We encountered the danger of the bleeding the vertex and posterior fossa epidural hematoma(EDH) operation which were related with large sinuses. Traumatic vertex EDH and posterior fossa EDH were usually accompanied by the fractures of coronal, sagittal suture or occipital bone. And frequently, sources of the bleeding were venous sinuses or fracture site. The patients who had vertex EDH were paraparetic soon after injury and the patients who had posterior fossa EDH were dangerous due to brain stem compression. We treated successfully the 13 patients who had vertex EDH(7 patients) and posterior fossa EDH(6 patients) by the method of one or two burr holes and urokinase irrigation. The authors represented this method may occasionally be preferable to craniotomy for these lesions. Advantages of the methods were : 1) More simple, cosmatic procedure and no need of cranioplasty. 2) Reduced operation time and morbidity. 3) Enable to operate under local anesthesia. 4) Reduced rate of delayed intracranial hematoma due to acute decompression of epidural hematoma. 5) Reduced the date of admission. 6) Possibly, prevention of rebleeding and air embolism.
Anesthesia, Local ; Brain Stem ; Craniotomy ; Decompression ; Embolism, Air ; Hematoma* ; Hemorrhage ; Humans ; Occipital Bone ; Sutures ; Urokinase-Type Plasminogen Activator*

No abstract available.
Blood Donors* ; HIV* ; Humans ; Mass Screening*

No abstract available.
Analgesia*

Epidermoid cyst is a congenital tumor and it's incidence is relatively rare in all intracranial tumors. Preference site is the basal surface of the brain, particularly the cerebellopontine angle and parasellar region. We report a epidermoid cyst in the fourth ventricle of 37 years old femals with signs of cerebellar dysfunctions. The literature on the incidence, diagnosis, surgical technoque and complications are reviewed.
Adult ; Brain ; Cerebellar Diseases ; Cerebellopontine Angle ; Diagnosis ; Epidermal Cyst* ; Fourth Ventricle* ; Humans ; Incidence

Epidural morphine has been used successfully for the relief of acute or chronic pain by direct injection or catheter method. We report the use of morphine for postoperative pain control in 18 herniated lumbar disc patients. These patients were compaired with 25 others who were not received epidural morphine. Without using of epidural catheter, single small dose morphine(3~5mg) in 1.5ml normal saline was administered under the direct infution into the epidural space just before closure of the lumbar operative wound. Epidural morphine group required only 2 times of parenteral narcotics injections within the first 24 hours after lumbar laminectomy, whereas nonmorphine group required 48 injections. The average narcotics injection frequencies were statistically significant(p<0.01). Hypotension were occured in three cases and pruritus in two cases. But more investication and study were needed for hypotension which was true side effect or not. Respiratory depression was not occured.
Catheters ; Chronic Pain ; Epidural Space ; Humans ; Hypotension ; Laminectomy* ; Morphine* ; Narcotics ; Pain, Postoperative ; Pruritus ; Respiratory Insufficiency ; Wounds and Injuries

We report a case of 54-year-old female with two intracranial tumors, a meningioma and multifocal glioblastoma multiforme with extracranial metastasis. The simultaneous occurrence of meningioma and glioblastoma multiforme and extracranial metastasis of glioblastoma multiforme are rare. We demonstrated additional clinical and pathological features of interest and reviewed the relevant literatures, and have speculated on the causes of this phenomenon.
Female ; Glioblastoma* ; Humans ; Meningioma* ; Middle Aged ; Neoplasm Metastasis

The nfa1 gene was cloned from a cDNA library of pathogenic Naegleria fowleri by immunoscreening; it consisted of 360 bp and produced a 13.1 kDa recombinant protein (rNfa1) that showed the pseudopodia-specific localization by immunocytochemistry in the previous study. Based on the idea that the pseudopodia-specific Nfa1 protein mentioned above seems to be involved in the pathogenicity of N. fowleri, we observed the effect of an anti-Nfa1 antibody on the proliferation of N. fowleri trophozoites and the cytotoxicity of N. fowleri trophozoites on the target cells. The proliferation of N. fowleri trophozoites was inhibited after being treated with an anti-Nfa1 polyclonal antibody in a dose-dependent manner for 48 hrs. By a light microscope, CHO cells co-cultured with N. fowleri trophozoites (group I) for 48 hrs showed severe morphological destruction. On the contrary, CHO cells co-cultured with N. fowleri trophozoites and anti-Nfa1 polyclonal antibody (1: 100 dilution) (group II) showed less destruction. In the LDH release assay results, group I showed 50.6% cytotoxicity, and group II showed 39.3%. Consequently, addition of an anti-Nfa1 polyclonal antibody produced a decreasing effect of in vitro cytotoxicity of N. fowleri in a dosedependent manner.
Animals ; Antibodies, Protozoan/*immunology ; Antigens, Protozoan/genetics/*immunology ; CHO Cells ; Dose-Response Relationship, Immunologic ; Female ; Hamsters ; Mice ; Mice, Inbred BALB C ; Naegleria fowleri/growth & development/immunology/*pathogenicity ; Protozoan Proteins/genetics/*immunology ; Recombinant Proteins/immunology ; Support, Non-U.S. Gov't

