1.Extraintestinal salmonellosis.
Sang Gyung KIM ; Young Sik CHOI ; Woong Soo LEE ; Tae Yeal CHOI ; Choon Won KIM ; Ho Won KIM
Korean Journal of Clinical Pathology 1991;11(3):627-631
No abstract available.
Salmonella Infections*
2.Salmonellosis.
Korean Journal of Clinical Microbiology 2001;4(1):5-10
No abstract available.
Salmonella Infections*
3.Rapid confirmation of five non-typhoidal Salmonella (NTS) serotypes using a low-cost portable-DIY OpenPCR© thermocycler
Huda Alkatib ; Carlos Silvester ; Dinesh Balachandra ; Freddy Franklin Joseph ; Sasidharan Sreenivasan ; Kia Kien Phua
Malaysian Journal of Microbiology 2021;17(2):113-120
Aims:
OpenPCR is a low cost yet accurate thermocycler which can be self-built. The aim of the study is to highlight a
low-cost alternative method for rapid confirmation of five predominant non-typhoidal Salmonella (NTS) serotypes using a
multiplex PCR on a portable-DIY OpenPCR© thermocycler.
Methodology and results:
Eight multiplex polymerase chain reaction (mPCR) samples containing genomic DNA of S.
Agona selectively placed on the wells of the conventional PCR and OpenPCR© thermocyclers showed uniform heating in
both thermocyclers. The limit of detection was similar for both thermocyclers for all five serotypes. The limit of detection
for S. Typhimurium, S. Agona and S. Weltevreden was 10 pg/µL whereas the limit of detection for S. Enteriditis and S.
Heidelberg was 1 pg/µL and 100 pg/µL, respectively. This assay incorporated a panel of unique genes; STM4495,
SEN1392, SeHa-C4893, SeAg-B1096 and SENTW-3241 which were previously identified to be specific for S.
Typhimurium, S. Enteritidis, S. Heidelberg, S. Agona, and S. Weltevreden, respectively, as well as the pan-Salmonella
gene invA as internal control (IC) and pan-bacteria gene 16S rRNA to serve as amplification control (AC). The analytical
specificity of the mPCR assay was found to be 100% for all five NTS using OpenPCR© thermocyclers.
Conclusion, significance and impact of study
The feasibility and low cost of the OpenPCR© thermocycler makes this
device an ideal alternative for mPCR assay for rapid confirmation of NTS serotypes.
Salmonella Infections
4.Serogroups and antibiogram of Salmonella isolates from dairy cattle in Nkonkobe District, South Africa
Benson Chuck Iweriebor ; Olivia Sochi Egbule ; Aboi Igwaran ; Larry Chikwulu Obi
Malaysian Journal of Microbiology 2021;17(4):452-458
Aims:
The use of antimicrobial agent for treatment or growth promotion has added burden to treat infection diseases
caused by pathogenic bacteria as they can acquire resistance. Salmonella is one of the major zoonotic bacterial
pathogens that acts as a potential reservoir of antimicrobial resistance elements. In this study, the presence of
Salmonella serotypes and the antibiogram patterns of the isolates from fecal samples of healthy cows in some selected
localities in Eastern Cape, South Africa were studied.
Methodology and results:
Two hundred fecal samples were collected from healthy adult cows, of which 180
presumptive Salmonella isolates were recovered by conventional method. The isolates were identified using specific
primer sets that are capable of detecting Salmonella spp. as well as delineating them into serogroups A, B, C1, C2, and
D. Thereafter, antimicrobial susceptibility patterns of the identified isolates were determined by disk diffusion method
against a panel of 12 antibiotics. From the molecular analysis of the isolates, 108 isolates were identified as Salmonella
spp. and the confirmed isolates were further delineated into serogroup and the prevalence of the serogroups detected
were 20%, 18%, 2%, 20% and 40% for serogroup A, B, C1, C2 and D respectively. Extremely high levels of antibiotic
resistances were observed among the study isolates, while serogroup D was the most prevalent serogroup among the
study isolates.
Conclusion, significance and impact of study
In conclusion, dairy cows could be considered as major reservoirs of
antibiotic resistant Salmonella spp. that could be transmitted to humans via the food chain. This poses a significant
public health risk especially to people living around the farms as well as those who consume poorly cooked meat and
those who deal on raw cow meat.
Salmonella Infections, Animal
5.Loop-mediated isothermal amplification assay for the detection of Salmonella spp. in pig feces.
Yong Kwan KIM ; Ha Young KIM ; Albert Byungyun JEON ; Myoung Heon LEE ; You Chan BAE ; Jae Won BYUN
Korean Journal of Veterinary Research 2014;54(2):113-115
Salmonella are causative agents of gastroenteritis and systemic disease in animals. The invA gene was selected as a target sequence of loop-mediated isothermal amplification (LAMP) assay for diagnosis of Salmonella infection. The detection limits for broth dilution, spiked feces and enrichment were 10(4), 10(5) and 10(2) CFUs/mL, respectively. The LAMP assay developed in the present study may be a reliable method for detection of Salmonella spp. in pig feces.
