This study was carried out to investigate the morphologic findings and process of castration-induced apoptosis in the rat prostate. The experimental group was treated with bilateral orchiectomy followed by sequential sacrifices at 1, 2, 3, 4, 5, 6, 7 days and 2, 3 weeks (6 rats, respectively). Ventral prostate was extirpated and examined by light microscopic and immunohistochemical, ultrastructural observation. Apoptotic index increased by 4 days after castration and decreased thereafter. ApopTag stain revealed brownish granular pattern in the nucleus of apoptotic cells. DNA fragmentation rate was 0.5% in the control group and began to increase by 1 day after castration and reached to 11.1% by 4 days and decreased thereafter. PCNA stain showed brownish granular pattern in the nucleus of some epithelial cells of the prostatic glands. PCNA labelling index was 2.4% in the control group and reached peak by 3 days after castration and decreased thereafter. Electron microscopically, there was chromatin condensation with margination toward the nuclear membrane by 1 day after castration. Also noted were condensation of cytoplasm, dilatation of RER and nuclear fragmentation. Apoptotic bodies were formed and phagocytosed by adjacent cells and some apoptotic bodies were found in the lumen of acini. Based on these results, it can be concluded that castration-induced prostatic involution is the result of apoptosis. Detection of DNA fragmentation with ApopTag is a more a accurate method to identify not only apoptotic body formation itself but also the previous step of apoptotic body formation. PCNA labelling index to identify the cellular proliferation seems to play an active role in the early step of apoptosis and be a good tool for investigation of apoptosis.
Animals ; Apoptosis* ; Castration ; Cell Proliferation ; Chromatin ; Cytoplasm ; Dilatation ; DNA Fragmentation ; Epithelial Cells ; Nuclear Envelope ; Orchiectomy ; Proliferating Cell Nuclear Antigen ; Prostate* ; Rats*

In order to compare the clinical results of Automated Lamellar Keratoplasty with Laser Assisted In Situ Keratomileusis for corection of high myopia, the effects of the procedure on 9 patients(10eyes) following ALK and 14 patients(15 eyes) following LASIK who were followed up at least 6 months were analyzed retrospectively. Mean preoperative spherical equivalent(S.E) was -17.32D(-11.50D~-22.50D) in ALK group and -16.05D(-12.00D~-24.00D) in LASIK group, while mean postoperative S.E was -3.19D at 1 week, -3.50D at 1 month, -4.12D at 3 months, -3.83D at 6 months in ALK group and +2.10D at 1 week, 0.70D at 1 months, -0.10D at 3 months, -0.58D at 6 months in LASIK group. Mean preoperative uncorrected visual acuity was 0.03 in ALK group and 0.04 in LASIK group. At postoperative 6 months, mean uncorrected visual acuity was improved to 0.18 in ALK group and 0.52 in LASIK group. The percentages of patients achieving correction within +/-1.00D at 6 months were 20% in ALK group and 47% in LASIK group. Postoperative cylindrical change was not significant in each group(p>0.05) and also best corrected visual acuity did not show statiscally significant changes in each group(p>0.05). The complications included folds of corneal flap, increased IOP, night halo, and corneal haze. In conclusion, ALK, when performed for high myopia with Ruiz normogram, resulted in undercorrection. LASIK seems to be much more predictable and accurate than ALK in high myopia.
Corneal Transplantation* ; Humans ; Keratomileusis, Laser In Situ ; Myopia* ; Retrospective Studies ; Visual Acuity

