1.A case of Prune Belly syndrome associated with Turner syndrome.
Chang Soo OH ; Sang Muk CHOI ; Jae Ock PARK ; Chang Hwi KIM ; Sang Jhoo LEE
Journal of the Korean Pediatric Society 1992;35(7):978-983
No abstract available.
Prune Belly Syndrome*
;
Turner Syndrome*
2.Ultrasonographic measurement of subclavian vein diameter and regression modeling in pediatric patients from a single Korean facility.
Min Hye OH ; Woo Suk CHUNG ; Yo Han KIM ; Byung Muk KIM ; Sang Il PARK
Korean Journal of Anesthesiology 2014;67(Suppl):S96-S97
No abstract available.
Humans
;
Subclavian Vein*
3.SUMO Proteins are not Involved in TGF-beta1-induced, Smad3/4-mediated Germline alpha Transcription, but PIASy Suppresses it in CH12F3-2A B Cells.
Sang Hoon LEE ; Pyeung Hyeun KIM ; Sang Muk OH ; Jung Hwan PARK ; Yung Choon YOO ; Junglim LEE ; Seok Rae PARK
Immune Network 2014;14(6):321-327
TGF-beta induces IgA class switching by B cells. We previously reported that Smad3 and Smad4, pivotal TGF-beta signal-transducing transcription factors, mediate germline (GL) alpha transcription induced by TGF-beta1, resulting in IgA switching by mouse B cells. Post-translational sumoylation of Smad3 and Smad4 regulates TGF-beta-induced transcriptional activation in certain cell types. In the present study, we investigated the effect of sumoylation on TGF-beta1-induced, Smad3/4-mediated GLalpha transcription and IgA switching by mouse B cell line, CH12F3-2A. Overexpression of small ubiquitin-like modifier (SUMO)-1, SUMO-2 or SUMO-3 did not affect TGF-beta1-induced, Smad3/4-mediated GLalpha promoter activity, expression of endogenous GLalpha transcripts, surface IgA expression, and IgA production. Next, we tested the effect of the E3 ligase PIASy on TGF-beta1-induced, Smad3/4-mediated GLalpha promoter activity. We found that PIASy overexpression suppresses the GLalpha promoter activity in cooperation with histone deacetylase 1. Taken together, these results suggest that SUMO itself does not affect regulation of GLalpha transcription and IgA switching induced by TGF-beta1/Smad3/4, while PIASy acts as a repressor.
Animals
;
B-Lymphocytes*
;
Cell Line
;
Histone Deacetylase 1
;
Immunoglobulin A
;
Immunoglobulin Class Switching
;
Mice
;
Small Ubiquitin-Related Modifier Proteins*
;
SUMO-1 Protein*
;
Sumoylation
;
Transcription Factors
;
Transcriptional Activation
;
Transforming Growth Factor beta
;
Transforming Growth Factor beta1
;
Ubiquitin-Protein Ligases
4.Withaferin A Inhibits Helicobacter pylori-induced Production of IL-1beta in Dendritic Cells by Regulating NF-kappaB and NLRP3 Inflammasome Activation.
Jae Eun KIM ; Jun Young LEE ; Min Jung KANG ; Yu Jin JEONG ; Jin A CHOI ; Sang Muk OH ; Kyung Bok LEE ; Jong Hwan PARK
Immune Network 2015;15(6):269-277
Helicobacter pylori infection is associated with chronic gastritis, peptic ulcer, and gastric cancer. There is evidence that IL-1beta is associated with the development of gastric cancer. Therefore, downregulation of H. pylori-mediated IL-1beta production may be a way to prevent gastric cancer. Withaferin A (WA), a withanolide purified from Withania somnifera, is known to exert anti-inflammatory and anti-tumor effects. In the present study, we explored the inhibitory activity of WA on H. pylori-induced production of IL-1beta in murine bone marrow-derived dendritic cells (BMDCs) and the underlying cellular mechanism. Co-treatment with WA decreased IL-1beta production by H. pylori in BMDCs in a dose-dependent manner. H. pylori-induced gene expression of IL-1beta and NLRP3 (NOD-like receptor family, pyrin domain containing 3) were also suppressed by WA treatment. Moreover, IkappaB-alpha phosphorylation by H. pylori infection was suppressed by WA in BMDCs. Western blot analysis revealed that H. pylori induced cleavage of caspase-1 and IL-1beta, as well as increased procaspase-1 and pro IL-1beta protein levels, and that both were suppressed by co-treatment with WA. Finally, we determined whether WA can directly inhibit ac tivation of the NLRP3 inflammasome. NLRP3 activators induced IL-1beta secretion in LPS-primed macrophages, which was inhibited by WA in a dose-dependent manner, whereas IL-6 production was not affected by WA. Moreover, cleavage of IL-1beta and caspase-1 by NLRP3 activators was also dose-dependently inhibited by WA. These findings suggest that WA can inhibit IL-1beta production by H. pylori in dendritic cells and can be used as a new preventive and therapeutic agent for gastric cancer.
