Serum immunoglobulins from carp Cyprinus carpio were purified using affinity chromatography methods. Fish were immunized with bovine serum albumin (BSA) and the specific fish antibodies purified from the immune serum on a BSA-irnmobilized Sepharose 4B gel. The analysis of the immunoglobulins by reducing SDS-PAGE showed them to be composed of a single p,-like heavy chain of 76 kd and light chain of 28 kd. Polyclonal rabbit antibodies against the fish IgM were produced to further analyze IgM' B-like cells from carp. Irrespective of a BSA immunization, the distribution rates of IgM' B-like cells in the head kidney and spleen were about 49% and 24%, respectively. The IgM' cells were magnetically purified by using Mini-Macs column. To study whether the purified IgM' cells are B-like lymphocytes, those cells were cultured with hrIL-4 (50 U/ml) for 48 hr at 25C in 5% CO, incubator. And the titer of antibodies secreted from IgM' and IgM cells was analyzed by an enzyme-linked immunosorbent assay (ELISA). We found the IgM' cells produced a greater amount of antibodies to BSA than both IgM cells and negative control. Unexpectedly, however, moderate amount of antibodies were also detected in the supernatant of IgM cell population, indicating the difference of humoral immune responses between a fish and mammalian.
Antibodies ; Carps ; Chromatography, Affinity ; Electrophoresis, Polyacrylamide Gel ; Enzyme-Linked Immunosorbent Assay ; Head Kidney ; Immunity, Humoral ; Immunization ; Immunoglobulin M ; Immunoglobulins ; Incubators ; Lymphocytes ; Sepharose ; Serum Albumin, Bovine ; Spleen

I deeply regret but request a retraction of our paper published in Immune Network.

I deeply regret but request a retraction of our paper published in Immune Network.

Carp which receive intraperitoneal injections of sodium alginate show a high survival rate after being challenged with Edwardsiella tarda. To elucidate the immunoenhancement by sodium alginate, its effects on the non-specific defense system of carp were investigated. Sodium alginate had little influence either on the activity of the alternative complement pathway or on the phagocytic and respiratory burst activities of head kidney phagocytes (HKP), yet it greatly enhanced the migration of HKP to the peritoneal cavity (the site of injection) and concurrently elevated their phagocytic activity. The number of phagocytes mobilized by sodium alginate was 2 to 50 times greater than that by the well-known peritoneal exudate cell-eliciting agents when injected at the same dose. Accordingly, it is highly probable that the early elimination of challenge bacteria by such mobilized and activated phagocytes was responsible for the high survival rate of the alginateinjected fish. In chemotaxis assays, it was revealed that sodium alginate stimulated sorne leukocyte subpopulation (s) within the peritoneal cavity to produce and/or secrete chemotactic factor (s), while concurrently enhancing the sensitivity of HKP to the factor (s).
Bacteria ; Carps* ; Chemotaxis ; Complement Pathway, Alternative ; Edwardsiella tarda ; Exudates and Transudates ; Head Kidney ; Injections, Intraperitoneal ; Leukocytes ; Peritoneal Cavity ; Phagocytes ; Respiratory Burst ; Sodium* ; Survival Rate

No abstract available.
Intracranial Aneurysm*

A new balloon dissector, a modification of a Foley catheter, was devised and it can be used either as a dissector or a tissue expander. Since most operating time was spent in balloon manipulation (inflation/deflation), the duct for saline injection was made to be wider than the Foley catheter. As a result, the balloon could be inflated faster than before. In order to reduce the scar at the donor site, harvest of the sural nerve using endoscopic technique is currently applied, but utilization of this method is technically difficult and requires a long operating time. For these reasons, new our method of using a balloon dissector was devised. The balloon dissector can also be used for immediate intraoperative tissue expansion for the reconstruction of small skin defects without distortion. We have found that the advantages of using the new balloon dissector include a reduction in operating time, preservation of the perforating vessels, and primary closure with less tension. In addition, this simple and inexpensive instrument is cost-beneficial to patients.
Catheters ; Cicatrix ; Humans ; Skin ; Sural Nerve ; Tissue Donors ; Tissue Expansion ; Tissue Expansion Devices

Granuloma gluteale infantum may be considered a complication oi the primary irritant type of diaper dermatitis and a modern disease due to the change in tae are of the napkin area. Most patients were infants who had worn plastic diapers and were treated with fluorinated topical corticasteroid cream for their diaper dermatitis. The lesions comorise one or several, soft and reddish brown or livid purple nodules on the napkin area. We report a typical case of granuloma gluteale infantum in a 13-month old female who had been treated with topical cortieosteroid crearn for her diaper derniat;lis. The granuloma regressed spontaneously after withdrawl of the topical corticosteroid and restricted use of the occlusive plastic diaper.
Dermatitis ; Female ; Granuloma* ; Humans ; Infant ; Plastics

Although the goal of fracture treatment is alive bony union with anatomical reduction, prosthetic replacement is indicated for some complicated femoral neck fracture. However it is not defined until now which one is selected between hemiarthroplasty and total hip replacement for the delayed or complicated femoral neck fracture. In order to get the criteria which one is better for this patient when indicated, we reviewed the result of 39 endoprosthetic and 16 total hip replacement patients at Seoul National University Hospital between June of 1975 to June of 1980. Both methods of endoprosthesis and total hip replacement showed satisfactory results of 72% and 90% respectively. Considering two points of the pain-relief and walking ability, total hip replacement is believed to be far better than endoprosthesis, especially for those patients who are over 50 years of age and have had more active living conditions before fracture.
Arthroplasty, Replacement, Hip ; Femoral Neck Fractures ; Femur Neck ; Femur ; Hemiarthroplasty ; Humans ; Joints ; Seoul ; Social Conditions ; Walking

No abstract available.
Humans ; Lymphocytes* ; Stomach Neoplasms* ; Stomach*

Primary tumors of the heart are rare. Half of all cardiac tumors are myxoma of which 20% are located in the right atrium. Especially multiple myxomas in the cardiac chamber were rarely reported. Clinically, myxomas present with various manifestations due to obstruction to blood flow, embolization,and constitutional changes. The association of pulmonary emboli and right atrial myxoma has been well documented but pulmonary emboli are more likely to be asymptomatic, misdiagnosed, or diagnosed late than peripherally-sited emboli. We report a case of multiple right atrial myxomas complicating pulmonary emboli in a 73-year-old man who had features of pulmonary embolism and was found to have multiple right atrial myxomas.
Aged ; Heart ; Heart Atria ; Heart Neoplasms ; Humans ; Myxoma* ; Pulmonary Embolism*