BACKGROUND: The action of ultraviolet rays on DNA causes the main photobiologic response of cells to ultraviolet rays. To study this effect, tritiated thymidine autoradiography was used. Recently 5-bromo-2deoxyuridine(BrdU), an analogue of thymidine, immunohistochemistry has been developed and is used in the detection of synthetic phase cells. Compared to autoradiography, there are several advantages of BrdU immunohistochemistry; a shorter processing time, no requirement of specific facilites. PUVA, the combination method of UVA and Psoralen has lots of photobiologic effects. OBJECTIVE: Using Brdu immunohistochemistry, the effect of PUVA on the DNA synthesis of tape stripped mouse epdermis was studied. METHOD: Mice stripped by adhesive tape for enhancing DNA synthesis were injected intraperitoneally with 50mg/kg of BrdU immediately after stripping and at 6, 12, 14, 16, 18, 20, 22, 24 and 48 hours after tape stripping for decision of the time for PUVA. The skin diopsies were taken and the specimens were stained by BrdU immunohistochemistry. Single systemic PUVA exposure was performed on the stripped epidermis in peak synthetic time after tape stripping. The irradiation dose of UVA was 5J/cm(2). 8-MOP was administered at 90 minutes before UVA irradiation via a feeding tube with the dose of 16mg/kg. Mice were injected intraperitoneally with 50mg/kg of BrdU immediately after PUVA and at 12, 24, 48, 72 hours, and 7 days after PUVA. The skin biopsies were taken and the specimens were stained by BrdU immunohistochemistry. Positively labeled cells were counted per 5mm epidermis. RESULT: The results can be summerized as follows : 1. The mean numbers of BrdU labeled cells of each groups according to time after tape stripping were 11.0+/-4.4 at immediate, 24.0+/-9.7 at 6 hours 31.4+/-18.1 at 12 hours, 55.0+/-16.1 at 14 hors, 25.8+/-9.7 at 16 hors, 44.2+/-15.7 at 18 hors, 47.6+/-15.6 at 20 hors, 33.4+/-12.3 at 22 hors, 38.0+/-16.3 at 24 hors, and 22.0+/-8.2 at 48 hors group. The mean number of BrdU labeled cells was observed at 14 hors after tape stripping (p<0.05). So by tape stripping DNA synthesis was enhanced maximally at 14 hours after tape stripping. 2. The man numbers of BrdU labeled cells of each groups according to time after PUVA were 11.0+/-7.5 at immediate, 32.2+/-13.2 at immediate, 32.2+/-13.2 at 6hors, 26.4+/-13.4 at 24 hours, 18.0+/-3.4 at 48 hours, 40.3+/-8.3 at 72 hours, and 27.8+/-11.0 at 7 days group. The lowest number of BrdU labeled cells was observed immediately after PUVA(p<0.05). The decreasein the number of BrdU labeled cells significantly persisted 48 hours after PUVA(p<0.05). CONCLUSION: The inhibitory effect on DNA synthesis of PUVA might be sustained 48 hours after PUVA. DNA synthesis was recovered at 72 hours after PUVA and sustained for 7 days.
Adhesives ; Animals ; Autoradiography ; Biopsy ; Bromodeoxyuridine ; DNA* ; Epidermis ; Ficusin ; Immunohistochemistry* ; Methoxsalen ; Mice ; Skin ; Thymidine ; Ultraviolet Rays

index (%) in unirradiated mouse skin was 11.0+/-4.3. LI was significantly increased by tape stripping to 22.1+/-4.6. 2. The number of SBC in 1cm epidermis after 50mJ/cm UVB exposure was 28.2+/-4.1. The number of SBC was increased by tape stripping to 57.4+Cell proliferation, by evaluating sunburn cell (SBC) formation, was studied in mouse skin following tape stripping and ultraviolet light B (UVB) exposun.. 1-radiation was achieved using high pressure mercury are UVB. The results are summarized as follows. 1. Labeling 19.2. These results suggest that proliferating cells are more sensitive to UVB exposure.
Animals ; Cell Proliferation* ; Epidermis ; Mice ; Skin ; Sunburn* ; Ultraviolet Rays

We report a case of anosacral cutaneous amyloidosis in a 74 year-old-man. The skin finding is a patch of the size of a childs palm on the sacral area, consisting of brownish macular lines that radiate frorn the anus. Histopathology revealed defiosits of homogenous eosinophilic mateial s in the papillary dermis. Congo red staining and dylon staining was positive. Immuohistochemical staining using anti SAP antibodies and anti-keratin antibodies showed positive reactions.
Amyloidosis* ; Anal Canal ; Antibodies ; Child ; Congo Red ; Dermis ; Eosinophils ; Humans ; Skin

BACKGROUND: Pruritus, which is a frequent accompanying symptom of dermatological diseases, is also common during pregnancy, either localized or generalized. It may be related to specific dermatoses of pregnancy. OBJECTIVE: A clinical study was performed to know the exact nature of pruritus in pregnancy. METHODS: Subjects with systemic diseases, or laboratory abnormalities were excluded. People who have had pruritic dermatologic diseases before pregnancy were also excluded. One hundred and fifty five pregnant women were interviewed with physical examinations and followed up to the date of delivery. Pruritus was graded. RESULTS: Pruritus was present in 31.6%. Most affected subjects had mild or moderate severity. The abdomen was the most common site. There was a tendency for the duration of itching to increase with the duration of pregnancy. CONCLUSION: Conclusion: These findings may provide basic and useful data on pruritus during pregnancy.
Abdomen ; Clinical Study* ; Female ; Humans ; Physical Examination ; Pregnancy* ; Pregnant Women ; Pruritus* ; Skin Diseases

