No abstract available.
Animals* ; Leptospirosis*

No abstract available.
Animals* ; Leptospirosis*

There are various complex formations between immunoglobulin and serum enzymes in the body. Of these, LD-Immunoglobulin complex is most common. It has reported that LD-IgG or LD-IgA complex existed in the serum of healthy person or many patients who have various clinical disorders. The clinical significance of complexes is not known, but it results in the increase of total LD activity and interference to the LD isoenzyme electrophoresis interpretation due to its anomalous pattern. We have reported a case of LD-Immunoglobulin complex in AILD(Angioimmunoblastic lymphadenopathy with dysproteinemia) with reference. The patient was admitted with fever, chills and cervical LN enlargement, in LN biopsy, diagnosed AILD, and during treatment expired by sepsis. Since admission, serum total LD activities were increased and serum LD isoenzyme EP showed that LD3-5 fractions was not separated in broad single band. Its pattern was due to LD-immunoglobulin complex, LD-bound immunoglobulin was IgG ,lamda. For the identification of immunoglobulin, we at first did serum protein immunoelectrophoresis. then stained the plate with tetrazolium dye for LD activity.
Biopsy ; Chills ; Electrophoresis ; Fever ; Humans ; Immunoblastic Lymphadenopathy* ; Immunoelectrophoresis ; Immunoglobulin G ; Immunoglobulins ; Lactic Acid* ; Lymphatic Diseases ; Sepsis

No abstract available.
Humans ; Muramidase*

No abstract available.
Humans ; Muramidase*

No abstract available.
Humans ; Muramidase*

Thelazia callipaeda is a slender, long, and cylindrical nematode which parasitizes in the conjunctival sac of human and causes conjunctivitis. The animals such as the dog, rabbit, horse, deer, and cow were revealed as its reservoir and some species of the fly suspected as its vector. We experienced a case of T. callipaeda isolated from human conjunctival sac of a 41-year old man who lived in Wanju-gun, Chonbuk province and raised the dogs. He complained of an irritation, itching and foreign body sensation on his right eye and the two worms were picked out of his right eye by forceps from conjunctival sac. General features of the worms were ivory colored and slender. Two worms were 15.2mm and 15.8mm in length and both have less than 1.0mm in maximum width. Microscopically, both of the worms were female. The vulva opening of the worms located anterior to esophago-intestinal junction. The uterus filled with the eggs an6 larvae encysted with oval membrane. The buccal cavity in head portion was tetrazoid and connected with well-developed esophagus. At the tails of the worms, anus and papillae were observed. Characteristic compact cuticular transverse striations were identified on the whole body surface.
Adult ; Anal Canal ; Animals ; Conjunctivitis ; Deer ; Diptera ; Dogs ; Eggs ; Esophagus ; Female ; Foreign Bodies ; Head ; Horses ; Humans* ; Jeollabuk-do ; Larva ; Membranes ; Ovum ; Pruritus ; Sensation ; Surgical Instruments ; Tail ; Thelazioidea* ; Uterus ; Vulva

An immunohistochemical study on the tissue localization of human chorionic gonadotropin (HCG), human placental lactogen (HPL), and human pregnancy specific bata-1 glycoprotein (SP-1) in the non-neoplastic (5 incomplete abortion, 5 ectopic pregnancy) and neoplastic (hydatidiform mole 3 cases, 5 ectopic pregnancy) and neoplastic (hydatidiform mole 3 cases, choriocarcinoma 2 cases) gestational status, and germ cell tumors of the ovary (dysgerminoma 2 cases, immature teratoma 1 case) and the testis (seminoma 2 cases, endodermal sinus tumor 1 case) by means of sensitive peroxidase-antiperoxidase method. The results were as follows. 1) Cytoplasmic HCG, HPL, and SP-1 were readily identified in syncytiotrophoblasts of chorionic villi of the incomplete abortion and ectopic pregnancy, as moderate to strong positive reactions. In the cytoplasm of the neoplastic syncytiotrophblasts of hydatidiform mole and choriocarcinoma, positive reactions were stronger than in non-neoplastic syncytiotrophblasts. but the cytotrophoblasts and villous stromal cells showed negative reactions to these hormones. 2) All of the tumor cells of dysgerminoma and immature teratoma of the ovary, and seminoma and endodermal sinus tumor of the testis showed negative reactions to HCG, HPL, and SP-1. According to these results, HCG, HPL, and SP-1 are specifically synthesized and secreted by normal or neoplastic syncytiotrophoblasts Malignant tumors originated from other cells or tissues than trophoblastic cells show elevated serum concentration of these hormones are only evident in the presence of syncytiotrophoblastic giant cells among the tumor cells.
Pregnancy ; Female ; Humans

BACKGROUNDS: The molecular genetic characteristcs of cis-AB blood group have shown that its allele had C, G, C and C at nucleotide positions (nps) 526, 703, 796 and 803, respectively. And all cis-AB analysed and reported molecular genetically in Korea and Japan had T at np 467 (leucine at amino acid position 156). We report a first case of cis-AB with C at np 467 (proline at amino acid position 156). METHODS: Genomic DNA was extracted from peripheral blood of cis-AB patient and amplified by DS1/DS2 and DS3/DS4 allele-specific primers. After PCR, we analysed nps 261, 467, 526, 646, 703, 796, and 803 by restriction digestion, autoradiography and automatic sequencing. RESULTS: PCR-RFLP with DS1/DS2 primers and restriction enzyme KpnI showed that cis-AB had an 0 allele. The results of genomic sequencing, autoradiography and restriction digestion showed that cis-AB allele at nps 467, 526, 646, 703, 796 and 803 had C, C, T, G, C and C, respectively. CONCLUSION: This cis-AB showed characteristic molecular genetic features at nps 526, 703, 796, and 803. And this is a first case of A(Pro) cis-AB with C at np 467. (Korean J Blood Transfusion 10(1): 13-19, 1999)
Alleles ; Autoradiography ; Blood Transfusion ; Cytosine* ; Digestion ; DNA ; Humans ; Japan ; Korea ; Molecular Biology* ; Polymerase Chain Reaction

For the morphological analysis of DMBA (9,10-diemethyl-1,2-benzanthracene) induced tumor, thirty Sprague-Dawley rats were received 0.1 ml of a 2% paraffin solution of DMBA into the knee joint cavity, which was repeated three times at an interval of 4 weeks. The induced tumor masses were removed at the 12th week after the first injection. Histological and histochemical examinations (H & E, PAS, alcian blue, Van Gieson, prussian blue, reticulin, PTAH stain) and enzyme histochemical examinations (acid phosphatase, alkaline phosphatase, alpha-naphthyl acetate esterase) were performed. The results were as follows: 1) By the 12th week after the first injection of DMBA, the tumor incidence rate was 20 percent. 2) On histological and histochemical examination, most of the induced tumor disclosed the features of the fibrous histiocytoma originating from mesenchymal cells, and the remains sweat gland adenoma and adenocarcinoma originating from epithelial cells. 3) On enzyme histochemical examination, most of the mesenchymal cell-derived tumor cells showed positive reactions for acid phosphatase and alpha-naphthyl acetate esterase, which were similar characteristic features of enzyme stains as shown in the component cells of fibrous histiocytoma.
Rats ; Animals ; Incidence ; Adenocarcinoma ; Adenoma