Animals
;
Diagnosis
;
Feces*
;
Gastroenteritis
;
Limit of Detection
;
Salmonella Infections
;
Salmonella*
6.First Report of Salmonella Serotype Tilene Infection in Korea.
Su Jin CHAE ; Young Sun YUN ; Cheon Kwon YOO ; Gyung Tae CHUNG ; Deog Yong LEE
Annals of Clinical Microbiology 2016;19(1):24-27
Salmonellosis is a common food- and water-borne disease and is also a major zoonosis. Currently, the isolation of rare Salmonella serotypes is increasing every year in Korea. Among them, the Salmonella serotype Tilene was first isolated from two people who visited a hospital located in Andong-si in 2013. Clinical symptoms were weak or non-existent. There was no clear epidemiological connection between the two cases. However, it was assumed that both were independently exposed to a single infectious agent. Perhaps due to their geographical proximity, molecular epidemiological analysis showed the same result between the isolated strains. This serotype has increasingly reported an association with hedgehogs. Recently, the importation of exotic animals, including hedgehogs, as pets has been gradually increasing. Thus, it is recommended that high-risk groups avoid contact with exotic pets.
Animals
;
Gyeongsangbuk-do
;
Hedgehogs
;
Korea*
;
Salmonella Infections
;
Salmonella*
7.Rupture of Popliteal Arterial Aneurysm due to Salmonella Infection.
Dong Hun KIM ; Hyung Woo OH ; Dong Hyun KIM ; Joo Nam BYUN
Journal of the Korean Radiological Society 2006;55(3):235-238
We report here on a case of popliteal aneurysm and rupture that occurred over a 10-day period and this was all secondary to salmonella infection. Computed tomography (CT) angiography of the extremity that was performed before and after aneurysmal rupture showed the aneurysm's rapid evolution to rupture over a short period of time. We also review the pathogenesis, clinical presentation, diagnostic approach and management of salmonella aneurysms.
Aneurysm*
;
Angiography
;
Extremities
;
Popliteal Artery
;
Rupture*
;
Salmonella Infections*
;
Salmonella*
8.Rupture of Popliteal Arterial Aneurysm due to Salmonella Infection.
Dong Hun KIM ; Hyung Woo OH ; Dong Hyun KIM ; Joo Nam BYUN
Journal of the Korean Radiological Society 2006;55(3):235-238
We report here on a case of popliteal aneurysm and rupture that occurred over a 10-day period and this was all secondary to salmonella infection. Computed tomography (CT) angiography of the extremity that was performed before and after aneurysmal rupture showed the aneurysm's rapid evolution to rupture over a short period of time. We also review the pathogenesis, clinical presentation, diagnostic approach and management of salmonella aneurysms.
Aneurysm*
;
Angiography
;
Extremities
;
Popliteal Artery
;
Rupture*
;
Salmonella Infections*
;
Salmonella*
9.Salmonella enteritis: A Rare Cause of Adult Intussusception.
Toan PHAM ; Domenic LA PAGLIA ; Meron PITCHER
Annals of Coloproctology 2017;33(5):201-203
Intussusception is a relatively rare condition in the adult population and is commonly secondary to a malignant process. Eight to twenty percent of cases of adult intussusception are thought to be idiopathic. In children, infection has been proven to precipitate intussusception in the absence of any other cause. We present a rare case of intussusception in a healthy adult patient secondary to salmonella infection and discuss infection as a potential explanation for a proportion of the cases of adult intussusception that are thought to be idiopathic. We recommend testing for infective causes of intussusception in adults when more common causes, such as malignancy, have been excluded.
Adult*
;
Child
;
Enteritis*
;
Humans
;
Intussusception*
;
Salmonella Infections
;
Salmonella*
10.Salmonella enteritis: A Rare Cause of Adult Intussusception.
Toan PHAM ; Domenic LA PAGLIA ; Meron PITCHER
Annals of Coloproctology 2017;33(5):201-203
Intussusception is a relatively rare condition in the adult population and is commonly secondary to a malignant process. Eight to twenty percent of cases of adult intussusception are thought to be idiopathic. In children, infection has been proven to precipitate intussusception in the absence of any other cause. We present a rare case of intussusception in a healthy adult patient secondary to salmonella infection and discuss infection as a potential explanation for a proportion of the cases of adult intussusception that are thought to be idiopathic. We recommend testing for infective causes of intussusception in adults when more common causes, such as malignancy, have been excluded.
Adult*
;
Child
;
Enteritis*
;
Humans
;
Intussusception*
;
Salmonella Infections
;
Salmonella*