angle delta. CONCLUSIONS: The viscoelasticity of composites that influences handling characteristics is significant different between brands. The VOR is a relatively simple device for dynamic, mechanical analysis of high viscous dental composites. The locus of frequency domain phasor plots in a complex plane is a valuable method of representing the viscoelastic properties of composites.
Composite Resins

PURPOSE: Heat shock protein 27 (HSP27) is induced by heat shock and other pathophysiologic stresses. We examined the relationship between HSP27 expression and Gleason score and pathologic stage of prostate cancer. MATERIALS AND METHODS: Fifty-three men were treated by radical prostatectomy for prostate cancer diagnosed from May 2004 to April 2007. Prostate tissues (n=53) were obtained from radical prostatectomy specimens of prostate cancer. The overall percentage of cancer cells showing staining (0% to 100%) was indicated by visual scoring. Specimens were graded from +1 to +3 intensity representing the range of staining area, for which below 5% is +1 grade, 5-50% is +2 grade, over 50% is +3 grade and focal reaction is +0.5 grade. RESULTS: An HSP27-positive reaction was seen in 2 of 11 cases (18.2%) with a Gleason score of 4-6, 11 of 19 cases (57.9%) with a Gleason score 7, 6 of 10 cases (60.0%) with a Gleason score 8, 12 of 13 cases (92.3%) with a Gleason score of 9 (p=0.001); the mean HSP27 reaction scores were 0.27, 0.86, 0.83, and 1.54 respectively (p=0.006). An HSP27-positive reaction was seen in 17 of 37 cases (46.0%) with pathologic stage T2, 10 of 12 cases (83.3%) with pathologic stage T3, and 4 of 4 cases (100%) with pathologic stage T4 (p=0.0032); the mean HSP27 reaction scores were 0.64, 1.17, and 2, respectively (p=0.007). HSP27 expression was not statistically significant according to age. CONCLUSIONS: There is correlation between HSP27 expression and Gleason score, pathologic stage of prostate cancer.
Heat-Shock Proteins ; Hot Temperature ; HSP27 Heat-Shock Proteins ; Humans ; Male ; Neoplasm Grading ; Prostate ; Prostatectomy ; Prostatic Neoplasms ; Shock

Malignant mixed miillerian tumors(MMMTs) are uncommon neoplasms of the female genital tract that are histologically defined by the presence of malignant epithelial and stromal elements. MMMTs can arise hom the uterine body, cervix, vagina, ovary, and fallopian tube and constitute less than 1% of all overian malignancies. These tumors are usually advanced when diagnosed and follow an aggressive clinical course. We had experienced a cas#e of MMMT of the ovary and presented with a brief review of literature.
Cervix Uteri ; Fallopian Tubes ; Female ; Humans ; Ovary* ; Vagina

PURPOSE: The principle of prehospital transport is that the patient should be transported to the nearest hospital that is suitable for the severity of the illness. Therefore, out-of-jurisdiction transport is improper. The purpose of this study was to understand the present situation and evaluate the appropriateness of out-of-jurisdiction prehospital transport by the 119 rescue group. METHODS: Between January and December 2009 we enrolled patients who were transported to the Gwangju Regional Emergency Medical Center by the 119 rescue group, which belongs to Jeolla province. The appropriateness of out-of-jurisdiction transport was based on the transport chart and medical record of the 119 rescue group and categorized into three groups. RESULTS: The total number of patients transported was 440: 78 (17.7%) were appropriate; 329 (74.8%) were inappropriate; 33 (7.5%) patients were very inappropriate. Of the 440, 156 (35.5%) were emergency cases, 147 (33.4%) were sub-emergency cases, and 137 (31.1%) were non-emergency cases. Comparing these data with the triage by the 119 rescue group, the kappa value was 0.368 (p<0.001). The patients or their guardians selected the hospital to which the patient was be transported in 382 (86.8%) cases. The actual transport distance was 40.0 km (range: 26.0 to 50.0) and was significantly longer than the nearest distance to a local emergency center which was 10.0 km (4.0 to 18.0) (p<0.001). CONCLUSION: Inappropriate out-of-jurisdiction transporting of patients is done frequently. Therefore, guidelines for prehospital transportation according to the triage is necessary. Additionally, the medical director and emergency medicine information center could play a role in selecting a hospital. A fee or a fine of transporters who do not observe the guidelines might limit the proportion of inappropriate transport cases.
Ambulances ; Emergencies ; Emergency Medical Services ; Emergency Medicine ; Fees and Charges ; Humans ; Information Centers ; Medical Records ; Physician Executives ; Transportation ; Transportation of Patients ; Triage

PURPOSE: Because low-quality trials may lead to erroneous conclusions, quality assessments are necessary. Thus, in this study, we scrutinized randomized controlled trials (RCTs) published in the Korean Journal of Urology (KJU) to assess their quantity and quality. MATERIALS AND METHODS: Upon extracting RCTs from all articles published in the KJU from 1991 to 2010, assessments were made on the basis of the Jadad scale and the adequacy of allocation concealment. The selections and assessments were performed independently by two researchers, and adjustment of the differences was done by a third-party researcher. In addition, the factors that may affect quality were analyzed. RESULTS: A total of 3,516 original articles were searched and 28 RCTs were extracted. In the 1990s, RCTs constituted only 0.27% of the total original articles, but in the 2000s, RCTs constituted 1.34%. The mean total Jadad score increased from 1.6 points in the 1990s to 1.65 points in the 2000s. However, the percentage of "good quality" trials also increased from 20% to 30.43%. As for adequate allocation concealment, one study was observed in the 2000s. The aspect most lacking was appropriate dropout and double-blinding. Studies with medical interventions or funded or examined by institutional review boards tended to receive higher quality assessments. CONCLUSIONS: Although RCTs consistently increased in both quantity and quality, in future studies, researchers should continue to strive toward achieving adequate allocation concealment and appropriate double-blinding. In addition, researchers must become more interested in receiving external funding and undergoing examination by institutional review boards.
Ethics Committees, Research ; Financial Management ; Humans ; Korea ; Patient Dropouts ; Prospective Studies ; Random Allocation ; Urology

PURPOSE: We performed an analysis of the smartphone legibility of the websites of the Korean Urological Association (KUA) and other urological societies. MATERIALS AND METHODS: This study was conducted on the websites of the KUA and nine other urological societies. Each website was accessed via iPhone Safari and Android Chrome, respectively, to evaluate the establishment and readability of the mobile web pages. The provision of Really Simple Syndication (RSS) feeds by the websites and whether the websites had Twitter and Facebook accounts were evaluated. In addition, a validation test on the web standards was performed by using the World Wide Web Consortium (W3C(R)) Markup Validation Service, and subsequently the numbers of errors and warnings that occurred were analyzed. RESULTS: When accessed via Safari, two websites were legible, four were somewhat legible, and four were somewhat illegible. When accessed via Chrome, two websites were legible, six were somewhat legible, and two were somewhat illegible. One website provided an RSS feed and two websites managed members via separate Twitter accounts. No website supported mobile web pages. The result of the W3C(R) Markup Validation test on 10 websites showed a mean error rate of 221.6 (range, 13-1,477) and a mean warning rate of 127.13 (range, 0-655). CONCLUSIONS: The smartphone legibility level of the websites of urological societies was relatively low. Improved smartphone legibility and web standard compliance of the websites of urological societies are required to keep up with the popularity of smartphones.
Compliance ; Comprehension ; Internet ; Korea ; Urology