No abstract available.
Skin*

The spinal injuries were reported by relatively low incidence than other fracture and dislocation in the body. However, the rate of spine injury tend to increase year by year as the rate of traffic accident and industrial accident have been increased. Authors have experienced 403 cases of various type of injuries from Jan, 1966 to Aug. 1971. They were analysed as follows. 1. Out of 330 patient, males were 212 cases (64.2%) and female 118 (37.8%) Among them 74% of cases were between 20 to 40 years of age. About 51.9% of cases of the spinal injury were caused by traffic road accident. 2. The most most common fracture site in the spinal segments were between 12th thoracic spine and 2nd lumbar spine (69.0%) and most common types of spine injury was simple wedge fracture (70.7%) which is induced by flexion violence. 3. The stable injuries of the spine (69.4% 229 cases) were treated by functional methods such as bed rest, early ambulation. Injured spine were not immobilized in cast. They gave a more functional results than rigidly immobilized group. 4. 27 cases (8.2%) were complicated by paraplegia and mostly (59.3%) were caused by rotational fracture-dislocation. The common site of lesion were between D12-L2 (67.4%) 5. Among the 185 cases who were followed over 6 months, sponetaneous spinal fusion within 6 months after injury occured in 166 cases (89.7%) 76.8% of them were fused within 4 months. 6. Among the 27 paralysed cases, 3 were died within 2 weeks of admission, and 3 cases had complete recovery without residua.
Accidents, Occupational ; Accidents, Traffic ; Bed Rest ; Dislocations ; Early Ambulation ; Female ; Humans ; Incidence ; Male ; Paraplegia ; Spinal Fusion ; Spinal Injuries ; Spine ; Violence

No abstract available.
Neck*

1. Thirty one fractures of the shaft of the tibia were treated by Küntscher intramedullary nailing (closed 26 cases) from Dec. 1979 to Oct. 1985. 2. The fractures were classified into extension type(62%) and flexion type(38%). 3. The straingt nail with bent proximally was used for the extension fractures and the nail with a curve near of each end for the flexion type. 4. In the most cases of simple fracture, we used Küntscher nail of 12mm diameter.interlocking were used in addition to a Küntscher nail(static 13 cases, dynamic 8 cases). In the case of interlocking, nail should be at least 13mm in diameter. 5. The nail was inserted just above or superolateral to the tibial tubercle. 6. The bony union was demonstrated radiographically at 14.4 weeks, and hopitalization period was less than 2 weeks in twelve cases and 3 weeks in the three cases. 7. The major complication were delayed union(1 case) and infection(2 cases).Posterior angulatory deformity greater than 5° appeared in two cases. 8. The cross leg pedicle graft combined with Küntscher nailing would be preferable procedure in the case of open fracture with skin defect.
Congenital Abnormalities ; Fracture Fixation, Intramedullary ; Fractures, Open ; Leg ; Skin ; Tibia ; Transplants

No abstract available.
Temporomandibular Joint*

PURPOSE: The purpose of this study was to compare mammography and breast sonography in detection of breast cancer and to suggest reasonable guideline of breast imaging in breast cancer patients under 30 years of age in whom breast cancer shows different clinicopathologic characteristics compared with breast cancer in older women. MATERIALS AND METHODS: A. uthors reviewed medical records of 27 patients under 30 years of age with pathologically-proven breast cancer. Age, family history, physical examination findings, indications for breast s0nography were reviewed. Cases in whom breast cancer lesion is detectable and cases in whom not detectable using mammography or breast sonography were reviewed. And then, authors evaluated the usefulness of each method and reasons for nonvisualization of lesion on mammography. RESULTS: Among 27 patients, 25 patients had palpable breast mass as indication of mammography and breast sonography. Cancer lesions were detectable in 16 of 25 patients (64%) on mammography and 24 of 25 patients (96%) on breast ultrasonography. Reasons for nonvisualization of cancer lesions on mammography were dense breast with nodular parenchyma pattern and minimal breast change of ductal carcinoma in situ. CONCLUSION: In breast cancer patients under 30 years of age who have palpable breast mass as a initiaJ, and main clinical problem, breast ultrasonography is superior to mammography in detecting and diagnosing breast cancer. We suggest that guidelines can avoid unnecessary mammography in these patients.
Breast Neoplasms* ; Breast* ; Carcinoma, Intraductal, Noninfiltrating ; Female ; Humans ; Mammography ; Medical Records ; Physical Examination ; Ultrasonography* ; Ultrasonography, Mammary

