Objective:To evaluate the activity of spiromesifen against the most abundant and investigated mosquito species,Culiseta longiareolata Aitken,1954 (Diptera,Culicidae).Methods:Culiseta longiareolata larvae were collected from untreated areas located at Tébessa (Northeast Algeria).A commercial formulation of spiromesifen (Oberon(R) 240 SC) was tested at different concentrations ranging between 238 and 1428 μg/L on newly molted fourth-instar larvae under standard laboratory conditions according to Word Health Organization recommendations.The effects were examined on the mortality,the morphometdc measurements,two biomarkers (catalase and malondialdehyde),and the biochemical composition of larvae,respectively.Results:The compound exhibited insecticidal activity.Moreover,it disturbed growth and several morphological aberrations were observed.It also affected body volume,biomarkers and contents of carbohydrates,lipids and proteins.A marked effect on lipids and malondialdehyde was noted,confirming its primary mode of action on lipid synthesis.Conclusions:Spiromesifen appears less potent than other insecticides tested such as the insect growth disruptors.

Objective To evaluate the activity of spiromesifen against the most abundant and investigated mosquito species, Culiseta longiareolata Aitken, 1954 (Diptera, Culicidae). Methods Culiseta longiareolata larvae were collected from untreated areas located at Tébessa (Northeast Algeria). A commercial formulation of spiromesifen (Oberon

A study was performed to estimate the prevalence of the external bacterial flora of two domestic cockroaches (Blattella germanica and Blatta orientalis) collected from households in Tebessa (northeast Algeria). Three major bacterial groups were cultured (total aerobic, enterobacteria, and staphylococci) from 14 specimens of cockroaches, and antibiotic susceptibility was tested for both Staphylococcus and Pseudomonas isolates. Culturing showed that the total bacterial load of cockroaches from different households were comparable (P<0.001) and enterobacteria were the predominant colonizers of the insect surface, with a bacterial load of (2.1 × 10⁵ CFU/insect), whereas the staphylococci group was the minority. Twenty-eight bacterial species were isolated, and susceptibility patterns showed that most of the staphylococci isolates were highly susceptible to chloramphenicol, gentamycin, pristinamycin, ofloxacin, clindamycin, and vancomycin; however, Pseudomonas strains exhibited resistance to amoxicillin/clavulanic acid, imipenem, and the second-generation antibiotic cephalosporin cefuroxime.
Algeria ; Animals ; Blattellidae ; microbiology ; Drug Resistance, Bacterial ; Female ; Housing ; Male ; Microbiota ; Pseudomonas ; drug effects ; Staphylococcus ; drug effects