This experiment was conducted to assess the efficacy of typhoid vaccine newly produced by purifying Vi antigen of Salmonella typhi. With Karber method, LD50 of challenging organism (S. typhi ty2) was determined as 6.31 CFU/mouse, and then the organism was used for the study. With Probits method, ED50 of the vaccine was determined as 0.016 microgram / 0.5 ml / mouse. The ELISA titer (0.5097+/-0.0606) was 4 times in the group treated with high dose (0.25 microgram/0.5ml) as in control (0.1113+/-0.0110). Six major protein bands of 66, 55, 35, 33, 18, and 9 kd were detected in Western blot analysis with serum of a vaccine treated mouse, whereas only one weak band of about 35 kd was detected with serum of a control mouse. We concluded that typhoid vaccine produced by purifying Vi antigen of S. typhi very effectively prevent S. typhi infection in mice.
Animals ; Antibodies, Bacterial/immunology ; Antigens, Bacterial/*immunology/*isolation&purification ; Blotting, Western ; Enzyme-Linked Immunosorbent Assay ; Lethal Dose 50 ; Logistic Models ; Male ; Mice ; Mice, Inbred BALB C ; Polysaccharides, Bacterial/*immunology/*isolation&purification ; Salmonella typhi/chemistry/*immunology ; Typhoid Fever/immunology/prevention&control ; Typhoid-Paratyphoid Vaccines/administration & dosage/*immunology/*isolation&purification