Blotting, Western
;
Caspase 1
;
Dendritic Cells*
;
Down-Regulation
;
Gastritis
;
Gene Expression
;
Helicobacter pylori
;
Helicobacter*
;
Humans
;
Interleukin-1beta
;
Interleukin-6
;
Macrophages
;
NF-kappa B*
;
Peptic Ulcer
;
Phosphorylation
;
Stomach Neoplasms
;
Withania
5.Synchronous double cancer of rectal non-Hodgkin lymphoma and lung cancer: 1 case report.
Hyeon Jeong KIM ; Kwang Soon ANN ; Ji Hyeon OH ; Moo Yeol LEE ; Hoon CHO ; Young Muk KIM ; Joon Sang LEE
Korean Journal of Medicine 2006;70(4):462-466
Colorectal lymphomas constitute 10~20% of gastrointestinal lymphomas and arerelatively rarer than lymphomas which involve stomach or small intestine. Rectal lymphomas compose fewer than 1% of malignant rectal tumors. Although there are several case reports and reviews about non-Hodgkin lymphoma associated with primary lung cancer in Korea, the case of synchronous double cancer of rectal lymphoma and primary lung cancer was not reported yet. We report a case of synchronous double primary cancer of rectal lymphoma and squamous cell carcinoma of lung in 71-year-old man who presented with incidental right upper lung mass.
Aged
;
Carcinoma, Squamous Cell
;
Humans
;
Intestine, Small
;
Korea
;
Lung Neoplasms*
;
Lung*
;
Lymphoma
;
Lymphoma, Non-Hodgkin*
;
Rectal Neoplasms
;
Stomach
6.Fra - 1 expression in malignant and benign thyroid neoplasm.
Yong Hyun KIM ; Jeong Heon OH ; Nan Hee KIM ; Kyung Muk CHOI ; Sang Jin KIM ; Sei Hyun BAIK ; Dong Seop CHOI ; Eung Seok LEE
Korean Journal of Medicine 2000;59(4):398-403
BACKGROUND: Differential diagnosis of thyroid nodule is important in deciding treatment modality and fine needle aspiration is a good method to do so. But, sometimes, it has limitation in use because of inadequate test material and difficulty in interpreting it. Among the study of oncogene and tumor suppresor gene on the origin of thyroid tumor, expression of Fra-1, one of AP-1 system, is increased in thyroid neoplasm. So there is a possibility that it would be used as a method for differential diagnosis of thyroid nodule. We tried to know whether presence or absence of Fra-1 expression can be used as a diagnostic method in differential diagnosis of thyroid nodule using immuno- histochemical(IHC) staining method. METHODS: In 4 types of thyroid tumor that was confirmed by histologic diagnosis after operation(30 cases of papillary cancer, 10 cases of follicular cancer, 16 cases of follicular adenoma, 18 cases of adenomatous goiter), IHC staining method was performed to evaluate the expression of Fra-1. RESULTS: In papillary and follicular thyroid cancer, the expression of Fra-1 was stronger than benign thyroid tumor, but there was no difference in Fra-1 expression between two types of carcinoma. Weak expression of Fra-1 was observed in all cases of follicular adenoma, and it was also weakly expressed in 6 out 18 cases of adenomatous goiter. CONCLUSION: The expression of Fra-1 was stronger in thyroid cancer than in benign thyroid adenoma, but it was impossible to differentiate thyroid cancer from benign thyroid adenoma by the presence or absence of Fra-1 expression using IHC staining method.
