No abstract available in English.
Tibia

Interaction with Candida albicans (C. albicans) causes secretion of a variety of cytokines by macrophages. Although macrophages and neutrophils are thought to be major effector cell types in inflammation, fibroblasts have also been shown to participate in a wide array of inflammatory reactions. The patterns of cytokine gene (IL-4, IL-6, IL-10) expression have been examined in NIH 3T3 fibroblasts (NIH 3T3) and thioglycollate-elicited murine peritoneal macrophages (macrophages) in response to Candida spp. (C. albicans and C. tropicalis) and compared with NIH 3T3 and macrophage cells stimulated with lipopolysaccharide (LPS) by using RT-PCR. Active and heat inactivated (100'C, 30min) C. albicans and C. tropicalis were used at 1:10 (macrophages or NIH 3T3: yeasts ratio) concentration as a stimulators. Active and inactivated forms of Candidn spp. induced same patterns of cytokine gene expression on macrophages or NIH 3T3 cells. IL-6 mRNA was induced on both macrophages and fibroblasts, but IL-4 mRNA was not induced on both of them. Howerver, IL-10 mRNA expression was observed differently in that it was expressed in macrophages but not in fibroblasts. C. albicans-induced cytokine mRNA expression were same to C. tropicalis-induced one. C. albicans or C. tropicalis-induced cytokines gene expression (IL-4, IL-6, IL-10) showed same patterns to LPS-induced one. These observation provide that, although C. albicans and C. tropicalis have different pathogenic activity, they can induce the expression of Th2 cell-derived cytokine genes with same patterns, and IL-10 gene expression may be in a cell type specific manner. But further experiment with various kinds of fibroblasts is needed to determine cell type-specific manner in C. albicans or C. tropicalis-induced IL-10 gene expression.
Animals ; Candida albicans ; Candida* ; Cytokines ; Fibroblasts* ; Gene Expression* ; Hot Temperature ; Inflammation ; Interleukin-10 ; Interleukin-4 ; Interleukin-6 ; Macrophages ; Macrophages, Peritoneal* ; Mice* ; Neutrophils ; NIH 3T3 Cells ; RNA, Messenger ; Yeasts

A patient with glioblastoma multiforme was treated with postoperative radiotherapy and a simultaneous induction chemotherapy with CCNU(75mg/sqm), procarbazine(100mg/sqm), vincristine(1.4mg/sqm). Follow up C-T scanning showed a mild decreased density area of left frontal lobe and the patient was discharged without any evidence of neurological deficit except mild decreased visual acuity.
Drug Therapy* ; Follow-Up Studies ; Frontal Lobe ; Glioblastoma ; Humans ; Induction Chemotherapy ; Radiotherapy ; Visual Acuity

OBJECTIVE: The purposes of the present study were to investigate the effect of gamma-radiation on the expression of inhibin-alpha proteins and genes for inhibin alpha, betaA, and betain the ovary. METHODS: Immature mice were whole-body gamma-irradiated with 25% of a lethal dose. At time 0, 3, 6, 12, and 24 hours after the irradiation,the ovaries were collected and used for immunohistochemistry for inhibin-alpha, and RT_PCR for inhibin-alpha, betaA, and betaB. RESULTS: The expression of the immunoreactive inhibins-alpha was maintained at 12 hours post-irradiation and reduced thereafter. The expression of inhibin-alpha mRNA was significantly increased with the time after the irradiation. However there were no significant changes in the expression of betaA and betaB mRNAs. CONCLUSION: It might be thought that inhibin acts as one of the regulatory factors in the gamma-radiation-induced follicular atresia in mice
Animals ; Female ; Follicular Atresia ; Immunohistochemistry ; Inhibins* ; Mice* ; Ovary* ; RNA, Messenger

A choroid detachment occurs most commonly after surgical operation for cataract, glaucoma, and retinal detachment. It is fairly characteristic for postoperative choroidal edema to be accompanied by hypotonia and a shallow anterior chamber. We have recently experienced 3 cases of choroid detachment after cataract operation, and acase of choroid detachment combined with retinal detachment after trabeculectomy. They were also accompanied by hypotonia and shallow anterior chamber, but had not would leakage. Among 4 cases, a case of choroid detachment was performed suprachoroidal tapping, the remaining were treated with prednisolone, cycloplegics, and pressure dressing. They were not of consequence and subsided without being noticed. A brief review of relating literatures was described.
Anterior Chamber ; Bandages ; Cataract ; Choroid* ; Edema ; Glaucoma ; Muscle Hypotonia ; Mydriatics ; Prednisolone ; Retinal Detachment ; Trabeculectomy

Osteochondromadevelop most commonly at distal femur, proximal humerus and proximal tibia, but the rib osteochondroma was reported less commonly. In this report, scapular snapping syndrome complicated by adventitious bursa and soft tissue pseudotumor surrounding the osteochondroma of the 6th rib body was treated successfully by surgical excision of them. We report this rare case with reviewing the relevant literature.
Femur ; Humerus ; Osteochondroma* ; Ribs* ; Shoulder* ; Tibia

We had precise and accurate information concerning the position of the foreign body, its nature, and the extent of damage to the eye. A linear half-thickness incision which was paralleI to the limbus was made on the sclera immediately external to the location of the foreign body. A lamellar scleral undermining was performed on the both sides of a single scleral incision. Diathermy was applied in the area undermined. A T-shaped incision went all the way through the lamellar scleral bed, cleanly eXIo3ing the dark uveal tissue. The hand magnet was brought on the exposed choroid; the bulging of the choroid was obvious proof of its presence. And then gentle to-and-fro scratching with a nonmagnetic spatula on the bulging uveal tissue caused the foreign body to be extracted easily. The T-shaped incised scleral bed was closed. The preserved human sclera was burried in the lamellar dissected sclera. Practically any portion of the sclera up to a few millimeters in front of the optic nerve head can be approached. There were significant advantages to these procedures such as a high successful rate, a sufficiently promising effect in prophylaxis and treatment of the retinal detachment. Among 15 cases of the magnetic lOPB. including 7 cases of combined retinal detachments. 14 cases of IOFB and all cases of the retinal detachment showed to be completely recovered. The procedure failed in 1 case, in which foreign body located and adhered firmly on disc.
Choroid ; Diathermy ; Foreign Bodies* ; Hand ; Humans ; Optic Disk ; Retinal Detachment ; Sclera ; Scleral Buckling*

