No abstract available.
Irritable Bowel Syndrome*

No abstract available.
Irritable Bowel Syndrome*

An improved protocol for in situ hybridization(ISH) to routinely processed, paraffin-embedded tissue sections from lung carcinoma is presented. For this study, DNA probes for alpha-satellite chromosome 7 and 17 were used. The protocol to detect numerical chromosome aberrations involved treatment of sections with 1 M sodium thiocyanate prior to pepsin digestion, resulting in reproducible ISH reactions. The effect of avidin-biotin detection system. Four layer avidin methods and triple biotin methods, using avidin-PO, goat antiavidin, biotinylated antigoat IgG, avidin-PO or anti-biotin, biotinylated antirabbit IgG, avidin-PO, markedly enhanced the intensity of positive signals. More than 80% of the tumor and stromal cells showed distinct chromosome hybridization signals in 6 micrometer-thick sections. Lung carcinoma cells showed multiple chromosome signals(2~5 spots), contrasted by one or two signals in the stromal cells in the same section. These results suggest that chromosome polysomy can be reliably detected in tissue sections using in situ hybridization. This capability will prove to be an important tool for determining the underlying genetic basis for tumor development, tissue phenotype heterogeneity and progression by allowing genetic determination to be made on paraffin-embedded tissue sections where tumor histologic architecture is preserved.

The author investigated 100 hypertensive patients (199 eyes) on their brachial blood pressure, ophthalmic blood pressure, Fritz's index, sclerotic changes in retinal vessels, and P.S.P. test. The vascular changes of the retina were classified according to the Keith-Wagener scheme, and the sclerotic changes were represented by the point system. For the evaluation of circulatory status of the carotid system, r(s)/r(d)-carotis ratio and cerebrovascular resistance (CVR) were also calculated. The relationships between these data were studied and their results were as follows: (1) There was a linear correlation between mean ophthalmic blood pressure and mean brachial blood pressure, the correlation coefficient being plus 0.83. (2) There was also a linear relationship between fundus changes and mean ophthalmic blood pressure. The more advanced the grade of Keith-Wagener classification and the sclerotic changes, the higher the mean ophthalmic blood pressure. The rise of the mean ophthalmic blood pressure as more marked in K-W grade III, IV, and in grade IV sclerosis. (3) There was a close relationship between P.S.P. test, mean ophthalmic blood pressure and brachial blood pressure. The renal function was disturbed in patients with higher mean ophthalmic blood pressure. (4) The decrease of r(s)/r(d) carotis was always accompanied by the increase of Fritz's index, severity of sclerotic changes in the retina and advancement of the grade of K-W classification. (5) In patients with normal P.S.P. value, there was no single case of advanced sclerosis (more than 10 points of sclerosis). r(s)/r(d) carotis ratio was generally over 1.0. In patients with low P.S.P. value, however, the retinal vascular sclerosis shoshowed more marked changes, and r(s)/r(d) carotis ratio failed below 1.0. (6) Generally, there was a tendency of the increase in the CVR in accordance with the increase in Fritz's index and sclerotic changes. Moreover, functional disturbance of the kidney was usually associated with higher CVR. (7) The author proposed the possibilities that CVR value more than 0.5 or r(s)/r(d)-carotis ratio below 1.0 might represent the advanced sign of vascular sclerosis, disturbance of kidney function.
Blood Pressure ; Classification ; Humans ; Kidney ; Retina ; Retinal Vessels ; Retinaldehyde ; Sclerosis*

