No abstract available.
Animal ; Isomerism ; Saliva/secretion* ; Sodium-Hydrogen Antiporter/secretion ; Sodium-Hydrogen Antiporter/physiology* ; Sodium-Hydrogen Antiporter/chemistry*

No abstract available.
Animal ; Isomerism ; Saliva/secretion* ; Sodium-Hydrogen Antiporter/secretion ; Sodium-Hydrogen Antiporter/physiology* ; Sodium-Hydrogen Antiporter/chemistry*

OBJECTIVETo investigate the effect of parasympathectomy on secretion of submandibular glands and the feasibility of treatment for xerostomia in rats.

METHODSTwenty Sprague-Dawley male rats weighing 200 - 300 g were randomly divided into the experimental group (n = 12), in which the right chorda-lingual nerve was cut, and the control group (n = 8). The secretion of submandibular gland was measured for 5 min by Schirmer test for both groups.

RESULTSThe stimulated saliva flow rate decreased on 1st, 12th and 24th week after denervation in the right operated submandibular glands (P < 0.05). No difference in secretion was found between the left non-operated glands and the control group. Comparing with the left non-operated gland and the control gland, the saliva flow rate at rest in the right operated submandibular gland increased on the 1st, 12th and 24th week (P < 0.05).

CONCLUSIONSAfter parasympathectomy of rat submandibular glands, the saliva flow rate at rest increased in the denervated gland, which suggests that parasympathectomy of submandibular gland might be used as a therapy for xerostomia.

Animals ; Male ; Parasympathectomy ; methods ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Saliva ; secretion ; Salivation ; Submandibular Gland ; secretion ; Xerostomia ; therapy

OBJECTIVETo analyze the effects of a Qigong program on various parameters of unstimulated saliva, including volume, pH and secretory immunoglobulin A (S-IgA) level.

METHODSTwice a day from the beginning of Fall 2005, twenty-three healthy volunteers aged 22-24 did special Qigong exercises and massage of acupuncture points which stimulated the energy cycle and increased body water energy. The unstimulated saliva volume and pH were recorded every week in Spring (April, May, June) 2005 before the volunteers started to learn and exercise Qigong, and after Qigong intervention in Spring (April, May, June) 2006. During the period of study, saliva was collected in the same location and on similar dates at the Dental Faculty of Tehran University of Medical Sciences. The S-IgA levels of the last samples of the last week of Spring 2005 and 2006 were measured.

RESULTSThe unstimulated saliva volume after Qigong exercises (2.94 + or - 0.20 mL/5min) was significantly higher as compared to the pre-Qigong phase (1.65 + or -0.102 mL/5min, P<0.05). The S-IgA level was 105.45 + or - 9.41 mg/mL before doing Qigong exercises, and 156.23 + or - 88.56 mg/mL after doing Qigong exercises, and a statistically significant difference was seen between the two measurements (P=0.005). The change in pH was not statistically significant.

CONCLUSIONSThe application of Qigong is beneficial for increasing salivary volume and other parameters. Moreover, the results suggest that Qigong may be a useful medication for patients with salivary hyposecretion. Further research is recommended in examining the long-term effects of Qigong on improving salivary volume and other parameters in individuals with salivary hyposecretion.

Breathing Exercises ; Female ; Humans ; Hydrogen-Ion Concentration ; Immunoglobulin A, Secretory ; analysis ; Male ; Saliva ; immunology ; secretion ; Young Adult

OBJECTIVETo observe the effect of capsaicin on the secretion of the transferred gland in "latent period".

METHODSForty-two patients (45 sides of eye), who received the microvascular autologous submandibular gland transfer in our hospital, were treated with two different types of capsaicin stimulations. The secretion function of the transferred glands was evaluated by Schirmer's test. The effect of capsaicin and its time-variation regularity were observed. In the meantime, the results of capsaicin were compared with some other stimulation methods, such as acid, chewing and physiotherapy with infrared rays.

RESULTSThe capsaicin gargle, capsaicin cream and physiotherapy could all promote the secretion of the transferred gland, while the effects of the capsaicin stimulation were more obvious. The other two stimulations (acid and chewing) had no effect on the secretion of the transferred gland.

CONCLUSIONSThe capsaicin stimulation can be taken as one of the effective means to promote the secretion of the transferred glands in "latent period" and to prevent the obstruction of the ducts. Because of its convenience and blandness, the capsaicin cream is the first choice of the clinical application options for the transferred submandibular gland in "latent period".

