Eighty-two strains of Escherichia coli isolated from urine specimens in Pusan University Hospital, were serotyped and analyzed for plasmid DNA profiles, PFGE profiles, MRHA of human blood cells, HEp-2 cell adherence ability and reactivity to bfpA, LT, STh and STp DNA probes. The following results were obtained. Fifty-three of the eighty-two strains belonged to thirteen different 0 serotypes, twenty-nine strains could not be typed with the antisera used. Thirty strains (43.9%) were hemolysin producer. MRHA is present on twenty-nine strains (35.37%) of eighty-two strains. MRHA positive strains carry a plasmid of 60MDa, a putative factor involved in adherence. This plasmid might be specific for MRHA positive strains. MRHA positive strains were observed in serotype 01, 018, 055, 086a, 0119, 0126, and 0142. Twenty-six strains of E. coli showed three patterns of adherence to HEp-2 cells namely, localized, diffuse, and aggregative adhesion. Twenty-two strains hybridized with the bfpA probe, while all eighty-two strains did not hybridize with the probes, LT, STh, STp. The restriction fragment patterns of chromosomal DNA digested with AotI analysed by PFGE of hemolysin-producing E. coli ten strains were compared with eight different types. Three of E. coli serotype 01, 08 and 0126 showed the same chromosomal DNA fragment patterns.
Blood Cells ; Busan ; DNA ; DNA Probes ; Escherichia coli* ; Escherichia* ; Humans ; Immune Sera ; Plasmids ; Serotyping*

BACKGROUND: The reproducibility in grading a cervical intraepithelial neoplasia (CIN) are not perfect. The aim of this study was to assess the value of the immunohistochemical expression of p63 and the other biomarkers for grading a CIN (dysplasia and in situ carcinoma), and diagnosing invasive carcinomas. METHODS: Sixty six cervical specimens were immunostained with the monoclonal antibodies against p63, Ki-67, p27Kip1, and p53 to determine the localization. RESULTS: The p63 positive cells are well linked with squamous cell maturation and the degree of dysplasia. In mild dysplasia, the p63 positive cells were localized to the basal and parabasal cells, which gradually extended into the middle and upper layers in moderate and severe dysplasia. p63 expression was strong in immature squamous epithelium and invasive squamous cells, but was constantly absent in an adenocarcinoma. The Ki-67 positive cells were scattered from the parabasal cells to the superficial cells in accordance with the degree of dysplasia. p27Kip1 expression was noted in the intermediate cells in the normal cervix. In CIN, the p27Kip1 positive nuclei tended to extend to the basal cells, but it showed no diagnostic consistency in an invasive carcinoma. p53 expression was also variable. CONCLUSION: p63 is a useful diagnostic adjunct for grading CIN as well as for detecting microinvasion and squamous differentiation in invasive carcinoma. However, immunohistochemical expressions for the p27Kip1 and p53 have no correlation with the grade of CIN and squamous cell carcinoma.
Adenocarcinoma ; Antibodies, Monoclonal ; Biomarkers ; Carcinoma, Squamous Cell ; Cervical Intraepithelial Neoplasia ; Cervix Uteri ; Epithelium ; Female ; Uterine Cervical Neoplasms

No abstract available.
Arthrogryposis*

No abstract available.
Cervix Uteri* ; Female ; Magnetic Resonance Imaging*

No abstract available.

After the immunization of pigmented guinea pigs with bovine rod outer segments in complete Freund's adjuvant, the regional lymph nodes were excised, teased and sedimented by centrifugation. These fresh lymphocytes were injected intravitreally (a single injection of 10 X 10 cells in 50 micro l) in 18 eyes. The involvement of the ciliary body and the choroid with polymorphonuclear leucoytes and later with round cells was noted in 16 eyes. Destructions of the visual cells of the retina were demonstrated in 8 eyes. When the fresh lymphocytes were injected subconjunctivally (50 X 10 cells in 30 micro l) four times. four of the 8 eyes showed slight infiltrations of the ciliary body and the choroid with round cells. No retinal lesion was found. Following repeated freezing and thawing of the lymphocytes. transfer experiments wereperformed with the nonviable cells and their extracts respectively. In 8 eyes injected intravitreally with nonviable cells. 2 eyes showed the infiltration of the uvea with round cells, whereas. of 6 eyes injected subconjunctivally, only one eye showed such changes. The extracts of lymphocytes were injected intravitreally in 8 eyes of which 5 eyes demon-strated moderate degree of infiltration of the uvea. Of 2 eyes injected subconjunctivally, 2 eyes. showed slight histological changes. As control experiments, lymphocytes taken from animals immunized. only with the adjuvant were treated as before and transferred to normal animals. Only one eye of 3 eyes received single intravitreal injection of fresh cells showed slight degree of infiltration of the uvea. Thus it can be concluded that the experimental autoimmune uveitis in guinea pigs could be transferred to normal animals by the transfer of non-viable lymphocytes and especially by their extracts.
Animals ; Centrifugation ; Choroid ; Ciliary Body ; Freezing ; Freund's Adjuvant ; Guinea Pigs ; Immunization ; Intravitreal Injections ; Lymph Nodes ; Lymphocytes ; Retina ; Retinaldehyde ; Rod Cell Outer Segment ; Uvea ; Uveitis*

No abstract available.

In order to investigate the effects of diathermy and cryosurgery on the scleral shrinkage, nonperforating surface diathermy and cryocautery were applied in rabbits eyes. The premarked scleral segments were measured by corneal microscope with micrometer, and the resultant elevation of the intraocular pressures was pressures was measured by Schiotz tonometer. In the eyes treated by 10 diathermy in 5 X 3mm area, average 20.1% of scleral shrinkage was observed. There was no significant difference between shrinkages in the meridional and latitudinal directions. In the eyes treated by cryopencil no significant changes were found. Diathermy applications in 3 X 10mm area in the sclera induced 36.9% increase in the intraocular pressure which lasted about 9 minutes. On the other hand, cryoapplication did not produce such an elevation in ocular tension.
Cryosurgery ; Diathermy* ; Hand ; Intraocular Pressure ; Rabbits* ; Sclera

No abstract available.
Primary Myelofibrosis*

A case, 25-year-old male student who is thought to have been suffered from glaucomatocyclitic crises in the left eye, is presented. Authors have followed this patient for about one and a half years. During the time, 10 times of recurrent unilateral attacks of ocular hypertension have been noted with assoicated mild iridocyclitis. An attempt was made to evaluate the clinical pictures and review the literatures.
Adult ; Humans ; Iridocyclitis ; Male ; Ocular Hypertension