PURPOSE: The purpose of this study was to compare mammography and breast sonography in detection of breast cancer and to suggest reasonable guideline of breast imaging in breast cancer patients under 30 years of age in whom breast cancer shows different clinicopathologic characteristics compared with breast cancer in older women. MATERIALS AND METHODS: A. uthors reviewed medical records of 27 patients under 30 years of age with pathologically-proven breast cancer. Age, family history, physical examination findings, indications for breast s0nography were reviewed. Cases in whom breast cancer lesion is detectable and cases in whom not detectable using mammography or breast sonography were reviewed. And then, authors evaluated the usefulness of each method and reasons for nonvisualization of lesion on mammography. RESULTS: Among 27 patients, 25 patients had palpable breast mass as indication of mammography and breast sonography. Cancer lesions were detectable in 16 of 25 patients (64%) on mammography and 24 of 25 patients (96%) on breast ultrasonography. Reasons for nonvisualization of cancer lesions on mammography were dense breast with nodular parenchyma pattern and minimal breast change of ductal carcinoma in situ. CONCLUSION: In breast cancer patients under 30 years of age who have palpable breast mass as a initiaJ, and main clinical problem, breast ultrasonography is superior to mammography in detecting and diagnosing breast cancer. We suggest that guidelines can avoid unnecessary mammography in these patients.
Breast Neoplasms* ; Breast* ; Carcinoma, Intraductal, Noninfiltrating ; Female ; Humans ; Mammography ; Medical Records ; Physical Examination ; Ultrasonography* ; Ultrasonography, Mammary

Statistical analysis of 18,211 cases of electrocardiogram which were recorded in Chung-Ang University Hospital from January 1969 to December 162% of total cases(61% of male and 63% of female) and the incidence of normal electrocardiogram was decrease with aging. 2) Abnormal Q-wave was seen in 0.4% of total cases(0.5% of male and 0.3% of female) and the incidence of abnormal Q-wave was more common over 50 years of age. 3) Left axis deviation was seen in 1.1% of total cases(1.4% of male and 0.9% of female) and the incidence of left axis deviation was more common over 50 years of age. Right axis deviation was seen in 0.3% of total cases(0.3% of male and 0.4% of female) and the incidence of right axis deviation was more common under 30 years of age. 4) Left ventricular hypertrophy was seen in 3.6% of total cases(4.8% of male and 2.5% of female) and the incidence of right ventricular hypertrophy was more common under 30 years of age. 5) ST-segment depression was seen in 2.0% of total cases(2.2% of male and 1.9% of female) and the incidence of ST-segment depression was increased with aging. 6) T-wave inversion was seen in 1.6% of total cases (1.5% of male and 1.7% of female) and the incidence of T-wave inversion was increased with aging. 7) Atrioventricular block was seen in 1.4% of total cases(1.8% of male and 1.0% of female) and the incidence of atrioventricular block was more common over 50 years of age. 8) Incidence of complete left bundle branch block was 0.2% and was more common overs 60 years of age. Incidence of complete right bundle branch block was 1.1% and the incidence was more common over 50 years of age. Incidence of incomplete right bundle branch block was 3.0% and the incidence was more common under 40 years of age. 9) The incidence of premature beat was 2.7% of total cases, atrial fibrillation 1.5%, supraventricular tachycardia 0.2%, sinus tachycardia 7.5%, sinus bradycardia 2.2% and sinus arrhythmia 2.3%. 10) Low voltage was ssen in 3.8% of total cases and the incidence of low voltage was more common over 50 years of age.
Aging ; Arrhythmia, Sinus ; Atrial Fibrillation ; Atrioventricular Block ; Axis, Cervical Vertebra ; Bradycardia ; Bundle-Branch Block ; Cardiac Complexes, Premature ; Depression ; Electrocardiography* ; Humans ; Hypertrophy, Left Ventricular ; Hypertrophy, Right Ventricular ; Incidence ; Male ; Tachycardia, Sinus ; Tachycardia, Supraventricular