1.The action of Halothane and Isoflurane in Aortic Rings of Rabbit Pretreated with LNAME (L-nitrow arginine methyl ester) and MB (methylene blue).
Korean Journal of Anesthesiology 1997;33(1):6-14
BACKGROUND: The action of the volatile anesthetics on various organs in the body is not well known. Since Furchgott (1980) discovered endothelium derived relaxing factor (EDRF) from endothelium, many studies have been tried. Many investigators were studied about the relationship between the EDRF and the effect of the volatile anesthetics on blood vessels too. But the effect of volatile anesthetics and the site of action on the blood vessel were still controversial. In this study, we evaluated that the effect and the action site of halothane and isoflurane on isolated aortic rings of the rabbit. METHODS: Each of obtained thoracic aorta from rabbits (1.5~2.5 Kg) was divided into 3~5 mm sized rings, and a half of that were denuded. All of the aortic rings were preconstricted with phenylephrine 1.5 10-7 Mole in warm organ bath filled with modified Krebs' solution, and then LNAME (inhibitor of nitric oxide synthase, 3 10-4Mole) was administered to one group of aortic rings. MB (inhibitor of soluble guanylate cyclase, 2 10-5Mole) was administered to another one group and neither of LNAME nor MB was administered to the other group. And then isoflurane or halothane was administered (1~4%) to all of aortic rings. The polygraph recorded the changes of tension of aortic ring which was transmitted through the force transducer. RESULTS: It was proved that basal EDRF was released from endothelium by the fact that intact aortic rings were more constricted after LNAME or MB administration. The intact aortic rings were constricted in all concentration of isoflurane and both intact and denuded rings were relaxed in 4% concentration of halothane. CONCLUSION: It is concluded that isoflurane in all concentrations has an endothelium -mediated vasoconstriction and 4% halothane produced vascular relaxation directly independent of existence of the endothelium of vessel.
Anesthetics
;
Aorta, Thoracic
;
Arginine*
;
Baths
;
Blood Vessels
;
Endothelium
;
Endothelium-Dependent Relaxing Factors
;
Guanylate Cyclase
;
Halothane*
;
Humans
;
Isoflurane*
;
Methylene Blue
;
Nitric Oxide Synthase
;
Phenylephrine
;
Rabbits
;
Relaxation
;
Research Personnel
;
Transducers
;
Vasoconstriction
2.A clinical study on acute suppurative cholangitis.
Journal of the Korean Surgical Society 1992;42(4):485-492
No abstract available.
Cholangitis*
3.A clinical study on surgical thyroid nodules.
Hyeon Jung PARK ; Sang Eun MOON
Journal of the Korean Surgical Society 1993;45(6):920-928
No abstract available.
Thyroid Gland*
;
Thyroid Nodule*
4.Management of Neurogenic Fecal Incontinence and Constipation in Myelodysplastic Children by Malone Antegrade Colonic Enema Procedure: Early Experiences.
Hyeon JEONG ; Sung Eun JUNG ; Eun Sik LEE ; Kwang Myung KIM ; Sang Eun LEE ; Hwang CHOI
Korean Journal of Urology 2000;41(2):265-269
No abstract available.
Child*
;
Colon*
;
Constipation*
;
Enema*
;
Fecal Incontinence*
;
Humans
5.The Action of Enflurane and Desflurane in Aotic Rings of Rabbit Pretreated with LNAME (L-nitrow arginine methyl ester) and MB (methylene blue).
