2.Three case of leptomenigeal metastasis from solid tumors: diagnosedby cytocentrifugation and LDH isoenzyme.
Jin Woo YOO ; Yiel Hea SEO ; Sang Gyung KIM ; Wha Soon CHUNG ; Woong Soo LEE
Korean Journal of Clinical Pathology 1991;11(3):583-587
No abstract available.
Neoplasm Metastasis*
3.Metabolism of C(14)-acetate by some trematodes.
Byong Seol SEO ; Han Jong RIM ; Yong Ok MIN ; Sang Don RHEE ; Tong Hoon LEE ; Myong Soon YUN
The Korean Journal of Parasitology 1965;3(3):117-121
The adult trematodes, Fasciola hepatica, Eurytrema pancreaticum and Paramphistomum cervi, employed in this experiment were obtained from the cattle slaughtered at the local abbatoir. The worms selected and washed several times in normal sterilized saline solution. Each about ten of intact F. hepatica, fourty of E. pancreaticum, and twenty of P. cervi were incubated in 50 cc volume of special incubation flasks with incubation medium consisting of 10 cc. of Krebs-Ringer phosphate buffer(pH 7.4) The incubation medium was added C(14)-1-acetate and non-radioactive carrier Na-acetate so as to contain acetate concentration of 50 mg per cent . The worms were allowed to incubate for 5 hours in the Dubnoff metabolic shaking incubator at 38 C. After incubation period, respiratory CO(2) samples from central well of incubation flask were analysed for total CO(2) production rate and their specific activity of respiratory CO(2). The lactate and pyruvate appearance rates were determined by analyzing the lactate and pyruvate concentration in a medium after incubation. The glycogen samples isolated from worms were analyzed for the tissue concentration and their radioactivities in order to determine the turnover rate of glycogen pool. Radioactivities of these series of experiments were counted by an endwindow Geiger-Muller counter as an infinitely thin samples. The quantitative analysis of C(14)-acetate utilized by F. hepatica, E. pancreaticum and P. cervi were compared and discussed in this report. According to these data of the experiment, it is suggested that the fatty acid such as acetate may play a part of their oxidative process into the respiratory CO2 and the synthetic process into glycogen in the above species of trematodes.
parasitology
;
helminth
;
trematoda
;
Fasciola hepatica
;
Eurytrema pancreaticum
;
Paramphistomum cervi
;
acetate
;
metabolism
;
biochemistry
;
CO(2)
;
glycogen
;
Krebs-Ringer phosphate buffer
4.The Protective Effect of Calcium Antagonist on Myocardium in Coronary Reperfusion Following Experimental Myocardial Infarction.
June Key CHUNG ; Sang Moo LIM ; Myung Chul LEE ; Chang Soon KOH ; Munho LEE ; Jeong Wook SEO ; Norman D LAFRANCE
Korean Circulation Journal 1987;17(4):719-733
Although it has been suggested that the calcium antagonist verapamil has beneficial effects on ischemic myocardium, its effect during coronary reperfusion has not been studied in detail. The purpose of this study was to investigate the inhibitory effect of verapamil on myocardial damage quantitatively using 111 In-anticardiac myosin antibody (ACM Ab) and qualitatively using electronmicroscopic method. Anesthetized open-chest dogs were subjected to 1 hour of occlusion of the left anterior descending coronary artery (LAD) followed by 90 minutes of reperfusion. Regional myocardial blood flow was determined by injecting 85Sr-microsphere prior to LAD reperfusion, and regional myocardial damage was measured by injecting 111In-ACm Ab at 30 minutes after LAD reperfusion. Six dogs were randomly selected as saline control and verapamil-treated (0.6 mg/kg. hr) groups each. Saline or verapamil was infused at 40 minutes after LAD occlusion and continued through the experiment. 1) Verapamil produced significant (P<0.05 by Wilcoxon rank sum test) decrease in heart rate, mean arterial blood pressure and double product. There was no significant change in pulmonary hemodynamics or cardiac output. 2) Stroke volume was reduced significantly (P<0.05 by Wilcoxon rank sum test) after 30 minutes of LAD reperfusion in the control group, but it was preserved in the verapamil-treated group. 3) There was an inverse exponential relationship between 111In-ACm Ab localization and regional blood flow in both control (r=-0.86) and verapamil treated (r=-0.71) groups. Significant difference between the two groups was found in exponential curve (p[t]<0.05). 4) A lesser uptake of 111in-ACM Ab was observed in the verapamil treated group compared with that in the control group in the region where the regional blood flow was lower than 30+/- of normal. 5) In the control group, the myocardium showed swelling, contraction bands, and electron dense granules in the mitochondria which were proven to be calcium aggregates. In the verapamiltreated grooup, the myocardium showed fewer electro dense granules and mild degree of contraction bands. This study supports the concept that verapamil reduces the myocardial damage following coronary reperfusion in myocardial infarction and may reduce contraction band necrosis.