No abstract available.
Blood Pressure* ; Child* ; Humans ; Seoul*

Inflammatory polyposis of the colon is rare portion of the colon polyp entity. We experienced three patieats who suffered from colitis of unknown'etiology and discovered membranous retkular with/ without filiform polyp in the distal sigmoid colon of the them. Case 1, 56-year-old female, combined with basaloid cercinoma of the rectal canal that confirmed by pathology after abdominoperineal resection. So we obtained specimen of inflammtory polyp end tried to evaluate the mechanism of it. We found healed ulcer under the mucosal bridge. Case 2. 55-year-old male, had been suffered from irritable bowel syndrome. Prior to visit outpatient clinic, he complained tenesmus with diarrhea for a week. Inflammatory polyp was discovered by colonoacopy but his symptoms relieved by tranqulizers only. Case 3. 57-year-old male, has been a good health. He undertook colonoscopy for routine check thst revealed membranous reticular with filiform inflammatory polyp from distal sigmoid colon to hepatic flexure. Such shape of inflammatory polyp was not documented in Korea, so we reported three cases of it with review of the literature.
Ambulatory Care Facilities ; Colitis ; Colon* ; Colon, Sigmoid ; Colonoscopy ; Diarrhea ; Female ; Humans ; Irritable Bowel Syndrome ; Korea ; Male ; Middle Aged ; Pathology ; Polyps* ; Ulcer

After UV irradiation to mouse epidermis, autoradiography using H-thymidine was performed to study the effects of UV on scheduled and unschcdu ed DNA syntheses. The results can be summarized as follows. l. In case of UVB 100mJ/cm, heavily labeled cells (HLCs) representing scheduled DNA synthesis began to decrease immediately after UVB irradiai,ior and were significantly decreased after 24 hours, but then recovered after 48 hours. The ecovery was maintained from 72 hours, up to 7 days. Sparsely labeled cells (SLCs) repres inting unscheduled DNA synthesis were significantly increased from immediately after UV 3 irradiation and up to 6 hours later. Repair was present at 24 hours, and was maintained at 48 hours, 72 hours, and 7 day after irradiation. 2. In case of UVA, UVA 10J/cm did not affect DNA synthesis sign ficantly. But in cases of UVA 30J/cm and 50J/cm, HLCs began to decrease immediately after UVA irradiation and were significantly decreased for 24 hours. Recovery occured at 48 hours, and was maintained from 72 hours up to 7 days. SLCs were significently increased immediately after and up to 6 hours after UVB irradiation. Repair generally occurred after 24 hours, and was maintained at 48 hours, 72 hours, and 7 days after ir rad ation.
Animals ; Autoradiography ; DNA* ; Epidermis ; Mice*

BACKGROUND: Lichen planus is a papulosquarnous disease characterized by pruritic violaceous papules most commonly an the extremities of middle-aged adults. It may or may not be accompanied by oral and genital mucous membrane involvement. Its course is generally self-limited for a period of several months to years, but it may last indefinitely. OBJECTIVE: This study was performed to find the clinical characteristics of lichen planus. METHODS: Thirty-five cases of lichen planus comfirrned clinically and histopathologically were analyzed. RESULTS: The incidenc of lichen planus was 0.073% of the total number of dermatologic patients. The age distribution was in the range of 14-63 years old being most prevalent in the 6th decade and there was ariequal sex ratio. The most common site of involvement was the mucous membrane, and the extremities were more common than the trunk. Pruitus or pain and discomfort developed in 28 pat,ients(80% ). In most cases, the treatment was topical corticosteroid and antihistamine. Sixteen cses improved within 1 month to 2 years. Five cases recurred in the improved cases. CONCLUSION: The clinical analysis of 35 patients with lichen planus was performed. The incidence of lichen planus was 0.073% of the total number of dermatologic patients. Males and females were equally affected Mucous membrane involvement was detected in 21 patients(60%).
Adult ; Age Distribution ; Extremities ; Female ; Humans ; Incidence ; Lichen Planus* ; Lichens* ; Male ; Mucous Membrane ; Sex Ratio

BACKGROUND: While the effect of the flashlamp pumped dye laser in the treatment of telangiectasia and other vascular disease has been reported by many authors, there have been a few reports on the therapeutic effect of the copper vapor laser(CVL) on telangiectasia and other vascular lesions. OBJECTIVE: Our purpose was to assess the clinical results in Korean patients who had cutaneous vascular disorders that were treated with the CVL at 578 nm. METHODS: A total of 23 patients with vascular lesions such as telangiectasia were involved. The effect of the laser treatment was evaluated 3 months after the last treatment. RESULTS: Of the 15 patients with telangiectasia, 12 patients showed good or excellent responses. 2 out of 3 patients with strawberry hemangioma showed good results. As for the other lesions, 2 out of the remaining patients 5 showed excellent results. CONCLUSION: The CVL has a beneficial effect and is a good alternative treatment modality for vascular lesions.
Copper* ; Hemangioma, Cavernous ; Humans ; Lasers, Dye ; Lasers, Gas* ; Telangiectasis ; Vascular Diseases

BACKGROUND: Chronic cutaneous lupus erythematosus(CCLE) is a well-known disease entity. But there has been no data about its clinical behavior and histopathologic features in Korea. OBJECTIVES: This study was conducted to elucidate the clinical, laboratory, and histopathologic features of CCLE, and the relationship between CCLE and SLE. MATERIALS AND METHODS: We investigated 48 cases of CCLE that visited the department of dermatology at the Seoul National University Hospital from January 1990 to June 1997. Medical records and biopsy slides were reviewed.
Biopsy ; Dermatology ; Korea ; Lupus Erythematosus, Cutaneous* ; Medical Records ; Seoul