No abstract available.
Thrombectomy* ; Venous Thrombosis*

No abstract available.
Coronary Sinus*

Alginate gel is widely used as a scaffold in tissue engineering. Alginate solution has anionic properties, and calcium or magnesium cation has been used to crosslink alginate into a gel form. Chitosan not only has cationic properties but also is known to promote wound healing. Although there are some studies of chitosan- alginate gel use in drug delivery, reports of its application as a scaffold in tissue engineering are rare. The purpose of this study is to make chitosan-alginate gel and to investigate its biocompatibility as a scaffold for preadipocyte culture. 1, 2, 4, 6% chitosan solutions were mixed with 2% alginate solution to make various concentrations of chitosan-alginate gels. All of the gel which were made have been measured by Viscometer. Preadipocytes obtained from human breast fat tissue were seeded into each chitosan-alginate gel, and cell viability was measured by XTT colorimetric assay on the 2th, 4th, and 7th day of preadipocyte culture. The results of analysis were as follows. Each viscosity of 4% and 6% chitosan-alginate gels is similar to that of the calcium-alginate gel and 4% and 6% chitosan-alginate gels shows significantly higher cell viability than the calcium-alginate gel(p<0.05). In conclusion, chitosan-alginate gel is thought to be an appropriate scaffold for preadipocyte culture in tissue engineering.
Breast ; Calcium ; Cell Survival ; Chitosan ; Gels ; Humans ; Magnesium ; Tissue Engineering ; Viscosity ; Wound Healing

No abstract available.
Cardiopulmonary Bypass* ; Lipoproteins* ; Plasma*

BACKGROUND: Post-intubation injury is known to be the most common cause of tracheal stenosis. Treatment strategy for tracheal stenosis varies accoring to the extent of pathologic lesion. Focal mucosal lesion can be treated with laser photoablation, but full thickness tracheal lesion should be treated with resection and anastomosis. MATERIAL AND METHOD: From Aptil 1998 to May 1999, twelve patients suffering from tracheal stenosis as a complication of endotracheal intubation were managed by resection and end-to-end anastomosis in the Department of Thoracic and Cardiovascular Surgery, Gyeongsang National University Hospital. RESULT: There was no operative mortality. Five temporary vocal cord paralysis and one wound infection occurred as early complications. During 18 months of follow-up, re-stenosis was not found. CONCLUSION: Tracheal resection and anastomosis can be considered as an excellent surgical treatment for tracheal stenosis which developed as a complication of endotracheal intubation.
Follow-Up Studies ; Humans ; Intubation, Intratracheal ; Mortality ; Tracheal Stenosis* ; Vocal Cord Paralysis ; Wound Infection

Massive weight loss results in skin excess, leading to an unsatisfying body contour. Various thigh lift procedures can correct flabby skin in the lower leg. We present a lower body contouring technique with a report on two patients. The procedure is determined by the body contour of the patient. As the skin excess in the thigh area tended to appear mostly on the medial side, a vertical medial thigh lift was considered. Moreover, for patients with a pear/guitar-shaped body contour, we added the spiral thigh lift for skin excess in the buttocks and the lateral thigh area. The extent of tissue to excise was determined by pinching the patient in a standing position. The inferior skin flap was fixed to non-movable tissue, which was helpful for lifting the tissue and preventing the widening of the scar. After the operation, a drain was kept for 3 to 4 days. A compressive garment was used after removing the drain. There were no complications. The patients were discharged 6 to 8 days after the operation. In conclusion, skin excess, especially in the lower body, can be corrected by a thigh lift combining several procedures, varying from person to person.
Buttocks ; Cicatrix ; Humans ; Leg ; Lifting ; Skin ; Thigh ; Weight Loss

BACKGROUND: As the obese population increases in Korea, the number of patients who are trying to lose weight has been increasing steadily. In these patients, skin laxity and deformation of the body contour occurs, which could possibly be corrected by various body contouring surgeries. Here, we introduce the brachioplasty method and our experience of various body contouring surgeries performed in our center. METHODS: From November 2009 to August 2011, five cases of brachioplasty were performed. When the patient presented with sagging of the lateral inframammary crease and bat wing deformity in the axilla, extended brachioplasty was performed; in this case, the deformation of the axilla and lateral chest was corrected at the same time. A traditional brachioplasty was performed when contouring was needed only for skin laxity in the upper arm. RESULTS: Complications, such as hematomas or nerve injuries, were not evident. Some patients experienced partial wound dehiscence due to tension or hypertrophic scars found during the follow-up. In general, all of the patients were satisfied with the improvement in their upper arm contour. CONCLUSIONS: Given the demands for body contouring surgery, the number of brachioplasty surgical procedures is expected to increase significantly, with abdominoplasty comprising a large portion of these surgeries. For the brachioplasty procedure, preparation and preoperative consultation regarding design of the surgery by experienced surgeons was important to prevent complications such as nerve damage or hematoma formation.
Abdominoplasty ; Arm* ; Axilla ; Cicatrix, Hypertrophic ; Congenital Abnormalities ; Follow-Up Studies ; Hematoma ; Humans ; Korea ; Skin ; Surgery, Plastic ; Thorax ; Upper Extremity ; Weight Loss* ; Wounds and Injuries

No abstract available.

PURPOSE: Lipobean(R)s, widely used in lipodissolving techniques, contain phosphatidylcholine and sodium deoxycholate as its main substances. They have been approved only as medication for liver disease by the FDA. However, they have been used under various clinical settings without exact knowledge of its action mechanism. The authors designed an in vitro study to analyze the effects of different concentrations of phosphatidylcholine and sodium deoxycholate on adipocytes and other types of cells. METHODS: Human adipose-derived stem cell were cultured and induced to differentiate into adipocytes. Fibroblasts extracted from human inferior turbinate tissue, and MC3T3-E1 osteoblast lines were cultured. Phosphatidylcholine solution dissolved with ethanol was applied to the culture medium at differing concentrations (1, 4, 7, 10 mg/mL). The sodium deoxycholate solution dissolved in DMSO applied to the medium at differing concentrations (0.07, 0.1. 0.4. 0.7 mg/mL). Cells were dispersed at a concentration of 5 x 10(3) cells/well in 24 well plates, and surviving cells were calculated 1 day after the application using a CCK-8 kit. RESULTS: The number of surviving cells of adipocytes, fibroblasts and osteoblasts decreased as the concentration of sodium deoxycholate increased. However, all types of cells that had been processed in a phosphatidylcholine showed a cell survival rate of over 70% at all concentrations. CONCLUSION: This study shows that sodium deoxycholate is the more major factor in destroying adipocytes, and it is also toxic to the other cells. Therefore, we conclude that care must be taken when using Lipobean(R)s as a method of reducing adipose tissue, for its toxicity may destroy other nontarget cells existing in the subcutaneous tissue layer.
Adipocytes ; Adipose Tissue ; Cell Survival ; Deoxycholic Acid ; Dimethyl Sulfoxide ; Ethanol ; Fibroblasts ; Humans ; Liver Diseases ; Osteoblasts ; Phosphatidylcholines ; Sincalide ; Sodium ; Stem Cells ; Subcutaneous Tissue ; Turbinates

No abstract available.
Blepharoptosis* ; Dermoid Cyst* ; Humans