Adenoma
;
Biopsy, Fine-Needle
;
Diagnosis
;
Diagnosis, Differential
;
Goiter
;
Immunohistochemistry
;
Oncogenes
;
Thyroid Gland*
;
Thyroid Neoplasms*
;
Thyroid Nodule
;
Transcription Factor AP-1
7.The effect of propofol for conscious sedation during colonoscopy-A Prospective, Randomized Study.
Hoon CHO ; Yeong Muk KIM ; Ji Hyeon OH ; Su Jin JEON ; Jae Hyeon MOON ; Min Hee KIM ; Keum Mi PARK ; Joon Sang LEE
Korean Journal of Medicine 2005;69(1):30-38
BACKGROUND: The objectives of this study were to assess propofol as sedative agents for colonoscopy in the safety and efficacy, compared with midazolam plus meperidine. METHODS: 120 patients undergoing colonoscopy in a prospective study were randomly assigned to one of three medication regimens. Patients in group A (n=42) received i.v. 25 mg of meperidine and 2.5 mg of midazolam. Patients in group B (n=41) received i.v. a median dose of 96.59 mg of propofol (range 40-180) and patients in group C (n=37) received i.v. 25 mg of meperidine and a median dose of 77.03 mg of propofol (range 40-150), administered by a nurse with endoscopist supervision. Endpoints were patient satisfaction, procedure and recovery times and complications. RESULTS: The mean time to sedation was much faster in 2 groups using propofol (group A: 152+/-105.9 sec, group B: 52.9+/-46.5 sec, group C: 56+/-63.7sec; p<0.001). The mean time to reach the cecum was not different among the 3 groups. There were total 3 episodes of oxygen desaturation to <90%, as one in each group, but the patients were spontaneously recovered with only increasing O2 concentration and arousal. Patients in group C expressed better overall mean satisfaction than patients in group A (p=0.049), but difference was not found between group A and group B. The amnestic effect was better in two groups using propofol than group A (p=0.017) The mean dose of propofol was lower in the patients who received propofol combined with low dose of meperidine than those with propofol only (77.03 mg vs 96.59 mg, p=0.009). CONCLUSION: Nurse-administered propofol sedation with endoscopist supervision is believed to be safe and useful for colonoscopy with careful monitoring.
Arousal
;
Cecum
;
Colonoscopy
;
Conscious Sedation*
;
Humans
;
Meperidine
;
Midazolam
;
Organization and Administration
;
Oxygen
;
Patient Satisfaction
;
Propofol*
;
Prospective Studies*
8.Fra-1 Expression in Malignant and Benign Thyroid Tumor.
Yong Hyun KIM ; Jeong Heon OH ; Nan Hee KIM ; Kyung Muk CHOI ; Sang Jin KIM ; Sei Hyun BAIK ; Dong Seop CHOI ; Eung Seok LEE
The Korean Journal of Internal Medicine 2001;16(2):93-97
BACKGROUND: The differential diagnosis of thyroid nodules is very important in deciding the treatment modality and the fine needle aspiration is the best diagnostic method. But, there are some limitations in use because of inadequate test materials and difficulty in interpreting. According to the study of oncogene and tumor suppressor gene about the origin of thyroid tumor, expression of Fra-1, one of AP-1 complex, is increased in thyroid neoplasm, though not present in the normal tissue. So, there is a possibility that it will be used as a method for the differential diagnosis of thyroid nodules. We tried to know whether presence or absence of Fra-1 expression can be used as a diagnostic method in differential diagnosis of thyroid nodules using the immunohistochemical (IHC) staining method. METHOD: In 4 types of thyroid tumor that were confirmed by histologic diagnosis after operation (18 cases of adenomatous goiter, 16 cases of follicular adenoma, 30 cases of papillary cancer, 10 cases of follicular cancer), IHC staining method was performed to evaluate the expression of Fra-1. RESULT: In papillary and follicular thyroid cancers, the expression of Fra-1 was stronger than in benign thyroid tumor, but there was no difference in Fra-1 expression between the two types of carcinoma. Weak expression of Fra-1 was observed in all cases of follicular adenoma, though it was weaker than in carcinoma, and it was also weakly expressed only in some cases (33%) of adenomatous goiter. CONCLUSION: The expression of Fra-1 was stronger in thyroid cancer than in benign thyroid tumor, but it was impossible to differentiate thyroid cancer from benign thyroid tumor by presence or absence of Fra-1 expression using IHC staining method.
