Between 1984 and 1996, 8 patients who were suffered from stage g Kienbock's disease underwent interpositional arthroplasty using pronator quadratus pedicled bone. Patients comprised 2 males and 6 females, with an average age of 33 years(range 19-47). Range of motion of the wrist, residual pain, grip strength, and carpal height ratio of the patients were analyzed. The follow-up period was between 1.5 years and 12.5 years(average 5.5 years). The arc of flexion-extension of the wrist was increased from average 74 degrees preoperatively to 96 degrees postoperatively. Among eight patients, five were free of pain and three had intermittent pain during heavy work. None of eight patients had any discomfort in daily work and changed his or her occupation. The grip strength was average 83% of the normal side. Carpal height ratio was average 0.48 preoperatively and was not changed postoperatively. Clinical results, assessed by Lichtman-Evans criteria, showed 4 good and 4 fair. The interpositional arthroplasty using pronator quadratus pedicled bone was considered as a very effective method for the treatment of stage III Kienbock's disease.
Arthroplasty* ; Female ; Follow-Up Studies ; Hand Strength ; Humans ; Male ; Occupations ; Osteonecrosis* ; Range of Motion, Articular ; Wrist

The 38 kDa protein of Mycobacterium tuberculosis, which was known previously as antigen 5, has been extensively used in the serodiagnosis of tuberculosis. In an attempt to develop and evaluate a serodiagnostic test using the antigen, we expressed the 38 kDa protein in BCG and its seroreactivity was compared to that expressed in Escherichia coli. The coding region of the 38 kDa protein was amplified by PCR, and the gene was cloned into a Mycobacterium-E. coli shuttle expression vector pYMC-his and pQE30 expression vector and expressed in BCG and E. coli, respectively. Both recombinant 38 kDa proteins showed strong seroreactivity against pooled serum from tuberculosis patients. There was no significant difference in seroreactivity between the two recombinant antigens in sera from the far advanced tuberculosis patients. However, of 25 tuberculosis patients graded as ""minimal"" by chest X-ray, 5 (20.0%) were seropositive by r38 kDa expressed in E. coli, while 8 (32.0%) by that expressed in BCG. Likewise, higher seroreactivity by r38 kDa expressed in BCG was found in sera from the moderately advanced tuberculosis. This study thus indicates that the recombinant 38 kDa expressed in BCG is more effective than that expressed in E. coli in detecting antibodies to the native 38 kDa protein of M. tuberculosis in sera from minimally affected tuberculosis patients.
Antibodies ; Clinical Coding ; Clone Cells ; Escherichia coli ; Humans ; Mycobacterium bovis* ; Mycobacterium tuberculosis* ; Mycobacterium* ; Polymerase Chain Reaction ; Serologic Tests* ; Thorax ; Tuberculosis*

The ability to introduce recombinant DNA molecules back into mycobacteria would greatly increase the potential of molecular genetic approaches for the study of mycobacteria as well as for the use in clinical purposes. We have initiated the construction of vectors that facilitates the introduction of recombinant DNA into mycobacteria. The vector was designed to contain replicons for multiplication in mycobacteria and Escherichia coli, a promoter for gene expression, a drug resistant gene for selecting transformants, and a few restriction enzyme sites for convenient cloning. Constructed Mycobacterium-E. coli shuttle vector named p YMC (hsp60) was shown to transform M. smegmatis at high efficiency and maintain plasmid at stable level. The ability of the vector to express cloned foreign gene was also monitored by measuring the expressed level of luciferase gene which was used as a reporter. High level of luciferase activity in M. smegmatis with pYMC (hsp60:luc) was detected confirming successful construction of Mycobacterium-E. coli shuttle vector.
Clone Cells ; Cloning, Organism ; DNA, Recombinant ; Escherichia coli* ; Escherichia* ; Gene Expression ; Genetic Vectors* ; Luciferases ; Molecular Biology ; Mycobacterium* ; Plasmids ; Replicon

No abstract available.
Blister* ; Fibroma*

PURPOSE: The aim of our study was to evaluate the usefulness of MR imaging in cases of traumatic brachial plexus injury. MATERIALS AND METHODS: We evaluated 25 patients with traumatic brachial plexus injury as seen on MR images prior to surgical exploration and repair. MR images were retrospectively evaluated for nerve root avulsion and pseudomeningocele, and postganglionic lesions. Results were correlated with final diagnosis after surgical exploration . RESULTS: One hundred and four of 125 root levels (83%) were adequately imaged. Neve root avulsion was shown at 28 levels(54%). Avulsion with or without pseudomeningocele was seen at 37 levels(71%)(80% sensitivity, 91% specificity). The presence of ten of 12 postganglionic lesions (83%) was revealed by MR imaging. CONCLUSION: MR imaging is valuable for revealing preganglionic nerve root avulsion in patients with traumatic brachial plexus injury or postganglionic lesions.
Brachial Plexus* ; Diagnosis ; Humans ; Magnetic Resonance Imaging* ; Radiculopathy ; Retrospective Studies

PURPOSE: The aim of our study was to evaluate the usefulness of MR imaging in cases of traumatic brachial plexus injury. MATERIALS AND METHODS: We evaluated 25 patients with traumatic brachial plexus injury as seen on MR images prior to surgical exploration and repair. MR images were retrospectively evaluated for nerve root avulsion and pseudomeningocele, and postganglionic lesions. Results were correlated with final diagnosis after surgical exploration . RESULTS: One hundred and four of 125 root levels (83%) were adequately imaged. Neve root avulsion was shown at 28 levels(54%). Avulsion with or without pseudomeningocele was seen at 37 levels(71%)(80% sensitivity, 91% specificity). The presence of ten of 12 postganglionic lesions (83%) was revealed by MR imaging. CONCLUSION: MR imaging is valuable for revealing preganglionic nerve root avulsion in patients with traumatic brachial plexus injury or postganglionic lesions.
Brachial Plexus* ; Diagnosis ; Humans ; Magnetic Resonance Imaging* ; Radiculopathy ; Retrospective Studies

No abstract available.
Antibodies* ; Borrelia burgdorferi* ; Borrelia* ; Immunoblotting* ; Lyme Disease*

No abstract available.
Neurilemmoma* ; Toes*

No abstract available.
Nipples*

No Abstract Available.
Brucella abortus* ; Brucella* ; Immunity, Humoral* ; Jeju-do* ; Recombinant Proteins*