1.Zonulolytic Effects of Alpha-Chymotrypsin and Trypsin.
Nae Youl KANG ; In Sang YOO ; Jae In HAH
Journal of the Korean Ophthalmological Society 1965;6(2):33-35
Zonulolytic effects of alpha-chymotrypsin and trypsin were examined on rabbits' eyes using an apparatus which enables one to press the enucleated eyeballs. The time interval between the applications of enzymes and the protrusion of the lens was measured. The results showed that the trypsin was 4 to 5 times more effective than alpha-chymotrypsin.
Trypsin*
2.Expression of p53 and Rb Proteins in Invasive Ductal Carcinoma of the Breast.
Hyun Jin SON ; Han Sang YOON ; Myoung Jae KANG
Korean Journal of Pathology 1999;33(6):443-449
Inactivation of tumor suppressor genes may play an important role in many human cancers including breast. This study was done to determine the relationship between the expression of p53 and Rb protein and prognostic factors such as histopathologic differentiation, tumor size, and lymph node metastasis. In 57 cases of breast invasive ductal carcinomas, the immunohistochemical staining with p53 and Rb protein gave the following results: p53 protein was detected in 45.6% (26/57) of cases. Tumors with large size, poor differentiation or lymph node metastases tended to show increased expression of p53 protein. However, p53 protein expression did not show any significant correlation with prognostic factors such as tumor size (p value 0.25), histologic grade (p value 0.75), and positive lymph node status (p value 0.26). Rb protein was detected in 57.9% (33/57) of cases. Rb protein also did not show any significant correlation with prognostic factors such as tumor size (p value 0.56), histologic grade (p value 0.71), and positive lymph node status (p value 0.98). There was no significant correlation between p53 expression and Rb protein expression (p value 0.80).
Breast*
;
Carcinoma, Ductal*
;
Genes, Tumor Suppressor
;
Humans
;
Lymph Nodes
;
Neoplasm Metastasis
;
Retinoblastoma Protein*
3.An Experimental Study on Changes of Articular Cartilage following Injury of Ligaments and or Meniscus of the Knee Joint in Adult Rabbits
Sang Won PARK ; Jeong Wha CHU ; Jae Soo KANG
The Journal of the Korean Orthopaedic Association 1982;17(4):567-574
Osteoarthritic changes in experimental animals has been induced by several different methods. In order to study the changes of the articular cartilage induced by injuries of ligaments and/or meniscus of the knee joints, the author performed the experimental study that section of the medial collateral and anterior cruciate ligaments (group I) and section of the medial collateral and anterior cruciate ligaments combined with excision of the medial meniscus (group II) in twenty adult rabbit's knees caused instability. Histological changes of the articular cartilage were observed up to four months. The results were as follows: 1. The changes of the articular cartilage were gradually advanced depend on the time lapse after experimental procedures, in group I and ll. 2. The changes of the articular cartilage could be noticed from eighth week after experimental procedures in group I and from fourth week in group II, and more severe at sixteenth in group I and at twelfth week in group II. 3. The changes of the articular cartilage in group ll were appeared earlier and more severe than in group l. 4. The changes of the articular cartilage in the medial side of the knee joint were noticed more severe than in the lateral side.
Adult
;
Animals
;
Anterior Cruciate Ligament
;
Cartilage, Articular
;
Humans
;
Knee Joint
;
Knee
;
Ligaments
;
Menisci, Tibial
;
Rabbits
4.A Malignant Lymphoma of the Ilium: One Case Report
Sang Won PARK ; Hong Kun LEE ; Jae Soo KANG
The Journal of the Korean Orthopaedic Association 1983;18(3):574-576
Malignant lymphoma of the bone is a rare, but involves the shaft or metaphysis of the bone, producing destruction, frequently pelvis, spine, skull and femur. Most of the patients are over thirty years old and equal sex distribution. The authors experienced one case of lymphoma involving the left ilium.
Femur
;
Humans
;
Ilium
;
Lymphoma
;
Pelvis
;
Sex Distribution
;
Skull
;
Spine
5.A Case of Inherited Thymic Dysplasia Associated with Disseminated Cytomegalovirus Infection.
Seung Yeon NAM ; Mee Ae KANG ; Kang Mo AHN ; Young Jae KOH ; Sang Il LEE
Pediatric Allergy and Respiratory Disease 2000;10(2):171-176
No abstract available.
Cytomegalovirus Infections*
;
Cytomegalovirus*
6.Influence of Type of Screw on the Bacterial Adherence and Infection.
