Vibrio vulnificus was isolated from a blood culture of a 41-year-old male patient with liver cirrhosis. He had eaten raw fish one day prior to the onset of clinical symptoms which included fever, chills, diarrhea and hypotension. He also developed cellulitis of the right leg which developed into a necrotic ulcer. The isolate was a slightly curved gram-negative bacillus and the colony morphology on a TCBS plate was similar to that of V. parabaemolyticus. Acid production from lactose was detected after 2 days of incubation. Other biochemical tests showed typical reactions of V vulnificus. The isolate was susceptible to all of the tested antibiotics except to clindamycin, colistin and penicillin G.
Adult ; Human ; Liver Cirrhosis/complications* ; Male ; Microscopy, Electron ; Septicemia/microbiology* ; Vibrio/isolation & purification ; Vibrio/ultrastructure ; Vibrio Infections/microbiology*

When conducting an exposure assessment, the primary goal of the industrial hygienist is to fully characterize the worker's exposure during a work shift to compare it with an occupational exposure limit. This applies regardless of the duration of the work activity as an activity that is relatively short in duration can still present exposure in excess of the occupational exposure limit even when normalized over an 8-hr shift. This goal, however, is often impeded by the specification of a minimum sample volume in the published sampling method, which may prevent the sample from being collected or submitted for analysis. Removing the specification of minimum sample volume (or adjusting it from a requirement to a recommendation), in contrast, allows for a broader assessment of jobs that consist of short-duration and high-exposure activities and also eliminates the unnecessary practice of running sampling pumps in clean air to collect a specified, minimum volume.
Methods ; National Institute for Occupational Safety and Health (U.S.) ; Occupational Exposure ; Occupational Health ; Running