Author investigated the deposition of immunoglobulins and complements in thc skin of 56 patients with 19 various dermatoses by direct immunofluorescent (DIF) staining. The biopsied specimens were quick-frozen(by dry ice-acetone) and stained with FITC conjugated antihuman immunoglobulin and complement after cutting in a cryostat at -20C~30C. (countinued...)
Complement System Proteins ; Dronabinol ; Fluorescein-5-isothiocyanate ; Humans ; Immunoglobulins ; Skin ; Skin Diseases*

In order to isolate the keratinophilic fungi from the soil, 7x10cm vinyl envelope were used in shich 10 rhomboid shaped holes (1x1cm) were made, so that when the human scalp hairs as baiting material were laid out straight in the envelope the keratinophilic fungi would be baited to the open part of the hairs in envilope in the 6 different places for 20 days in Kyungpood National University Hospital campus from April 1967 to September 1967. The author's newly improved baiting method with the hairs in the vinyl envelope was simple and the results were more satisfactory for isolating keratinophilic fungi compared with the original Vanvreuseghem's hair baiting method.
Fungi* ; Hair ; Humans ; Scalp ; Soil

Clofazimine (Lamprene or B668) is a phenazine congener that is used in leprosy, and the patients treated with the drug may develop red discoloration, dark brown pigmentation and ichthyotic skin. The authors observed 181 leprosy patients who had developecl discoloration, pigmentation and ichthyotic skin during clofazimine therapy, as well as disappearance of the pigmentation after stop of it. The results are summarized as follows: 1) Reddish discoloration was most frequently observed 2 weeks after beginning treatment in 66 patients (50. 4%), which showed earlier in the higher dosage grow up, 2) Dark brown pigmentation was iviost frequently observed 4 weeks after beginning treatment in 64 patients (48. 9%), which showed earlier in the higher dosage group. R) For histopathological evaluation of pigmentation, various ataining rnethods were used. On unstained frozen sections, yellow brown crystals were scattered in the dermis of both discolored and pigmented skin, and in H R E stain pigments, melanin were niarkedly increased in the epidermal basal layer and a, faint yellow brown. ghost was seen in dermis of pigmented skin. With fat stains using oil-red-0 and Sudan III, reddish amorphous materials were scattered in and around the cytoplasm of the macrophages c>f the pigmented skin. 4) Ichthyotic skin was observed in 97 patients, and most frequently 2 and R months after beginning treatment in 26 patients (26. 8%), but it was never observec1 in 34 of all patients in spite of a continuous intake of the drug for more than a. year. -countinue-
Clofazimine* ; Coloring Agents ; Cytoplasm ; Dermis ; Frozen Sections ; Humans ; Leprosy* ; Macrophages ; Melanins ; Pigmentation ; Skin ; Sudan

Fibronectin(FN) is a major component of the extracellular matrix and is able to bind to cells and other components of the matrix. Although the cell producing the largest amounts of fibronectin is fibroblast, the pro duct,ion on FN also has been described in cultured keratinocyt,e and epithelial tumor cells of basal cell carcinoma(BCC). Recently, functional role nf FN in relation to biologic behavior of BCC and squamous cell carcinoma(SCC) is open to speculation. The authors investigated the localization of FN in the lesional skin of 5 cases of BCC, 4 of SCC, 5 of psoriasis and 5 normal skin using direct immunofluorescence technique with antifibronectin antibody to find out the production of FN in keratinocytes and tumor cells. 1. In the skin of BCC, FN was presented in a thick, linear depositions along the margin of tumor lobules in all cases, and as fillamentous deposits or scattered points in the nest of tumor cells in 3 cases. 2. In the skin of SCC, FN was presented in a thin, coarse depositions around the margin of tumor but not presented within the tumor nest in all cases. R. In psoriasis, FN was observed in horney layer and upper part of epidermis. It was presented more abundantly in dermo-epidermal junction, papillary and reticular dermis than in normal skin. 4. In normal skin, FN was absent in the epidermis but presented in dermo-epidermal junction and blood vessel wall of upper dermis, the amount of FN was decreased from papillary to lower dermis.
Blood Vessels ; Carcinoma, Basal Cell* ; Carcinoma, Squamous Cell ; Dermis ; Epidermis ; Extracellular Matrix ; Fibroblasts ; Fibronectins* ; Fluorescent Antibody Technique, Direct ; Keratinocytes ; Psoriasis* ; Skin*

Darier's disease is a congenital dyskeratosis and is as an autosomal dominant reait but a case with a family history is relatively rare and only 2 such cases have been reported in Korea. We present typical Darier's disease occuring in a mother and her 2 daughters. A 45 year-old female has shown muitiple, confluent, dirty verrucous keratotic papules on the seborrheic area and whitish papules on the sotf palate since the age of 7 year-ole. Histologically there, are corps ronds, grains and suprabssal lacunae in the epidermis.
Edible Grain ; Darier Disease* ; Epidermis ; Female ; Humans ; Korea ; Middle Aged ; Mothers ; Nuclear Family ; Palate

