Objective:To observe the change of serum homocysteine (Hcy),macrophage colony stimulating factor (M-CSF),uric acid (UA),platelet related indexes and trace element levels in coronary heart disease (CHD)pa-tients with angina pectoris.Methods:A total of 58 CHD patients with angina pectoris treated in our hospital from Oct 2010 to Oct 2013 were selected as angina pectoris group,another 58 healthy subjects in same period were en-rolled as healthy control group.Levels of Hcy,M-CSF,UA,platelet related indexes [platelet distribution width (PDW),CD61,CD63]and trace elements were compared between angina pectoris group and healthy control group.Results:Compared with healthy control group,there were significant rise in serum levels of platelet related indexes,Hcy,M-CSF,UA,Zn and Fe,and significant reduction in serum Mn level in angina pectoris group,P <0.05 all;compared with patients with stable angina pectoris,there were significant rise in serum levels of PDW [(13.37±1.61)vs.(17.86±1.99)],CD61 [(45.26±5.11)vs.(58.40±7.20)],CD63 [(14.05±1.96)vs. (18.78±2.95)],Hcy [(13.47±1.52)mmol/L vs.(21.96±2.23)mmol/L],M-CSF [(289.73±31.68)pg/ml vs. (401.56±48.96)pg/ml],UA [(287.95±36.49)μmol/L vs.(373.45±45.26)μmol/L],Zn [(15.76± 1.84)μmol/L vs.(19.55±1.98)μmol/L]and Fe [(12.68±1.50)mmol/L vs.(15.71±1.83)mmol/L],and significant reduction in serum Mn level [(2.21±0.34)μmol/L vs.(1.60±0.21)μmol/L]in patients with unstable angina pec-toris (P <0.05 all).Along with number of coronary diseased vessels increased,serum levels of above indexes signifi-cantly rose except serum Mn level,P <0.05 all.Conclusion:The serum Hcy,M-CSF,UA,platelet related indexes and most trace elements levels significantly rise in CHD patients with angina pectoris,and the abnormal degree is greatly influenced by the angina pectoris type and number of coronary diseased vessels.

Objective To investigate the value of laparoscopic cholecystectomy through superior margin of public symphysis approach.Methods The clinical data of 72 patients with benign gallbladder diseases who were admitted to the Lihuili Hospital from December 2012 to August 2013 were retrospectively analyzed.There were 54 patients with cholecystolithiasis and 18 with gallbladder polyps.Thirty patients received laparoscopic cholecystectomy through the superior margin of public symphysis approach (new method group).Forty-two patients who received transumbilical single-port laparoscopic cholecystectomy were in the control group.The operation time,intraoperative blood loss,duration of postoperative hospital stay,degree of postoperative pain and cosmetic effect of the 2 groups were compared.Patients were followed up via out-patient examination and phone call till December 2013.Data were analyzed using the t test or chi-square test.Results Two patients in the new method group and 1 patient in the control group were converted to receive traditional laparoscopic cholecystectomy.The operation time,intraoperative blood loss,duration of postoperative hospital stay,scores of satisfaction with the incision and degree of postoperative pain were (28 ± 3) minutes,(23 ± 10) mL,(2.0 ± 0.5) days,4.3 ± 0.5 and 5.8 ± 0.8 in the new method group,and (39±4)minutes,(24±l0)mL,(2.0±0.6)days,3.9±0.5 and 5.9±0.9 in the control group.There were significant differences in the operation time and score of satisfaction with the incision between the 2 groups were detected (t =10.032,2.423,P ＜ 0.05),while no significant differences in the volume of intraoperative blood loss,duration of postoperative hospital stay and degree of pain between the 2 groups (t =1.021,0.000,1.760,P ＞ 0.05).All the patients were recovered,and were administered with semi-fluid food at postoperative day 2.No bleeding,bile leakage,incisional infection occurred,and no patient died perioperatively.Analgesics were not needed in the 2 groups.All the patients were followed up for 1-6 months.Patients were satisfied with the cosmetic appearance of the incision,and no inflammation,pain and infection of the incision occurred.Conclusions Laparoscopic cholecystectomy through the superior margin of public symphysis approach is safe and feasible,with the advantages of short operation time,cosmetic appearance of incision and easy manipulation.

