Author investigated the deposition of immunoglobulins and complements in thc skin of 56 patients with 19 various dermatoses by direct immunofluorescent (DIF) staining. The biopsied specimens were quick-frozen(by dry ice-acetone) and stained with FITC conjugated antihuman immunoglobulin and complement after cutting in a cryostat at -20C~30C. (countinued...)
Complement System Proteins ; Dronabinol ; Fluorescein-5-isothiocyanate ; Humans ; Immunoglobulins ; Skin ; Skin Diseases*

No abstract available.
Migraine Disorders*

In order to isolate the keratinophilic fungi from the soil, 7x10cm vinyl envelope were used in shich 10 rhomboid shaped holes (1x1cm) were made, so that when the human scalp hairs as baiting material were laid out straight in the envelope the keratinophilic fungi would be baited to the open part of the hairs in envilope in the 6 different places for 20 days in Kyungpood National University Hospital campus from April 1967 to September 1967. The author's newly improved baiting method with the hairs in the vinyl envelope was simple and the results were more satisfactory for isolating keratinophilic fungi compared with the original Vanvreuseghem's hair baiting method.
Fungi* ; Hair ; Humans ; Scalp ; Soil

No abstract available.
Diagnosis* ; Hypertension*

In the diagnosis of parasitic helminthic diseases, the value of examining and identifying the parasitic eggs and/or adult parasites from patient's urine or stool are well appreciated. However, these methods have a limited value in the diagnosis of tissue or intracellular parasitism, and we have to rely on supplementary methods such as immuno-serological test. The author tested the value of gel precipitation reactions as a diagnostic method of clonorchiasis by observing the appearance of bads on rats experimentally infected with Clonorchis sinensis. And the therapeutic effect of CIBA 35'058-Ba was evaluated by this serological method. The antigen was prepared from the adult worms infected in rabbits by Tsuji method. Rats infected with 40 metacercariae each were bled on 7, 14, 21, 26, 28, 39, 42(43), 49(53) days after infection to observe the appearance of precipitin bands by both Pucterlony method and immunoelectrophoresis. Fifteen rats were separately infected and treated with CIBA 35'058-Ba in dose of 15mg/kg of body weight. The following results were obtained : 1. It was observed that there exist individual variations in the appearance of the first precipitin band with the range of 2-4 weeks after infection. 2. The number of precipitin bands was increased until 6-7 weeks after infection. In all cases, 3 precipitin bands were appeared by Oucterlony method and 6-7 bands were appeared by immunoelectrophoresis after 6-7 weeks of infection. 3. It was hardly possible to notice any change in the number of bands after the administration of CIBA 35'058-Ba. This result suggested that the drug has no effect on clonorchiasis which was confirmed by the autopsy of the experimental rats.
Adult ; Animals ; Autopsy ; Body Weight ; Clonorchiasis ; Clonorchis sinensis* ; Diagnosis ; Eggs ; Helminths ; Humans ; Immunoelectrophoresis ; Metacercariae ; Ovum ; Parasites ; Rabbits ; Rats*

No abstract available.

No abstract available.
Anxiety Disorders* ; Anxiety* ; Humans ; Lymphocyte Subsets* ; Lymphocytes*

Fibronectin(FN) is a major component of the extracellular matrix and is able to bind to cells and other components of the matrix. Although the cell producing the largest amounts of fibronectin is fibroblast, the pro duct,ion on FN also has been described in cultured keratinocyt,e and epithelial tumor cells of basal cell carcinoma(BCC). Recently, functional role nf FN in relation to biologic behavior of BCC and squamous cell carcinoma(SCC) is open to speculation. The authors investigated the localization of FN in the lesional skin of 5 cases of BCC, 4 of SCC, 5 of psoriasis and 5 normal skin using direct immunofluorescence technique with antifibronectin antibody to find out the production of FN in keratinocytes and tumor cells. 1. In the skin of BCC, FN was presented in a thick, linear depositions along the margin of tumor lobules in all cases, and as fillamentous deposits or scattered points in the nest of tumor cells in 3 cases. 2. In the skin of SCC, FN was presented in a thin, coarse depositions around the margin of tumor but not presented within the tumor nest in all cases. R. In psoriasis, FN was observed in horney layer and upper part of epidermis. It was presented more abundantly in dermo-epidermal junction, papillary and reticular dermis than in normal skin. 4. In normal skin, FN was absent in the epidermis but presented in dermo-epidermal junction and blood vessel wall of upper dermis, the amount of FN was decreased from papillary to lower dermis.
Blood Vessels ; Carcinoma, Basal Cell* ; Carcinoma, Squamous Cell ; Dermis ; Epidermis ; Extracellular Matrix ; Fibroblasts ; Fibronectins* ; Fluorescent Antibody Technique, Direct ; Keratinocytes ; Psoriasis* ; Skin*

Clofazimine (Lamprene or B668) is a phenazine congener that is used in leprosy, and the patients treated with the drug may develop red discoloration, dark brown pigmentation and ichthyotic skin. The authors observed 181 leprosy patients who had developecl discoloration, pigmentation and ichthyotic skin during clofazimine therapy, as well as disappearance of the pigmentation after stop of it. The results are summarized as follows: 1) Reddish discoloration was most frequently observed 2 weeks after beginning treatment in 66 patients (50. 4%), which showed earlier in the higher dosage grow up, 2) Dark brown pigmentation was iviost frequently observed 4 weeks after beginning treatment in 64 patients (48. 9%), which showed earlier in the higher dosage group. R) For histopathological evaluation of pigmentation, various ataining rnethods were used. On unstained frozen sections, yellow brown crystals were scattered in the dermis of both discolored and pigmented skin, and in H R E stain pigments, melanin were niarkedly increased in the epidermal basal layer and a, faint yellow brown. ghost was seen in dermis of pigmented skin. With fat stains using oil-red-0 and Sudan III, reddish amorphous materials were scattered in and around the cytoplasm of the macrophages c>f the pigmented skin. 4) Ichthyotic skin was observed in 97 patients, and most frequently 2 and R months after beginning treatment in 26 patients (26. 8%), but it was never observec1 in 34 of all patients in spite of a continuous intake of the drug for more than a. year. -countinue-
Clofazimine* ; Coloring Agents ; Cytoplasm ; Dermis ; Frozen Sections ; Humans ; Leprosy* ; Macrophages ; Melanins ; Pigmentation ; Skin ; Sudan

No abstract available.
Tendons*