The RNA genome of poliovirus encodes a long polyprotein precursor and this polyprotein is cleaved proteolytically by viral protease to yield mature proteins. The mature proteins derived from the Pl polyprotein precursor are the component of capsids. To further delineate the process of capsid assembly and encapsidation, in a first attempt, a cell line which expresses the authentic Pl polyprotein was established. CV-1 cells were transfected with the pRCRSVS1P1 plasmid DNA which contains 5'ncr sequences, whole authentic capsid gene of poliovirus and neomycin resistance gene. These cells were treated with G418 for 3 months, and eventually G418 resistant cells were selected and formed colonies. Each colony was picked and grown in the media containing G418. DNA analysis indicated that 1 of 13 neomycin resistant cell lines (R2-18) contains whole poliovirus Pl capsid gene segment which was incorporated into the genome. Immuneprecipitation of cell lysates with sera from rabbit immunized with inactivateded Sabin type 1 particles demonstrated the constitutive expression of the poliovirus Pl capsid protein from R2-18.
Capsid Proteins* ; Capsid* ; Cell Line* ; DNA ; Genome ; Haplorhini* ; Kidney* ; Neomycin ; Plasmids ; Poliovirus* ; RNA

No abstract available.
Endoderm* ; Endodermal Sinus Tumor*

No abstract available.
Disability Evaluation*

No abstract available.
Bleomycin* ; Cisplatin* ; Drug Therapy, Combination* ; Etoposide* ; Liver* ; Lung* ; Wilms Tumor*

Serum immunoglobulins from carp Cyprinus carpio were purified using affinity chromatography methods. Fish were immunized with bovine serum albumin (BSA) and the specific fish antibodies purified from the immune serum on a BSA-irnmobilized Sepharose 4B gel. The analysis of the immunoglobulins by reducing SDS-PAGE showed them to be composed of a single p,-like heavy chain of 76 kd and light chain of 28 kd. Polyclonal rabbit antibodies against the fish IgM were produced to further analyze IgM' B-like cells from carp. Irrespective of a BSA immunization, the distribution rates of IgM' B-like cells in the head kidney and spleen were about 49% and 24%, respectively. The IgM' cells were magnetically purified by using Mini-Macs column. To study whether the purified IgM' cells are B-like lymphocytes, those cells were cultured with hrIL-4 (50 U/ml) for 48 hr at 25C in 5% CO, incubator. And the titer of antibodies secreted from IgM' and IgM cells was analyzed by an enzyme-linked immunosorbent assay (ELISA). We found the IgM' cells produced a greater amount of antibodies to BSA than both IgM cells and negative control. Unexpectedly, however, moderate amount of antibodies were also detected in the supernatant of IgM cell population, indicating the difference of humoral immune responses between a fish and mammalian.
Antibodies ; Carps ; Chromatography, Affinity ; Electrophoresis, Polyacrylamide Gel ; Enzyme-Linked Immunosorbent Assay ; Head Kidney ; Immunity, Humoral ; Immunization ; Immunoglobulin M ; Immunoglobulins ; Incubators ; Lymphocytes ; Sepharose ; Serum Albumin, Bovine ; Spleen

PURPOSE: To find the magnetic resonance (MR) imaging patterns and to determine the viability in normal, infracted and reversible ischemic testis of the rat. MATERIALS AND METHODS: Fifteen Sprague-Dawley rats were examined and they were divided into four groups. Group 1 was the control group, group 2 had a complete testicular artery ligation, group 3 had a complete ligation with reperfusion after 1 hour and group 4 had a complete ligation with reperfusion after 12 hours. All four groups were imaged every 5 minutes for 30 minutes. Delayed MR imaging was obtained every 30 minutes for 90 minutes. Two follow-up MR images were performed in all groups at a one-week interval. The signal intensity was measured in the normal testis, ischemic testis, and in muscle, water and fat in every rat at each time, with the phantom attached near the scrotum during the scanning. The signal intensities were analyzed statistically. RESULTS: On initial and 2-week follow-up examinations, the pattern of change differed among four groups (p<0.001). Group 1 and Group 3 did not show any marked change over time at each examination. Group 3 showed strong enhancement at the first week follow-up. Group 2 showed steadily delayed enhancement at each examination. Group 4 had same pattern with the Group 2 with higher enhancement intensity in parallel. CONCLUSION: MR images with Gd-DTPA could be useful for the diagnosis of damaged testicular tissue and for the determination of testicular viability.
Animals ; Arteries ; Diagnosis ; Follow-Up Studies ; Gadolinium DTPA* ; Ischemia* ; Ligation ; Magnetic Resonance Imaging* ; Models, Animal* ; Rats* ; Rats, Sprague-Dawley ; Reperfusion ; Scrotum ; Testis ; Water

No abstract available.
Ifosfamide*

No abstract available.

Two hundreds and ninty-four prostitutes of foreign soldiers of K2 area in Taegu were investigated for their general circumtances, clinical and bacteriological studys from Mar. 1978 to May 1979. The results were as foIIows: The age group of 20-24 year-old constituted highest proportion at the level of about 53.4% Averge age of total casea was about 26.2. The propartion of those who were more than 35 occupied about 7.2%. The positive rate of Cram negative displococci (GND) on dirert smers showed highest in December and, January and lowest in-April. Wcekly distribution shwed a higher in second and fourth week. Nongonococcal infect.ions occupied about 45.4%. and total raes. The causative oanism. were niostly Gram negative and Gram positive bacilli. The mothly spells and perons with gonorrhea priod prevaIence rate showed a higher rate in Becember, January and July. The prevalence ratio of gonorrhea and syphilis was 13: 1. The examined cases occupied about 34.3, during first 2 month is after registration. The seasoal vari tion of registrators and cancellators were both most in winter. The positive rate of GND was ahout 53.1% in new comers less than two month-examinations after regiatration. Cervical erosion or chronic cervicitis .;howed about 44.8% among the examined 96% cases. Gonococci were isoIated about 25.6% among the ones with cervical lesions on Thayer-Martin media. On the other hand, the ones without lesions were about 11.3% comparatively. Syphilis period prevalence rate was about 8. 5 A. The titers of VDRL slide test were low (1; 8 or less). Two-thirds of positive cases of GND were treated by penicillin, although tetracycline and spectinomycin were also used in a few cases. The penicillin hypersensitivity sho- wed positive results in 9.7% of all treated cases.
Daegu ; Gonorrhea ; Hand ; Humans ; Hypersensitivity ; Military Personnel* ; Penicillins ; Prevalence ; Sex Workers* ; Sexually Transmitted Diseases* ; Spectinomycin ; Syphilis ; Tetracycline ; Uterine Cervicitis

A case of 38 year old woman had noted 3 lesions with asymptomatic, reddish brown colored, coin sized, slightly elevated, confluent patches on the right postauri-cular for about 20 years. The infiltrate in the patches felt moderately firm to the touch. the lesions were suspected to be chronic dermatitis group, erythema multiforme or granuloma annulare clinically, but the histopathologic features showed the typical findings of granuloma faciale. Literature was briefly reviewed for the discussion and comparision of clinical features and histopathological findings of author's case with the 2 cases previously reported in Korea.
Adult ; Dermatitis ; Erythema Multiforme ; Female ; Granuloma Annulare ; Granuloma* ; Humans ; Korea ; Numismatics