Eun Woo LEE ; Ki Ser KANG ; Soo Yong KANG ; Whui Jae JIN ; Sang In JEONG
The Journal of the Korean Orthopaedic Association 1997;32(1):218-222
The purpose of the study was to evaluate the effect of commonly used orthopaedic material, stainless steel (SS) screw (2.7xlOmm, Synthes) and titanium (Ti) screw (2.7x10mm, Synthes), on bacterial adherence and infection. The bacteria used for this study was Staphylococcus epidermidis for its ability of adherence. For in vitro assay, screws of each material were placed into test tube containing 107 colony forming unit (CFU) of S. epidermidis in trypticase soy broth for 18 hours. Following incubation, each screws were treated either with trypsin or with an ultrasonic cleaner. For in vivo assay, we inoculated 5x107 CFU of S. epidermidis into the proximal tibial metaphysis of the rabbits, and then inserted Ti screw on the right side and SS screw on the left side. The amount of in vitro adherence of S. epidermidis was greater for SS screw than for Ti screw. Especially following trypsin treatment, SS groups yielded significantly higher bacterial counts than Ti group, respectively (1.4x105 CFU vs 2.8x104 CFU, p<0.05). We found a significantly higher bacterial count after use of SS screw than after Ti screw in rabbits in vivo assay also. Trypsin treatment for identifying biofilm embedded bacteria from implanted metal may be useful in the diagnosis of infection. Ti screw appears to have biocompatibility to inhibit bacterial adherence compared with SS screw in the rabbit tibial model.
Bacteria
;
Bacterial Load
;
Biofilms
;
Diagnosis
;
Rabbits
;
Stainless Steel
;
Staphylococcus epidermidis
;
Stem Cells
;
Titanium
;
Trypsin
;
Ultrasonics
7.Traumatic Complete Dislocation of the Knee Joint: Report of 3 Cases
Koon Soon KANG ; Jae In AHN ; Hak Yoon OH ; Young Soo KANG ; Sang Ik HAN
The Journal of the Korean Orthopaedic Association 1983;18(4):777-782
No abstract available in English.
Dislocations
;
Knee Joint
;
Knee
8.A Case of Typhoid Fever Complicated with Empyema of Gall Bladder.
Sang Ho CHOI ; Jae You CHOI ; Byung Hak LIM ; Im Ju KANG ; Sang Hyup KIM
Journal of the Korean Pediatric Society 1988;31(3):386-390
No abstract available.
Empyema*
;
Typhoid Fever*
;
Urinary Bladder*
9.A study on the clinical manifestation and treatment of ectopic pregnancy.
Man Jae LEE ; Sang Kee PARK ; Woo Kang CHUNG ; Sang No YOO ; Ji Hak JUNG
Korean Journal of Obstetrics and Gynecology 1993;36(7):1514-1526
No abstract available.
Female
;
Pregnancy
;
Pregnancy, Ectopic*
10.Development of a novel antigen capture-ELISA using IgY against porcine interleukin-6 and its application.
Deog Yong LEE ; Young Wook CHO ; Sang Gyun KANG ; Sung Jae SHIN ; Han Sang YOO
Journal of Veterinary Science 2004;5(4):337-343
Interleukin-6 (IL-6) is introduced as a marker of disease. At present, a variety of method may be used to quantify expression of this protein. Antigen capture-ELISA is a sensitive and accurate quantification method previously used with ovine, rat, and human IL-6 proteins. However, it has never been reported to quantify porcine IL-6 protein using capture ELISA. In this study, we generated and characterized a set of IgY and mono-specific polyclonal antibodies to recombinant porcine IL-6 (rpIL-6), and combining these with a sensitive and specific capture-ELISA for a diagnostic purpose. cDNA encoding the mature protein coding region of porcine IL-6 was cloned and expressed with pQE-30UA expression vector. rpIL-6 was then expressed and purified by using Ni-NTA resin. Protein mass of 24 kDa was found with SDS-PAGE and the identity of the protein was confirmed by Western-blot. Production of polyclonal antibodies against rpIL-6 was performed using the purified rpIL-6 in mice and hens. An antigen capture-ELISA was developed with the antibodies after their extraction. To compare the IL-6 level in the different sanitary state of farms, pig sera were randomly collected and concentration of IL-6 in the sera was measured with the antigen capture-ELISA. The capture-ELISA with the optimal concentration of antibodies, in this study, was able to detect about 10 ng/ml of rpIL-6. IL-6 levels determined with the capture-ELISA in pig sera showed positive correlation with the sanitary states of the farms. These results suggested that the developed antigen capture-ELISA could be a good tool for the screening of microbial infection in pig farms.
Animals
;
Biological Markers/blood
;
Blotting, Western/veterinary
;
Chickens
;
Cloning, Molecular
;
DNA, Complementary/genetics/isolation&purification
;
Electrophoresis, Polyacrylamide Gel/veterinary
;
Enzyme-Linked Immunosorbent Assay/methods/*veterinary
;
Female
;
Immunoglobulins/*blood
;
Interleukin-6/*immunology
;
Mice
;
Mice, Inbred ICR
;
Recombinant Proteins/immunology
;
Swine/*immunology