Gwui Bin KANG ; Eun Jung KWON ; Sang Ho LIM
Korean Journal of Anesthesiology 1997;33(4):583-590
BACKGROUND: Desflurane is a new inhaled anesthetic with the lowest blood/gas partition coefficient and enflurane is one of the major anesthetics in these days. But the effect of volatile anesthetics and the site of action on the blood vessel are still controversial. Since Furchgott (1980) discovered endothelium derived relaxing factor (EDRF) from endothelium, many investigators have studied about the relationship between the EDRF and the effect of the volatile anesthetics on blood vessels. In this study, we evaluated that the effect and the action site of enflurane and desflurane on isolated aortic rings of the rabbit. METHODS: Each of obtained thoracic aorta from rabbits (1.5~2.5 kg) was divided into 4~6 mm rings, and a half of that were denuded. All of the aortic rings were preconstricted with phenylephrine 1.5 10-7 Mole in warm organ bath filled with modified Krebs' solution, and then LNAME (inhibitor of nitric oxide synthase, 3 10-4Mole) was administered to one group of aortic rings. MB (inhibitor of soluble guanylyl cyclase, 2 10-5Mole) was administered to another one group and neither of LNAME nor MB was administered to the other group. And then enflurane (1%, 2%, 3%, 4%) or desflurane (6%, 9%, 12%) was administered to all of aortic rings. The polygraph recorded the changes of tension of aortic ring which was transmitted through the force transducer. RESULTS: It was proved that basal EDRF was released from endothelium by the fact that intact aortic rings were more constricted after LNAME or MB administration. The intact aortic rings were constricted in all concentration of enflurane and both intact and denuded rings were maintained from control tension in all concentrations of desflurane. CONCLUSION: It is concluded that enflurane in all concentrations has an endothelium-mediated vasoconstriction effect and desflurane in all concentrations has no effect on isolated aortic rings of rabbit.
Anesthetics
;
Aorta, Thoracic
;
Arginine*
;
Baths
;
Blood Vessels
;
Endothelium
;
Endothelium-Dependent Relaxing Factors
;
Enflurane*
;
Guanylate Cyclase
;
Humans
;
Nitric Oxide Synthase
;
Phenylephrine
;
Rabbits
;
Research Personnel
;
Transducers
;
Vasoconstriction
7.Two Case of Systemic Candidiasis in Premature Infants.
Dae Kyun KIM ; Woo Chul SUH ; Eun Gyeoung JUNG ; Eun Seok YANG ; Sang Kee PARK
Journal of the Korean Pediatric Society 1995;38(11):1558-1564
No abstract available.
Candidiasis*
;
Humans
;
Infant, Newborn
;
Infant, Premature*
8.Sequence Generation and Genotyping of 15 Autosomal STR Markers Using Next Generation Sequencing.
Eun Hye KIM ; Sang Eun JUNG ; Kyoung Jin SHIN ; Woo Ick YANG ; In Seok YANG
Korean Journal of Legal Medicine 2014;38(2):48-58
Recently, next generation sequencing (NGS) has received attention as the ultimate genotyping method to overcome the limitations of capillary electrophoresis (CE)-based short tandem repeat (STR) analysis, such as the limited number of STR loci that can be measured simultaneously using fluorescent-labeled primers and the maximum size of STR amplicons. In this study, we analyzed 15 autosomal STR markers via the NGS method and evaluated their effectiveness in STR analysis. Using male and female standard DNA as single-sources and their 1:1 mixture, we sequentially generated sample amplicons by the multiplex polymerase chain reaction (PCR) method, constructed DNA libraries by ligation of adapters with a multiplex identifier (MID), and sequenced DNA using the Roche GS Junior Platform. Sequencing data for each sample were analyzed via alignment with pre-built reference sequences. Most STR alleles could be determined by applying a coverage threshold of 20% for the two single-sources and 10% for the 1:1 mixture. The structure of the STR in each allele was accurately determined by examining the sequences of the target STR region. The mixture ratio of the mixed sample was estimated by analyzing the coverage ratios between assigned alleles at each locus and the reference/variant ratios from the observed sequence variations. In conclusion, the experimental method used in this study allowed the successful generation of NGS data. In addition, the NGS data analysis protocol enables accurate STR allele call and repeat structure determination at each locus. Therefore, this approach using the NGS system will be helpful to interpret and analysis the STR profiles from singe-source and even mixed samples in forensic investigation.
Alleles
;
DNA
;
Electrophoresis, Capillary
;
Female
;
Gene Library
;
Humans
;
Ligation
;
Male
;
Microsatellite Repeats
;
Multiplex Polymerase Chain Reaction
;
Statistics as Topic
9.A Case of Eosinophilic Cellulitis in a Child.
Jung Eun SEOL ; In Ho PARK ; Wonkyung LEE ; Hyojin KIM ; So Young JUNG ; Sang Hyun KIM
Korean Journal of Dermatology 2015;53(5):409-410
No abstract available.
Cellulitis*
;
Child*
;
Eosinophils*
;
Humans
10.A Case of Anterior Cervical Hypertrichosis.
Jung Eun LEE ; Mi Jung LEE ; Sang Ju LEE ; Seung Hun LEE
Annals of Dermatology 2004;16(3):132-133
No abstract available.
Hypertrichosis*