OBJECTIVE: In the previous study, we compiled the differentially expressed genes during early folliculogenesis.1 Objective of the present study was to identify downstream target genes of transcription factors (TFs) using bioinformatics for selecting the target TFs among the gene lists for further functional analysis. MATERIALS AND METHODS: By using bioinformatics tools, constituent domains were identified from database searches using Gene Ontology, MGI, and Entrez Gene. Downstream target proteins/genes of each TF were identified from database searches using TF database (TRANSFAC(R) 6.0) and eukaryotic promoter database (EPD). RESULTS: DNA binding and trans-activation domains of all TFs listed previously were identified, and the list of downstream target proteins/genes was obtained from searche of TF database and promoter database. Based on the known function of identified downstream genes and the domains, 3 (HNF4, PPARg, and TBX2) out of 26 TFs were selected for further functional analysis. The genes of wee1-like protein kinase and p21WAF1 (cdk inhibitor) were identified as potential downstream target genes of HNF4 and TBX2, respectively. PPARg, through protein-protein interaction with other protein partners, acts as a transcription regulator of genes of EGFR, p21WAF1, cycD1, p53, and VEGF. Among the selected 3 TFs, further study is in progress for HNF4 and TBX2, since wee1-like protein kinase and cdk inhibitor may involved in regulating maturation promoting factor (MPF) activity during early folliculogenesis. CONCLUSIONS: Approach used in the present study, in silico analysis of downstream target genes, was useful for analyzing list of TFs obtained from high-throughput cDNA microarray study. To verify its binding and functions of the selected TFs in early folliculogenesis, EMSA and further relevant characterizations are under investigation.
Computational Biology ; Computer Simulation* ; DNA ; Gene Ontology ; Maturation-Promoting Factor ; Oligonucleotide Array Sequence Analysis ; Protein Kinases ; Transcription Factors* ; Vascular Endothelial Growth Factor A

No abstract available.
Endolymphatic Hydrops*

No abstract available.
Female ; Ovulation*

No abstract available.
Malingering*

The femoral neck fracture having concomitant fracture of the femoral shaft occurs predaminantly in young man and have known to associate the serious complications. One of such factures is sometimes overlooked and is possibly due to a more obvious nature of the shaft fracture than that of the ipsilateral neck. Six cases of this type of combined fractures in ipsilateral femur are reported with the review of 1 iteratures. In six cases of the femoral neck fractures, three which were unrecognized at initial examiaation in early phase, resulting in serious residue as much as deformity and avascular necrosis of the femoral head, and other three were recognized in early at initial examination healed in good anatomic position and in due time. Through this observation it is stressed that early discovery and adaquate treatment of the fracture is the only solution to minimize the sequelae.
Congenital Abnormalities ; Femoral Neck Fractures ; Femur ; Femur Neck ; Head ; Neck ; Necrosis

22 cases of giant cell tumor of bone treated between 1973 and 1979 have been reviewed after a miaimum follow-up of two years and following results were obtaind. 1. Fourteen cases (63%) were in the third and fourth decade. 2. Fourteen cases (63%) were located around the knee joint. 3. Five (42%) of the twelve tumors treated by curettage and bone graft recurred. Five tumors treated by En bloc resection and two tumors treated by amputation had no recurrences. 4. Five of six recurrences occured within two years after mitial treatment.
Amputation ; Curettage ; Follow-Up Studies ; Giant Cell Tumor of Bone ; Giant Cell Tumors ; Giant Cells ; Knee Joint ; Recurrence ; Transplants

No abstract available.
Candida albicans* ; Candida* ; Dentures* ; Detergents*

No abstract available.
Extraembryonic Membranes* ; Placenta*

No abstract available.
Female ; Mammals* ; Ovulation* ; Rupture*

No abstract available.
Female ; Ovary* ; Ultrasonics*