Thrombolysis using urokinase solution is one of the effective methods in treatment of intracerebral hematoma. The present study was undertaken in order to 1) determine the most effective concentration of urokinase solution, 2) determine the most suitable time interval of irrigation of urokinase solution through the measurement of hemoglobin and FDP(Fibrin / Fibrinogen Degradation Product) of drained solution, 3) estimate the size of unresolved hematoma without taking brain CT. The results are summarized as follows : 1) The most effective and economic concentration of urokinase solution was 1000 u/ml. 2) The most preferable time interval of irrigation of urokinase solution was about one hour. 3) It was possible to estimate the size of unresolving hematoma by means of measurement of hemoglobin and hematocrit of patient, hemoglobin and volume of the thrombolysed solution, so that it was unnecessary to take brain CT for measurement of remaining hematoma.
Brain ; Fibrinogen ; Hematocrit ; Hematoma* ; Humans ; Urokinase-Type Plasminogen Activator*

Sec13p has been known as an endoplasmic reticulum-Golgi transport protein. Recently, it has also been shown to be required for the formation of septation in the fission yeast Schizosaccharomyces pombe. In the present study, we focused on the role of a human homolog of Saccharomyces cerevisiae SEC13, Sec13 protein during mitosis in U2OS cells. We found that the expression of Sec13 was constant throughout the cell cycle, and localized to the kinetochores at metaphase during mitosis. By using green fluorescent protein technology, we observed that Sec13 is required for evasion of mitotic arrest in response to spindle damage, leading to G1-like phase and apoptotic cell death. In addition, cells expressing exogenous Sec13 showed giant nuclei compared to endogenous ones in the absence of nocodazole. These results demonstrate that Sec13 is involved in the regulation of the metaphase/anaphase transition and may be functionally associated with mitotic machinery to maintain genomic stability during mitosis.
Anaphase ; Antineoplastic Agents/pharmacology ; Cell Line, Tumor/drug effects/metabolism/pathology ; *G1 Phase ; *Genomic Instability ; Green Fluorescent Proteins/metabolism ; Humans ; Kinetochores/metabolism ; Membrane Proteins/*genetics/metabolism ; Metaphase ; Mitosis/*physiology ; *Mitotic Spindle Apparatus ; Nocodazole/pharmacology ; Osteosarcoma/genetics/metabolism/pathology ; Research Support, Non-U.S. Gov't

Congenital epulis of the newborn refers to a very rare gingival tumor that occurs along the anterior alveolar ridge in newborn infants. We report a neonate with 3*2*1.5cm mass protruding from the mouth. This was a pinky, red solid pedunculated mass, attached to the maxillar left canine region of the alveolus at birth. The tumor's large size partially obliterated the oral cavity and caused oral feeding difficulty but did not cause respiratory problems. We described the postnatal MR imaging findings, electromicroscopic, histochemical and immunohistochemical studies of this tumor in order to search for the histogenesis of the tumor. In our case, histochemically, individual tumor cells contained numerous PAS positive cytoplasmic granules. Immunohistochemically, strong and diffuse cytoplasmic staining for vimentin and NSE was observed. Staining with S-100 protein, cytokeratin, desmin, CEA, factor VIII-related antigen, lysozyme, EMA were negative. After total excision of the lesion in our case, we had no complication or recurrence either early or late(up to one year).
Alveolar Process ; Cytoplasm ; Cytoplasmic Granules ; Desmin ; Gingival Neoplasms* ; Humans ; Infant, Newborn* ; Keratins ; Magnetic Resonance Imaging ; Mouth ; Muramidase ; Parturition ; Recurrence ; S100 Proteins ; Vimentin ; von Willebrand Factor