PURPOSE: The mechanism by which BCG exerts its antitumor response in superficial bladder cancer is unclear but associated with T cell mediated immunity and cytokine production. The type 1 helper T cells(Th1) produce interleukin-2(IL-2) and interferon-gamma(IFN-gamma) while type 2 helper T cells (Th2) release interleukin-4(IL-4), interleukin-5(IL-5) and interleukin-10(IL-10). We measured cytokines(IL-12, IFN-gamma and IL-10) to determine if BCG induced the production of Th1 and Th2 cytokines from urine and serum. MATERIALS AND METHODS: In this study 10 patients with superficial bladder cancer(Ta, T1), 5 normal volunteers and 5 patients with cystitis were evaluated. Urine and serum were collected before BCG and during 4-6 hours after 5 or 6 consecutive weekly BCG instillations. The cytokines were measured by monoclonal ELISA assays. RESULTS: Urine and serum levels of IL-12, IFN-gamma and IL-10 were no difference between control groups(normal volunteers and cystitis patients) and pre-BCG bladder cancer patients. There were no apparent elevations of IFN-gamma and IL-10 levels in the sera following BCG therapy except for IL-12. In 8 patients following BCG therapy, IFN-gamma levels were significantly elevated in the urine compared to pre-BCG patients. IL-12 and IL-10 were also significantly increased in the urine in 7 and 6 patients respectively. Elevations of IFN-gamma in the urine correlated most with that of IL-12 and IL-10. CONCLUSIONS: Our results demonstrate that BCG can induce the production of Th1 and Th2 cytokines from urine and these cytokines may contribute to the antitumor immunity of BCG. However, further study is necessary to identify the correlation between change of these cytokines and clinical outcome such as recurrence or progression.
Bacillus* ; BCG Vaccine ; Cystitis ; Cytokines* ; Enzyme-Linked Immunosorbent Assay ; Healthy Volunteers ; Humans ; Immunity, Cellular ; Interleukin-10 ; Interleukin-12 ; Mycobacterium bovis ; Recurrence ; T-Lymphocytes, Helper-Inducer ; Urinary Bladder Neoplasms* ; Urinary Bladder* ; Volunteers

A newly developed third generation enzyme immunoassay(Lucky HCD 3.0 EIA) for hepatitis C virus(HCV) antibodies was added with the envelope(E1E2)/NS4 fusion proteins and expanded NS5 proteins as well as the core/NS3 fusion proteins. Authors evaluated the HCD 3.0 EIA with the previously available second generation EIA(HCD 2.0) in 10,435 Red Cross blood donors. Among 10,435 donors who were screened for the presence of HCV antibodies by HCD 2.0 assay, 22(0.21%) sera were repeatedly reactive. All of these sera were tested for further testing. Only 13 of all tested sera were reactive by HCD 3.0 EIA, and nine sera were not reactive. Nine of 13 HCD 3.0 positive sera were reactive by recombinant immunoblot assay(Lucky-Confirm). Also seven of these 13 sera had detectable HCV genomic RNA by reverse transcriptase-polymerase chain reaction(RT-PCR). None of nine HCD 3.0 negative samples had detectable immunoblot assay and HCV genomic RNA. It is concluded that the new HCV EIA can decrease a significant false positivity of second generation EIA in a blood donor population. This new assay correlates well with detection of HCV-RNA by RT-PCR and identifies donors who are truly infected.
Antibodies ; Blood Donors* ; Hepatitis C ; Hepatitis C Antibodies ; Humans ; Immunoblotting* ; Red Cross ; RNA ; Tissue Donors

"Background: In using the Double-lumen tube (DLT), knowing the minimum bronchial cuff volume (MCV) for an effective air-tight seal will be useful; to provide a collapse of the lung; and to avoid pressure damage. The aims of the present study are thus three-fold: to measure the MCV; to measure the diameter of left main bronchus (LMBD); and to prove any relationships between two parameters. METHODS: One hundred men and forty women who needed intubation of left-sided DLT were enrolled in this study. 37 Fr DLTs were used in male patients, and 35 Fr DLTs were used in female patients. We evaluated the MCV by air bubble method and measured the LMBD in chest PA. We also evaluated the pressure/volume characteristics of the bronchial cuffs by control inflator. RESULTS: 29 patients of 100 patients (29%) exhibited persistent air leakage in 2.5 ml cuff volume in male patients (group of MCV >2.5). On the contrary, 18 patients of 40 patients (45%) did not require any cuff volume in female patients (group of MCV 0). The mean LMBD were 13.23 1.45 mm in male and 11.09 0.96 mm in female. There were significant positive correlations between MCV and LMBD in both sex and their respective correlation coefficients were 0.264 (P=0.008) in male and 0.484 (P=0.002) in female. The equations of linear regression were: LMBD = 12.394 0.429xMCV in male, LMBD = 10.725 0.438xMCV in female. CONCLUSIONS: The MCV of the brochial cuffs in left-sided DLTs has significant relationships with the LMBD measured in chest PA.
Bronchi ; Female ; Humans ; Intubation ; Linear Models ; Lung ; Male ; Thorax