Recent years a large number of researches and studies of modern rehabilitation medicine have made great progress in various fields,such as basic theory,evaluation,treatment,institution,and network.The objective of this paper is to outline these advances,which could be benefit for medical affairs in rehabilitation of cerebrovascular diseases.

This paper reviewed the development of the horseback riding exercise machine in Japan based on the theory of therapeutic riding, imitating the saddle movement by using data collected three dimensional from living horse, involving virtual reality technique, 6-axes parallel mechanism and motion capturing technique. It is effective to improve muscular strength and insulin resistance in elderly people. Mechanical horseback riding is hopeful to be used in clinical approach to improve the quality of life in elderly people in the future.

Adult ; Female ; Humans ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; genetics ; Translocation, Genetic

BACKGROUND:The combination of anterior and posterior approaches for severe cervical intervertebral disk herniation, posterior longitudinal ligament calcification and ligament flava hyperplasia can completely reduce compression. Simultaneously, cage implantation and anterior plate fixation partial y recover vertebral interspace and physiological curvature of cervical vertebra.
OBJECTIVE:To compare the effects of cervical posterior single-door laminoplasty for cervical spondylosis and anterior cage-assisted fusion on curvature, stability and axial symptoms after treatment.
METHODS:Clinical data of 50 patients, who received surgical treatment for cervical spondylosis, were retrospectively analyzed. 22 cases underwent I-stage spinal decompression by the combination of anterior and posterior approaches (anterior fusion cage+steel plate fixation) (combination group). 28 cases underwent cervical posterior single-door laminoplasty (posterior windowing rivet fixation) (posterior approach group). They were fol owed up for 6 to 24 months. The recovery of neurological function, incidence of axial symptoms, cervical curvature index, and slipping degree of affected intervertebral segments were analyzed statistical y.
RESULTS AND CONCLUSION:The neurological functions were greatly improved according to Japanese Orthopaedic Association in both groups. Incidence of axial symptoms was relatively low in the combination group. Cervical curvature index was obviously improved in the combination group compared with the posterior approach group. The slipping degree of affected intervertebral segments was relatively greater in the posterior approach group than that in the combination group. Results suggested that fusion cage, anterior steel plate and posterior rivet for spinal decompression in I-stage combination of anterior and posterior approaches can effectively maintain cervical physiological curvature and the balance of sagittal level, reduce incidence of axial symptoms, and play a key effect on keeping posttreatment long-period cervical stability.

Objective To investigate the relationship between the C161T polymorphisms in peroxisome proliferators-activated receptor γ (PPARγ) gene and atherothrombotic infarction (ATI) in a Chinese Han population.Methods The patients with atherothrombotic infarction and controls,who without histories of stroke,transient ischemic attack and myocardial infarction were enrolled.Genotypes of the PPARγ C161T polymorphisms were determined using the polymerase chain reaction-restriction fragment length polymorphism.The frequencies of genotype and allele in patients with ATI and controls were compared.Results A total of 112 ATI patients and 112 controls were enrolled.There were no significant differences in the age (67.9± 11.73 years vs.66.5 ± 10.35 years; t =0.386,P =0.701) and the proportion of male (61.61％ vs.58.04％ ;x2 =0.297,P =0.586) in the ATI group and the control group.The proportions of hypertension (59.82％ vs.44.62％ ;x2 =5.171,P =0.023),type 2 diabetes mellitus (26.79％ vs.9.82％;x2 =10.778,P =0.001) in the ATI group were significantly higher than those in the control group.Body mass index (25.13±1.86 kg/m2 vs.24.11 ± 1.81 kg/m2; t =3.543,P=0.001),systolic pressure (158.84 ± 20.15 mm Hg vs.135.82 ± 19.58 mm Hg; t =7.350,P =0.000),diastolic pressure (76.90 ± 13.64 mm Hg vs.68.90 ± 8.52 mm Hg; t =4.374,P =0.000) and the level of fasting blood glucose (6.523 ± 2.831 mmol/L vs.5.706 ± 2.177 mmol/L; t =2.026,P =0.044) in the ATI group were significantly higher than those in the control group.The genotype frequencies of CC,CT and TT in the ATI group were 77.7％,17.0％ and 5.4％,respectively,and in the control group were 69.6％,22.3％ and 8.0％,respectively.There were no significant difference between the two groups (x2 =1.909,P =0.385).The allele frequencies of C and T in the ATI group were 86.2％ and 13.8％,respectively,and in the control group were 80.8％ and 19.2％,respectively.There were no significant difference between the two groups (x2 =2.331,P =0.127).Conclusions PPARγ C161T polymorphisms may be not associated with the onset of ATI in a Chinese Han population.