Animals
;
Arterial Pressure
;
Calcium*
;
Cardiac Output
;
Coronary Vessels
;
Dogs
;
Heart Rate
;
Hemodynamics
;
Mitochondria
;
Myocardial Infarction*
;
Myocardial Reperfusion*
;
Myocardium*
;
Myosins
;
Necrosis
;
Regional Blood Flow
;
Reperfusion
;
Stroke Volume
;
Verapamil
5.A Case of Hyperparathyroidism induced from Cystic Parathyroid Adenoma.
Ki Chul SUNG ; Kwon CHOI ; Won Tae SEO ; Soon Ho KWON ; Sang Jong LEE ; Joo Seob KEUM ; Myung Sook KIM
Journal of Korean Society of Endocrinology 1997;12(1):75-80
Cystic parathyroid adenoma is one of the rare causes of hyperparathyroidism, and is usually located in neck and mediastinum. This type of adenoma tends to cause increased serum level of parathyroid hormone and alkaline phosphatase, similar serum calcium level when compared to those of solid adenoma. Thanks to current radioimmunoassay and easy accessibility to serum autoanalyzer, serum calcium level and parathyroid hormone level are more easily measured, which in turn lead to more easier diagnosis of hyperparathyroidism. Also improvement in imaging and nuclear diagnostic method of parathyroid lesion are suggested to enable easy diagnosis of cystic parathyroid adenoma. A 35-year-old male presented with easy fatigability for 12 months. The serum calcium, phosphate, alkaline phosphotase were 11.5mg/dL, 1.4mg/dL, 194IU/L respectively and his parathyroid hormone level in serum was 126.42pg/mL. Neck CT showed enlargement of right lobe of thyroid gland with well defined inhomogenously enhanced density inside the right thyroid gland. The patient was diagnosed of hyperparathyroidim due to parathyroid adenoma and was surgically removed. The surgical biopsy showed cystic parathyroid adenoma. After operation his general condition was improved and serum calcium, phosphate, parathyroid hormone level were normalizd. We report a case of hyperparathyroidism caused by cystic parathyroid adenoma with brief review of literature.
Adenoma
;
Adult
;
Alkaline Phosphatase
;
Biopsy
;
Calcium
;
Diagnosis
;
Humans
;
Hyperparathyroidism*
;
Male
;
Mediastinum
;
Neck
;
Parathyroid Hormone
;
Parathyroid Neoplasms*
;
Radioimmunoassay
;
Thyroid Gland
6.Procoagulant activity observed in human umbilical vein endothelial cell line cells infected with Hantaan virus.
Sang In CHUNG ; Jeong Gook SEO ; Eung Taek KANG ; Suk Hee YU ; Chul Soon CHOI ; Yong Tae YANG
Journal of the Korean Society of Virology 1992;22(2):169-179
No abstract available.
Endothelial Cells*
;
Hantaan virus*
;
Humans*
;
Umbilical Veins*
7.Localization of cytoskeletal proteins in Pneumocystis carinii by immuno-electron microscopy.
Jae Ran YU ; Jae Kyong PYON ; Min SEO ; Byung Suk JUNG ; Sang Rock CHO ; Soon Hyung LEE ; Sung Tae HONG
The Korean Journal of Parasitology 2001;39(1):13-21
Pneumocystis carinii causes serious pulmonary infection in immunosuppressed patients. This study was undertaken to observe the cytoskeletal proteins of P. carinii by immuno-electron microscopy. P. carinii infection was experimentally induced by immunosuppression of Sprague-Dawley rats for seven weeks, and their lungs were used for the observations of this study. The gold particles localized actin, tropomyosin, and tubulin. The actin was irregularly scattered in the cytoplasm of the trophic forms but was much more concentrated in the inner space of the cell wall of the cystic forms called the inner electron-lucent layer. No significant amount of tropomyosin was observed in either trophic forms or cystic forms. The tubulin was distributed along the peripheral cytoplasm and filopodia of both the trophic and cystic forms rather than in the inner side of the cytoplasm. Particularly, in the cystic forms, the amount of tubulin was increased and located mainly in the inner electron-lucent layer of the cell wall where the actin was concentrated as well. The results of this study showed that the cell wall of P. carinii cystic forms is a structure whose inner side is rich in actin and tubulin. The location of the actin and tubulin in P. carinii suggests that the main role of these proteins is an involvement in the protection of cystic forms from the outside environment by maintaining rigidity of the cystic forms.
