Using immunohistochemical technique, we observed time-sequentially to T and B lymphocyte in the ciliary body of the endotoxin induced uveitis in rat. The primary antibodies for immunohistochemical stain of the lymphocyte were IgM heavy chain, IgG heavy chain, T suppressor/cytotoxic (CD8) and T helper/macrophage (CD4) was performed in the frozen section. The intensity of immunohistochemical reaction for surface IgM positive cells increased from 12hours and reached most strong intensities at 24hours after endotoxin injection and then decreased rapidly, but the intensity for surface IgG positive cells increased from 48hours and sustained 96hours. There were no significant changes of intensity of immunohistochemical reaction for T cells except slightly increased T suppressor/cytotoxic cells (CD8).
Animals ; Antibodies ; Ciliary Body ; Frozen Sections ; Immunoglobulin G ; Immunoglobulin M ; Lymphocytes ; Rats* ; T-Lymphocytes ; Uveitis*

No abstract available.
Bronchial Arteries* ; Hemoptysis*

From March, 1988 to August, 1989, the authors performed nasolacrimal duct reconstruction with Pyrex glass tube without bony perforation in 26 eyes of 25 patients who has chronic dacryocystitis with nasolacrinal duct obstruction. This method is implantation of permanent indwelling pyrex glasstube from base of the sac through the interosseous canal into the vault of inferior nasal meatus and there are several advantages in this method; The technique is more simple and less traumatic than other dacryocystorhinostomy, tear drainage inferioly prevents pooling of tear in inferior portion of lacrimal sac after surgery, normal anatomic relationships are retained and less postoperative care is required. The results were as follows: 1. Success was achieved in 24 of 26 cases including partial success. 2. Postoperative complications were developed that hypertrophic scar 2 cases, superficial punctate keratitis 1 case, wound infection 1 case, nasal bleeding 1 case.
Cicatrix, Hypertrophic ; Dacryocystitis ; Dacryocystorhinostomy ; Drainage ; Epistaxis ; Glass* ; Humans ; Keratitis ; Nasolacrimal Duct* ; Postoperative Care ; Postoperative Complications ; Tears ; Wound Infection

The Kniest syndrome is characterized by disproportionate dwarfism and Kyphoscoliosis which may be associated with flat facies with prominent eyes, cleft palate, hearing loss, myopia and limited joint motion. The skeletal abnormalities are recognizable at birth with shortening and deformity of the extremities and stiff joints. Marked lumbar lordosis and kyphoscoliosis develop in childhood, resulting in disproportionate shortening of the trunk. We experienced a case of kniest syndrome, confirmed by clinical features, radiological features, and histological examination of cartilage. A brief review of the related literature is presented.
Animals ; Cartilage ; Cleft Palate ; Congenital Abnormalities ; Dwarfism ; Extremities ; Facies ; Hearing Loss ; Joints ; Lordosis ; Myopia ; Parturition

No abstract available.
Aging* ; Endocrine Cells* ; Neurons*

The effects of Equiseti Herba Water-heating extract on the kidey & liver of rats were studied in 25 male Sprague-Dawley rats evenly divided into five groups, i.e., 5 rats each. The group I, which served as control and the group II, III, IV, were fed 300 mg/kg, 1000 mg/kg of Eqiseti Herba water-heating extract daily for one week, respectively. The group V was fed 1000 mg/kg of Equiseti Herba extract daily for two weeks. The effect of Equiseti Herba on the kidney and liver were determined by the measurements of the serum levels of BUN, creatinine, calcium, phosporus, sodium and potassium, total protein, albumin, transaminase (GOT & GPT) and cholesterol and 24hrs urine volume and GFR(ml/min), as well as by light microscopic and ultrastructural examinations of kidney and light microscopic examinations of liver in rats. The results were as follows; The groups II, III, IV were slightly higher than the control group on serum creatinine and potassium levels, but the group V was declined on serum creatinine and potassium levels, and 24hrs urine volume in spite of increased GFR. Histologically there was no remarkable changes in groups compared with the group I, except mild vacuolization of proximal convoluted tubule in group IV and V. These results suggested that equiseti Herba water-heating extract in rats induced reversible tubular changes on the kidney of rats. But the groups administrated by Equiseti Herba water-heating extract were not significantly higher than the control group on liver function test. Histologically there was no remarkable changes in groups II, III, IV & V compared with the group I.
Animals ; Calcium ; Cholesterol ; Creatinine ; Humans ; Kidney* ; Liver Function Tests ; Liver* ; Male ; Potassium ; Rats* ; Rats, Sprague-Dawley ; Sodium