AIM: Firstly to evaluate the changes of corneal sensation and its effect to dry eye syndrome after laser in situ keratomileusis (LASIK); secondly to evaluate the relation between the changes of corneal sensation and the laser ablation depth. ·METHODS: 30 patients (60 eyes) had bilateral LASIK with a superior-hinged flap. Every patient underwent preoperative and postoperative (after one week, one month and three months) evaluations, including corneal sensation, Schirmer's basic tear secretion test, tear breakup time (TBUT), corneal fluorescein stainting, and a questionnaire evaluating dry eye obtained from each patient.·RESULTS: Compared with preoperative level, after one week and one month, the difference of corneal sensation was very significant (P＜ 0.01); after three months, there was no statistical difference (P ＞ 0.05). After one week, there was no statistical difference (P ＞ 0.05) in the subjective dry eye symptoms, after one month, the difference was significant(P=0.025 ＜0.05) and after three months, the difference was very significant (P=0.001 ＜ 0.01). Compared with preoperative level, the basic tear secretion decreased significantly after one week and one month(P＜ 0.01), it did not return to the baseline level after three months (P ＜ 0.01). Tear breakup time decreased significantly after the surgery, and after three months, there was still statistical difference compared with preoperative level (P ＜ 0.01); with cornea fluorescence staining, we found corneal epithelial defect was most serious after one month after LASIK, and did not return to the baseline level after three months (P ＜ 0.01). Linear regression analysis and relevant were used to evaluate the relation between the changes of corneal sensation and the laser ablation depth (r=0.798, P ＜ 0.01). ·CONCLUSION: The central corneal sesation decreased noticeable after LASIK, and it recovered three months postoperatively, but the indicators of dry eye syndrome did not return to their preoperative levels following it. We have found that the decreasation of corneal sensation had positive correlation with the laser ablation depth.

Objective To analyze postoperative complications of surgical therapy for deep vein thrombosis (DVT) of the lower extremity. Method From January 2001 to January 2008 vena cava filters were placed in 171 DVT cases before surgery. Fogarty catheter (73 cases), Amplatz thrombectomy device (ATD) (55 cases), Acolysis ultrasound ablation(43 cases) were used to extract the thrombi in iliac and proximal femoral vein. The full extraction of thrombi in distal end was facilitated by compressing and massaging the legs in all cases. Iliac venous stenosis or occlusion was managed by interventional therapy, and temporary femoral arteriovenous fistula were carried out routinly. Result Operations were successful in 157 cases(70 cases in Fogarty group, 52 in ATD group,35 in Acolysis ultrasound ablation group), and failed in 14 cases (3 cases in Fogarty group, 3 in ATD group, 8 in Acolysis ultrasound ablation group). Permanent,retrievable and temporary vena cava filters were placed in 51,32 and 88 cases respectively. Stenosis or occulsions of the iliac vein was found in 143 cases and stents were placed after balloon dilatation in 41 cases. During the operation, residual thrombi was found in 80 cases. Vessel perforation and rupture were complicated in 14 cases, thrombosis adhering in eava vein filters in 18 cases. There was no mortality and no pulmonary thromboembolism. During the follow-up, we found iliae vein restenosis in 21 cases, thrombosis recurrence in 36 cases, stents displacement in 6 cases, and stents fracture in 2 cases. Conclusion Surgical therapy is effective for DVT of the lower extremities.

In recent years , fungal biofilms related infection have become an increasingly important clinical problem .Many clinically important fungi can form biofilms , including:Candida albicans , Cryptococcus neoformans , Rhodotorula species , Aspergillus fumigatus, Malassezia pachydermatis, Histoplasma capsulatum.Many chronic persistent infections are associated with the fungal bio-film formation.The developmental phases of fungal biofilms are complicated , including adhesion, colonisation, maturation and dispers-al, which are governed by many genes .This review discusses clinical impact of biofilm formation of the common human pathogenic fun -gi, biofilm structure and the molecular mechanisms during the biofilm formation process .