The adult trematode, Fasciola hepatica and Eurytrema pancreaticum, employed in this experiment were obtained from the cattle slaughtered at the local abbatoir. The worms were selected and washed several times in normal sterilized saline solution. Each ten of intact F. hepatica and about thirty to fifty of E. pancreaticum were incubated in 50 cc volume of special incubation flasks with incubation medium consisting of 50 cc of Krebs-Ringer phosohate buffer (pH 7.4). The incubation medium was added C(14)-lactate and non-radioactive carrier Na-lactate so as to contain lactate concentration of 32 mg per cent. The worms were allowed to incubate for 3 hours in the Dubnoff metabolic shaking incubator at 38 C. After incubation period, respiratory CO(2) samples from central wall of incubation flask were analysed for total CO(2) production rate and their specific activity of respiratory CO(2). The lactate uptake rate was determined by analyzing the the difference between lactate concentration in a medium before and after the incubation period, and the pyruvate appearance rate was dertermined by analyzing the pyruvate concentration in a medium after incubation. The glycogen samples isolated from worms were analyzed for the tissue concentration and their radioactivities in order to determine the turnover rate of glycogen pool. Radioactivities of these serise of experiment were counted by an endwindow Geiger-Muller counter as an infinitely thin samples. The quantative analysis of C(14)-lactate utilized by F. hepatica and E. pancreaticum were summerized and compared as following. In F. hepatica the lactate uptake rate was a mean value of 1.04+/-0.15 micromole/hr/g of wet wt. and pyruvate apperance rate was a mean value of 0.132+/-0.005 micro-mole/hr/g of wet wt. The total CO(2) production rate by the flukes averaged 13.82+/-0.75 micro-mole/hr/g of wet wt. The relative specific activities of respiratory CO(2) showed a mean value of 9.93+/-0.62 per cent. The rate of CO(2) production derived from medium C(14)-lactate was a mean of 1.38+/-0.13 micro-mole/hr/g of wet wt. Therefore the averge value of 55.27+/-5.78 per cent (R.L.D. CO(2)) and 15.35+/-1.90 per cent (R.L.D. pyr) of lactate was oxidized into respiratory CO(2) and pyruvate respectively. On the other hand, in E. pancreaticum the lactate uptake rate was a mean value of 0.61+/-0.18 micromole/hr/g of wet wt, and pyruvate appearance rate was a mean of 0.023+/-0.001 micromole/hr/g of wet wt. The total CO(2) production rate by the E. pancreaticum averaged 4.29+/-0.85 micromole/hr/g of wet wt. The relative specific activity of respiratory CO(2) (R.S.A CO2) showed a mean value of 9.20+/-0.34 per cent. Thus, a mean value of 9.20 per cent of total CO(2) production rates was originated from C14-lactate in a medium, therefore the rate of CO(2) production derived from medium C(14)-lactate was a mean value of 0.40+/-0.10 micromole/hr/g of wet wt. The average value of 23.93+/-7.11 per cent(R.L.D. CO(2)) and 3.86+/-0.45 per cent(R.L.D. pyr) of lactate was oxidized into respiratory CO(2) and pyruvate respectively. The tissue concentration of glycogen in F. hepatica was a mean of 2.63 per cent/g of wet wt, while in E. pancreaticum was a mean of 4.06 per cent/g of wet wt. The turnover rate of glycogen pool in F. hepatica yielded a value of 0.073+/-0.008 micromole/hr/g of wet wt whereas in E. pancreaticum yielded only a mean of 0.006+/-0.002 mg/hr/g of wet wt. Therefore, the half time of glycogen turnover, which is the time interval required to replace the half of glycogen pool with medium C(14)-lactate, gave value of a mean of 10.73+_0.76 days in F. hepatica. However, incorporation of C(14)-lactate into glycogen was negligible in the E. pancreaticum. Theses data impressed that the carbohydrate such as lactate may play a role of major part of their oxidative metabolism in F. hepatica, whereas minor part of lactate participates in the oxidative metabolism in E. pancreaticum.
parasitology-helminth-trematoda ; Fasciola hepatica ; Eurytrema pancreaticum ; autoradiography ; biochemistry ; pyruvate ; lactate ; glycogen ; metabolism ; Krebs-Rigner phosphate buffer

No abstract available.
Insurance* ; Korea* ; Societies, Medical*

No abstract available.
Immunoglobulin G*

No abstract available.
Humans ; Mass Screening*

No abstract available.
Prospective Payment System* ; Prospective Studies*

No abstract available.

No abstract available.
Enterocolitis, Necrotizing* ; Soy Milk*

Twelve hyperlipidemic patients taking ginseng extract 1.0 gm daily were studied for sequential changes in blood lipid levels for 16 weeks. Following results were observed. 1. HDL-C was markedly elevated from the beginning through test period while TG is conversely decreased. 2. TC and LDL-C revealed the tendency to decrease but not significant in degree. 3. No remarkable untoward reaction was observed with long term ginseng extract administration. In summary, the beneficial effect of ginseng extract on abnormal blood lipids level is unequivocal and the author recommend the use of ginseng extract to patient being on the verge of atherosclerosis.
Atherosclerosis* ; Humans ; Panax*

In our hospital, we experienced a case of left solitary cystic kidney. She was sixty years old woman. Her chief complaints were dull pain on left upper abdomen, terminal dysuria. tenesmus. and frequency. Cyst was situated to lower pole of kidney. Cyst was dissected from renal parenchyma. It contained about 250cc serous and clear cystic fluid, Post-operative general condition was good and her chief complaints were disappeared almostly.
Abdomen ; Bone Cysts* ; Dysuria ; Female ; Humans ; Kidney*

A carefull inspection of an AP X-ray of a normal hip reveals in the weight-bearing surface of the ilium a curved area of dense bone, called SOURCIL by Pauwels (1963). The configuration of this dense area is semilunar and represents subchnodral bony eburnation due to a respose by the articular portion of the ilium to the stress provoked by the compressive force acting on it. However, in many cases of idiopathic avascular necrosis of the femoral head, changes in SOURCIL may be seen. So we reviewed our 49 patients (unilateral 16, bilateral 33) since 1983 with comparison to normal hips (100 persons). The results are as follows; 1. SOURCIL is not visible at birth and by walking, it is developed with age and has the adult configuration at the age of 18. It is thought the configuration and sloping angle of SOURCIL is not changed with age after skeletal growth is completed. 2. Although the position of the X-ray tube may be changed, the shape and angle of SOURCIL is not changed significantly. 3. The average SOURCIL angle is 2.1° in normal hip group and 3.5° for the avascular necrosis group but, there is no meaningful statistical differences. It seems to be meaningful that the mean SOURCIL angle is 10.3° for patients under the age of 35 in avascular necrosis groups (9 persons). 4. If a high SOURCIL angle (about 10° or more) is present, the hemiarthroplasty such as bipolar endoprosthesis should be avoided because there is biomechanically far more wearing of the acetabular roof after that kind of operation. If inevitable, deepening the acetabulum by reaming is recommended for prevention of superolateral migration of prosthesis after operation. 5. With close observation of SOURCIL before and after operation, one can easily point out what is able to predict the prognosis on the biomechanical basis.
Acetabulum ; Adult ; Head ; Hemiarthroplasty ; Hip ; Humans ; Ilium ; Necrosis ; Parturition ; Prognosis ; Prostheses and Implants ; Walking ; Weight-Bearing