Actins/analysis
;
Animals
;
Cytoskeletal Proteins/*analysis
;
Fungal Proteins/*analysis
;
Histocytochemistry
;
Microscopy, Immunoelectron
;
Pneumocystis/*chemistry/cytology
;
Rats
;
Rats, Wistar
;
Support, Non-U.S. Gov't
;
Tropomyosin/analysis
;
Tubulin/analysis
8.A case of Sertoli-Leydig cell tumor.
Sang Duk SHIM ; Wan Young KIM ; Dae Sik SEO ; Young Min CHOI ; Soon Bum KANG ; Hyo Pyo LEE
Korean Journal of Obstetrics and Gynecology 1992;35(7):1096-1103
No abstract available.
Sertoli-Leydig Cell Tumor*
9.Clinical Efficacy of Ifosfamide-Based Regimen in Refractory of Relapsed Ovarian Cancer.
Hyo Pyo LEE ; Noh Hyun PARK ; Jae Weon KIM ; Seo Young PARK ; Yong Sang SONG ; Soon Beom KANG
Korean Journal of Gynecologic Oncology and Colposcopy 1996;7(4):263-269
This phase II study aimed to assess the clinical activity and toxicity of ifosfamide based regimen in patients with epithelial ovarian cancer, relapsed or refractory to cisplatin-containing combination chemotherapy. From July 1991 to June 1993, 18 patients with epithelial ovarian cancer, relapsed or refractory to cisplatin were treated as follows. Relapsed cases were treated with IP(ifosfamide 4,0g/m2 intravenously and cisplatin 60mg/m2 intravenously on day 1) regimen every 3-4 weeks. The regimen used in refractory cases was Et-I(etoposide 100mg/m2 intravenously on days 1 to 3 and ifosfamide 1.0g/m2 intravenously on days 1 to 5) regimen every 3 or 4 weeks. The uroprotectant mesna was concomitantly used. Responses and toxicities were evaluated according to the WHO Criteria. The overall response rate was 27.8%(5/18), including 2 complete response and 3 partial response. There were four episodes(22.2%) of grade 3, 4 myelosuppression, but no other grade 3, 4 non-hematologic toxicity. Salvage therapy with ifosfamide based regimen is a useful and well tolerated treatment strategy in selected patients with relapsed ovarian cancer.
Cisplatin
;
Drug Therapy, Combination
;
Humans
;
Ifosfamide
;
Mesna
;
Ovarian Neoplasms*
;
Recurrence
;
Salvage Therapy
10.Management and Treatment of Drooling: Focus on Non-Invasive Therapy for Children
Kyoung-chul MIN ; Sang-min SEO ; Hee-soon WOO
Journal of the Korean Dysphagia Society 2021;11(2):111-120
Drooling is defined as the unintentional flow of saliva out of the mouth or to the back of the pharynx due to salivary control problems and swallowing difficulties. Drooling is caused by posture, oral sensori-motor and cognitive problems, and medical symptoms. Treatments of drooling are defined under two criteria: 1) non-invasive treatment, which includes posture and oral motor therapy, and 2) invasive treatment, including botox injection and surgery.Drooling treatment should be initiated non-invasively and in a step-wise manner in all individuals. This mode of treatment is safe, age-free, and is considered the first line of therapy, before subjecting to invasive treatment.Clinical professionals provide non-invasive treatment via manual approaches, through passive sensori-motor stimulation to active oral motor activities. Comprehensive therapy protocols such as oral motor facilitation technique (OMFT) for oral sensory normalization, head and neck postural control, and oral motor function improvements, are required to help control drooling. Clinical professionals therefore need to deeply understand the reason for drooling, and provide qualified non-invasive treatment for intensifying drooling control.