PURPOSE: Many patients who intentionally poison themselves are reported to have underlying mental problems and to repeat the attempted suicidal poisoning. As a first step to prevent this regretful complication, we looked into the present status of psychiatric follow-up of the patients who intentionally poisoned themselves. METHODS: Using a chart review and telephone contact, we analyzed the psychiatric follow-up rate, the underlying psychiatric illness, the number of suicidal attempts, and the reasons for no follow-up for adult patients who intentionally poisoned themselves and who visited the Emergency Department of Severance Hospital from Mar. 2003 to Feb. 2004 with chart review, telephone contact. RESULTS: Forty-nine of 119 suicide patients (41.2%) chose poisoning as a means of suicide. The results of emergency treatment of those 119 patients were 4 deaths during treatment, 36 admissions (15 psychiatry, 21 others), and 79 discharges including 5 transfers. The most common underlying psychiatric problem based on 103 initial psychiatric interviews was depressive disorder in 52(50.5%) patients. Not counting the 19 deaths and psychiatry admissions, 26 patients received psychiatric follow-up during admission or after discharge, and the remaining 74 patients were subjected to telephone surveys. In the telephone surveys, only 53 patients were contacted; 25 patients declined to answer the questions and out of the 28 patients who answered them, 5 patients had received psychiatric follow-up at other hospitals. The most common reason given by the other 23 patients for no follow-up was that it was not necessary (39.1%, 9/23). The overall follow-up rate was 39.2% (31/79). Of the 48 patients who answered the question on repeated suicide attempts, 3 patients had repeatedly attempted suicide. CONCLUSION: To prevent further suicide attempts and resultant deaths in cases of patients who intentionally poisoned themselves, we urgently need an integrated psychiatric follow- up care program that is activated through a collaborative effort before discharge from the emergency department.
Adult ; Depressive Disorder ; Emergency Service, Hospital ; Emergency Treatment ; Follow-Up Studies* ; Humans ; Intention ; Poisoning* ; Suicide ; Suicide, Attempted ; Telephone

Doxorubicin has characteristic chemomyectomy effect of the eyelid without disturbing other eyelid structures, but the major side effect of doxorubicin is the potential for eyelid skin injury as a result of the drug's toxicity in both animal and clinical studies. Verapamil may be used to reduce the dose of doxorubicin and the number of injections that would amplify the toxic effects of doxorubicin. This study was performed to determine whether there is an increase in the toxic effect of the doxorubicin as a result of verapamil pretreatment of the muscle. After 0.5mg, 1.0mg, and 2.0mg doxorubicin was injected in lower eyelids of each group, and equal dose of doxorubicin was injected fo11owing 1.0mg of verapamil injection in lower eyelid of each group, muscle cell loss were measured by light microscopy and side effect was observed. In verapamil and doxorubicin injection group, there was significant differences in the amount of preseptal muscle and even in the pretarsal muscle than the doxorubicin injection group in all doxorubicin doses. Verapamil, injected with a range of doses of doxorubicin, caused suhstantia11y increased muscle loss in the eyelid, compared with injection of doxorubicin alone. Skin ulceration, entropion or ectropion were not visible. Clinically, verapamil cotreatment might be useful to decrease the dose of doxorubicin injected and/or the total number of injections.
Animals ; Doxorubicin* ; Ectropion ; Entropion ; Eyelids ; Microscopy ; Muscle Cells ; Skin ; Skin Ulcer ; Verapamil*

PURPOSE: Cystic neoplasms of the pancreas are rare tumors that occurs in approximately 10~15% of all cystic lesions of the pancreas. A recent trend has been to resect all cystic neoplasms, without any attempt to preoperatively determine the exact histologic subtype. METHODS: We retrospectively reviewed the clinical records of fifteen patients with cystic neoplasms and intraductal papillary mucinous neoplasms of the pancreas who were treated surgically between January, 1991 and May, 2001. RESULTS: Patient ages ranged from 14 to 69 years with a mean of 53.4. Sixty percent of patients were women. There were 4 cases of mucinous cystic neoplasms, 7 solid pseudopapillary tumors, 3 intraductal papillary mucinous neoplasms, and 1 serous cystadenoma. The most prominant symptom was an abdominal mass. Pancreaticoduodenectomy was performed in six cases, distal pancreatectomy in five cases, excision of cyst in three cases and median segmental pancreatectomy in one case. The mortality rate from surgery was 0%, and the overall perioperative complication rate was 40%. The mean follow-up was 24 months (range from 2 months to 63 months). Two patients, both displaying intraductal papillary mucinous neoplasms, died at 9 and 14 months postoperatively. CONCLUSION: We suggest that cystic neoplasms of the pancreas including intraductal papillary mucinous neoplasm should be resected because they are malignant or pre-malignant. Endoscopic retrograde cholangiopancreatography is the imaging modality of choice for the diagnosis of intraductal papillary mucinous neoplasm. We conclude that the prognosis for resected cystic neoplasms of the pancreas is good.
Cholangiopancreatography, Endoscopic Retrograde ; Cystadenoma, Serous ; Diagnosis ; Female ; Follow-Up Studies ; Humans ; Mortality ; Mucins* ; Pancreas* ; Pancreatectomy ; Pancreaticoduodenectomy ; Prognosis ; Retrospective Studies

In order to explore the existence and distribution of phospholipase (PLC) isozymes in the rat retina, immunohistochemical staining was applied using monoclonal antibodies against PLC isozymes (PLC beta; K92, PLC gamma; D7, F7, PLC delta; R32, S11). For immunohistochemical detection, avidin-biotin peroxidase complex (ABC) method was performed on frozed tissue sections of rat retina. Our study showed that PLC isozymes have particular distributional patterns in the retina. Namely, PLC beta is broadly distributed in the outer and inner segments of photoreceptor cell layer, nuclear layer and ganglion cell layer. PLC gamma is mainly appeared in the nerve fiber layer, ganglion cell layer and inner nuclear layer. PLC delta is confined only in the ganglion cell layer. These results clearly demonstrate the PLC isozymes may have their own role in the transduction of light pathway in the retina. However, further studies will be required to verify theirs precise role in the photoreception.
Animals ; Antibodies, Monoclonal ; Ganglion Cysts ; Immunohistochemistry ; Isoenzymes* ; Nerve Fibers ; Peroxidase ; Phospholipase C beta ; Phospholipases* ; Photoreceptor Cells ; Rats* ; Retina* ; Type C Phospholipases*