Biopsy, Needle
;
Comparative Study
;
Diagnosis, Differential
;
Human
;
Immunohistochemistry
;
Proto-Oncogene Proteins c-fos/*analysis
;
Sensitivity and Specificity
;
Thyroid Diseases/metabolism/pathology
;
Thyroid Neoplasms/*metabolism/*pathology
;
Tissue Culture
;
Tumor Markers, Biological/*analysis
9.Patient-Controlled Sedation versus Nurse-Administered Sedation with Propofol during Colonoscopy.
Jee Hyun OH ; Hoon CHO ; Yeung Muk KIM ; Mu Yeul LEE ; Guang Soon AN ; Hyun Jeung KIM ; Hyun Gwang JUNG ; Kang Min KIM ; Joon Sang LEE
Korean Journal of Gastrointestinal Endoscopy 2005;31(1):32-38
BACKGROUND/AIMS: Patient-controlled sedation (PCS) allows the patients to titrate the dosages of sedative drug according to their needs. The objective of this study was to compare the safety and the efficacy of nurse-administered propofol sedation (NAPS) with those of PCS. METHODS: Eighty one patients were randomly assigned to two groups. All patients received meperidine 25 mg and propofol 40 mg as an initial dose for sedation. Patients in PCS group were subsequently infused with propofol 15 mg over 80 seconds through infusion pump whenever they required. Patients in NAPS group were injected with 10~20 mg propofol by nurse with supervision by endoscopist. The dosage of propofol, cardiopulmonary parameters, procedure time, sedation score, pain score, the patients' and endoscopists' satisfaction scores were assessed. RESULTS: With regard to blood pressure, pulse rate and oxygen saturation, serious complications were not observed. Especially, there was no significant difference of mean total dose between two groups (NAPS group and PCS group received 76.7+/-24.7 mg and 82.5+/-26.6 mg respectively). Pain score was higher in woman than in man (p=0.03). CONCLUSIONS: 1.2~1.5 mg/kg of propofol with small dose of opioid during colonoscopy was effective and safe. NAPS was more practical and useful method of sedation than PCS during colonoscopy.
Blood Pressure
;
Colonoscopy*
;
Female
;
Heart Rate
;
Humans
;
Infusion Pumps
;
Meperidine
;
Organization and Administration
;
Oxygen
;
Propofol*
10.A Case of Watermelon Stomach Treated by Hemigastrectomy.
Jin A KIM ; Ki Seok KIM ; Hyoung Sam KIM ; Sang Seok BAE ; Hee Bok CHAE ; Sei Jin YOUN ; Joung Muk LEEM ; Seok Jin OH ; Jae Hong CHOI ; Il Hun BAE ; Rohyun SUNG
Korean Journal of Gastrointestinal Endoscopy 2002;25(1):30-33
Gastric antral vascular ectasia (watermelon stomach) is a rare cause of chronic gastrointestinal bleeding, characterized endoscopically by a distinctive appearance of prominent red vascular folds traversing the gastric antrum and radiating to the pyloric sphincter; this appearance has been likened to the dark stripes on the surface of a watermelon. The etiology is unknown, and the usual presentations are iron-deficiency anemia and melena due to chronic gastric blood loss. Diagnosis is made by the characteristic endoscopic appearance and histologic findings. The treatment of choice is not known yet; surgical resection is curative method, but results from endoscopic treatment with heat probes or lasers are promising. We experienced a case of gastric antral vascular ectasia presenting with epigastric pain and melena in a 34-year-old female. The patient was treated with hemigastrectomy and Billroth II anastomosis.
Adult
;
Anemia, Iron-Deficiency
;
Citrullus*
;
Diagnosis
;
Female
;
Gastric Antral Vascular Ectasia*
;
Gastroenterostomy
;
Hemorrhage
;
Hot Temperature
;
Humans
;
Melena
;
Pyloric Antrum
;
Pylorus