The lupus band test(LHT) has been proposed as a diagnostic test for lupus erythematosus(LE), differentiating discoid I E from systemic LE and also has prognostic values. For the better understanding of relation between LBT and prognosis in SLE. a study was carried out in 32 SLE patients with skin lesions. Immunofluorescent study was done with biopsies taken from involved skin and uninvolved skin of patients with SLE. The results were as follows . 1) Incidences of a positive LBT were 84% in both involved and uninvolved skin and IgM was most frequently deposited(66% & 72%) in both, whereas IgA was least frequently deposited(34% & 19%) in both. 2) Deposits in DEJ of IgG, IgM, IgA and C were found with approximately same frequency in patients with different clinical manifestations and different laboratory findings. 3) Incidences of positive LBT in patients with renal disease were approximately same as in patients without renal disease. And deposits of all clssses of immuno glcbulins were found with about the same frequency in these two groups. 4) A positive LBT composed of IgG alone or IgG and other Igs were associated with an increase of anemia, ANA, anti-DNA and hypocomplementemia. Ten renal biopsies showed diffuse proliferative glomerulonephritis(GN) in focal proliferative GN in 3 and membranous CiN in 2 and no correlation to immune deposits.
Anemia ; Biopsy ; Diagnostic Tests, Routine ; Humans ; Immunoglobulin A ; Immunoglobulin G ; Immunoglobulin M ; Incidence ; Lupus Erythematosus, Systemic* ; Prognosis ; Skin

There are many reports about the deposition of immunoglobulins and complement by direct immunofluorescence(DIF) in the lesion of scabies. The authors investigated the immunoglobulins and C, for the purpose of studying the immunologic reactivity of the host to scabies mites by DIF. The results are summerized as follows: Total number of the cases investigated were 20 (18 males, 2 females), of whom 11 were in their third decade of age. The clinical forms of the tested lesions were papular type (7 cases), nodular type (12 cases), and Norwegian type (1 case). The durations of the patients were mostly within 2 months, but was 9 months in 1 case of nodular type, Deposition of immunoglobulin and C, was demonstrated by DIF in 10 of 12 cases (83. 3%) of nodular type, and 1 of 7 cases (14. 3%) of papular type. The Norwegian type showed negative results. There seemed to be no relation between the duration of scabies and positive rates. Of 20 cases tested, immunoglobulin and C, were deposited in the vascular wall in 11 lesions (55%), and at the dermoepidermal junction in 3 lesions (15%). While in 1 of 7 cases of papular type both IgM and C, were observed, IgM and C, were noted in 7 and 9, respectively, in 12 cases of nodular type.
Complement System Proteins ; Humans ; Immunoglobulin M ; Immunoglobulins ; Male ; Mites ; Scabies*

Fifteen hundred seventy-one leprosy patients registered in the leprosy Mission Hospital from Mar. 1957 to Sep. 1980 were investigated for dapsone resistance by clinical and bacteriological aspects. The results are as follows: There were 29 suspected dapsone-resistant cases out of a total 1571 registered leprosy patients (1. 85%), 26 of 650 of lepromatous type(4. 00%), and 3 of 164 of borderline type (l. 83%). In 1969, we observed the first case if suspected dapsone-resistant leprosy. Since then, suspected dapsone-resistant cases increased and we observed as many as 8 cases in 1977. But frequency of cases has decreased since 1978. (countinued..)
Dapsone ; Humans ; Leprosy* ; Missions and Missionaries

In order to study immunological responses in psoriatic lesions, direct immunofluorescence(DIF) findings in stratum corneum of psoriatic lesions are compared with histopathological changes. The results are summerized as follows: 1) Deposition of Igs or C 3 in stratum corneum of psoriatic lesions was demonstrated in 14(56%) of 25 psoriasis patients. IgG was present in 40% IgA in 48%, IgM in 24%, and C 3 in 32%. However, DIF findings are negative in the str,atum corneum in the uninvolved skin of psoriasiis. In control groups of 12 cases of various skin diseases, DIF findings were negative in the stratum corneum except 1 case of a patient with acne rosacea. 2)DIF findings in 22 cases of histopathologic changes of psoriatic lesions were found in 50% of hyperkeratosis in 48% of parakeratosis in 50% of Munro microabscess, in 50%, of spongiform pustule, and in 57% of the absence of stratum granulosum: ie, about only 50% were positive in DIF results. Histopathologic changes in 11 cases of positive DIF finding in psoriatic lesions were hyperkeratosis in 100%, parakeratosis in 91%, Munro microabscess in 64%, spongiform pustule in 55%, and the absence of stratum granulosum in 73%.
Humans ; Immunoglobulin A ; Immunoglobulin G ; Immunoglobulin M ; Parakeratosis ; Psoriasis ; Rosacea ; Skin ; Skin Diseases

This study was done to observe the statistical difference on bullous skin disease patients (pemphigus, bullous pemphigoid, dermatitis herpetiformis, epidermolysis bullosa) who visited the department of dermatology, Kyungpook National University Hospital,from January 1966 to Deceniber 1985, For those 24 cases which tested positive under direct immunofluorescent (DIF) testing, agreement percentage of histopathologic diagnosis was compared. The results were summarized as follows : 1) 51 of 45,091 new outpatients (0.113%) were bullous skin disease patients. 2) By sex, age and monthly distribution, there was no significant difference, though epidermolysis bullosa was seen for the most part in those under 10 years of age. 3) By individual disease distribution, the pemphigus cases numbered 8, of which most cases were within the 30~60 year age group. Bullous pemphigoid cases numbered 14, of which most cases were over 50 years of age. Female patients were 2.5 times more common than male patients. Dermatitis herpetiformis cases numbered 20, and were relatively diminished in recent years. The peak age group was the 3rd decade. Epidermolysis bullosa numbered 9 cases. 4) The compatibility of the histopathologic diagnosis to the DIF diagnosis in 24 DIF positive cases indicated that of DIF positive pemphigus the histopathologic diagnrsis agreed with the DIF diagnosis in 75%, of bullous pemphigoid, 58% and of dermatitis herpetiformis, 50%.
Dermatitis Herpetiformis ; Dermatology ; Diagnosis ; Epidermolysis Bullosa ; Female ; Gyeongsangbuk-do ; Humans ; Male ; Outpatients ; Pemphigoid, Bullous ; Pemphigus ; Skin Diseases, Vesiculobullous* ; Statistics as Topic*