The mineral contents of the adult Clonorchis sinensis from rabbits and human were measured, and its qualitative and semi-quantitative analyses were studied with 3.4 meter Ebert-Grating spectrograph, and its quantitative analyses were studied with Beckman DU spectrophotometer. The adult Clonorchis sinensis used in this study were divided into two groups, the first group was collected from the bile passage of the man (C. sinensis from man), the second group was collected from the bile passage of the rabbit(C. sinensis from rabbit). Simultaneously, spectrographic and photometric analyses were also performed on the adult worms of Paragonimus westermani. Furthermore, comparative spectrographic analyses of the trace elements were carried out on the C. sinensis from man, C. sinensis from rabbit, Fasciola hepatica, Eurytrema pancreaticum, and Paragonimus westermani, and the approximate contents of the trace elements of the above trematodes were compared with that of their host tissues and biles. The results obtained were as follows: In the spectrographic analyses of C. sinensis from man, sodium, phosphorus, potassium, copper, calcium, aluminum, iron, and magnesium were detected predominantly, and zinc, titanium, silicone, lead, manganese, barium, cromium, molybdenum, and silver were detected as trace elements. In the differences of level of the minerals in these two C. sinensis, copper level of C. sinensis from man was higher than that of C. sinensis from rabbit, while calcium level of the former worm was higher than that of the latter worm. The trace level of lead, molybdenum, and silver were acertained in the former, but latter were not detected . The contents of the minerals showed the characteristic features in each trematodes: the mineral of flukes in each host were much more than that of the others; such as copper in C. sinensis from man, calcium in the C. sinensis from rabbit, and silicone in the P. westermani. The vanadium was detected in the F. hepatica, E. pancreaticum, and P. westermani, while the other flukes were not detected. In the comparative spectrographic analyses of the trace elements among the trematodes and their host tissues and biles, the minerals which detected from flukes were also found in their tissues and biles of their host. But the mineral levels of C. sinensis from man, F. hepatica, E. pancreaticum, and P. westermani were higher than that of their host tissues and biles, except the C. sinensis from rabbit.
parasitology ; helminth ; trematoda ; C. sinensis ; rabbit ; Fasciola hepatica ; Eurytrema pancreaticum ; Paragonimus westermani ; biochemistry ; sodium ; phosphorus ; potassium ; copper ; calcium ; aluminum ; iron ; magnesium ; zinc ; titanium ; silicone ; lead ; manganese ; barium ; cromium ; molybdenum ; silver ; vanadium

Building-related illness is an increasingly common problem. The disease fall into two categories : those that have an identifiable cause-such as legionellosis, humidifier fever, and conditions resulting from exposure to known substances such as asbestos, lead in paint, formaldehyde, etc-and those that have no readily identifiable cause but can be described only by a group of symptoms known as sick building syndrome (SBS). Although objective physiologic abnormalities are generally not found and permanent sequelae are rare, the symptoms of SBS can be uncomfortable, even disabling, and whole workplaces may be rendered non-functional. In assessment of patients with SBS complaints, specific building-related illnesses should be ruled out by history or physical examination. On-site assessment of buildings is extremely useful. Symptoms of non-specific building-related illnesses are common ; their heterogeneity suggests that they do not represent a single disorder. Although there is little convincing, direct evidence to implicate specific causative agents, there is sufficient indirect evidence to support a number of recommendations. For example, it seems prudent to maintain an outdoor-air supply of more than 10 liters per second per person ; to select the building materials, furnishings, and equipments that are least likely to release pollutants such as formaldehyde or volatile organic compounds ; to ensure proper maintenance and cleaning ; and to avoid materials that may act as substrates for the proliferation of microbes or dust mites.
Asbestos ; Construction Materials ; Dust ; Fever ; Formaldehyde ; Humans ; Humidifiers ; Legionellosis ; Mites ; Paint ; Physical Examination ; Population Characteristics ; Sick Building Syndrome ; Volatile Organic Compounds

No abstract available.
Small Cell Lung Carcinoma*

No abstract available.
Dementia* ; Primary Health Care*

No abstract available.
Korea*

This is a statistical observation based on data of deaths occurred in the first army group(1A) of Republic of Korea Army (ROKA) and data brought to Wonju Army Hospital for medicolegal autopsy by all of Military Police Corps (MPC) in 1A during the year of 1996. The report aims to reveal facts on various causes of death of military members in attempt to analyse 70 cases statistically and differences between military members and civillians. The following are the summary of results ; 1. The total numbers of death in 1A were 70 cases and autopsy rate was 17.1%(12 cases). All cases were males. 2. The violent deaths were 60 cases(85.7%) and the natural deaths were 10 cases(14.3%). 3. The percentage of deaths of soldiers was 81.4%(the most), sergeants was 8.6%, officers was 5.7%, others was 4.3%. The group of the private (25.7%) and the private first class (25.7%) was the leading group of death by the rank. 4. For deaths due to injuries, the percentage of deaths due to gunshut injury was 51.2%, traffic accident was 30.1%, explosion of bomb was 7.0%, fall was 7.0% and blunt injury was 4.7%. 6. For the natural deaths, 10 cases were recorded and death due to neoplasm was taking the most of the total percentage with 40%. 7. For the violent deaths, the percentage of accidental death was 42.4%, suicide was 40.7% and homicide was 16.9%. These results suggest that suicide by firearm and traffic accident including military owned vehicle could be the major cause of death in army, and intensive management for group of the private and the private first class could be important to reduce the number of death.
Accidents, Traffic ; Autopsy ; Bombs ; Cause of Death ; Explosions ; Gangwon-do ; Homicide ; Hospitals, Military ; Humans ; Male ; Military Personnel ; Police ; Republic of Korea ; Suicide ; Wounds, Nonpenetrating