Adolescent ; Adult ; Capsaicin ; pharmacology ; Child ; Female ; Humans ; Keratoconjunctivitis Sicca ; surgery ; Male ; Middle Aged ; Saliva ; secretion ; Submandibular Gland ; secretion ; transplantation ; Transplantation, Autologous ; Young Adult

To analyze the specific mechanism of Jinxueyuan granules, the relationship between the Jinxueyuan granules increased the saliva secretion of xerostomia model SD rats and excitement of receptors were studied in this experiment. In the study, three groups of xerostomia model rats were successfully established by using M-receptor blockers-4-diphenyl-acetoxy-N-methyl-piperidine (4-DAMP) and atropine, or adrenergic receptor blocker phentolamine; after the modeling, the medicine Jinxueyuan granules were gavaged. According to the clinical dose of Jinxueyuan granules and SD rats body surface area, the rats in atropine group were divided three dose groups respectively, namely low, medium and high dose of Jinxueyuan granules groups. The 4-DAMP group and phentolamine group were gavaged medium dose of Jinxueyuan granules. And the amount of salivary secretion for 150 minutes in all groups continuously were measured, and the effect of Jinxueyuan granules increased salivation and the relationship between characteristics and the receptors were observed; and submandibular gland tissue of the rats was isolated, then the effect of Jinxueyuan Granules for expression of the water channel protein aquaporin-5 (AQP5) in submandibular gland cells was analyzed by the Western blot technology. It was found that the saliva secretion of Jinxueyuan Granules groups was increased significantly, and compared with the saline control group, phentolamine group, 4-DAMP group and atropine group, difference was significant, P < 0.05. There was no significant difference between the low-dose of Jinxueyuan granules group and the saline group, but the medium dose of Jinxueyuan granules group had a significant difference, compared with the saline group (P < 0.05). In the time distribution of increasing saliva secretion, there was a significant difference between the saline and Jinxueyuan granules group in the saliva secretion (P < 0.05). After administration of Jinxueyuan granules, the expression of AQP5 protein in the submandibular gland cells expressing of treatment groups was increased, and compared with the blocker groups, there was a significant difference, P < 0.05. Except the atropine group, there was no significant difference in Jinxueyuan granules relieving the inhibition induced by blocks in phentolamine group and 4-DAMP group, compared with the saline group. Compared the AQP5 expression in three blockers groups, there was no significant difference in the efficacy of Jinxueyuan granules between phentolamine group and 4-DAMP group; but there was a significant difference between the atropine group and other groups (P < 0.05). Therefore, it was considered that the mechanism of Jinxueyuan granules increasing saliva secretion (effectiveness of nourishing Yin and generating body fluid ) possibly through the pathway mediated by muscarinic M receptor, especially M3 receptor, or adrenergic receptor, and increased expression of salivary gland AQP5 membrane, and then stimulate saliva production.
Animals ; Disease Models, Animal ; Drugs, Chinese Herbal ; administration & dosage ; Humans ; Male ; Rats ; Saliva ; secretion ; Salivary Glands ; drug effects ; secretion ; Xerostomia ; drug therapy ; metabolism

OBJECTIVETo observe the effect of bilateral parotid gland atrophy on the whole saliva flow rate and the growth of main oral pathogens in different sites of oral cavity.

METHODSTen healthy miniature pigs were divided into two groups. The parotid glands of test group (n = 5) were bilaterally ablated by methyl violet. Another healthy five miniature pigs served as the control group. Whole saliva was collected and the whole saliva flow rate detected in both groups at 12 and 24 months respectively after parotid atrophy. The total numbers of oral main pathogens in the first molar, cuspid sub-gingival bacteria plaque and whole saliva were also detected.

RESULTSThe whole saliva flow rate was significantly decreased at both 12 and 24 months respectively after atrophy of bilateral parotid gland in miniature pig. Pathogens including Streptococcus mutans, Porphyromonas gingivalis and Fusobacterium nucleatum in different sites oral cavity were increased after bilateral parotid gland atrophy.

CONCLUSIONSBilateral ablation of the parotid glands led to a significant decrease of whole saliva flow rate. The total numbers of main oral pathogens were increased in different sites of oral cavity.

Animals ; Atrophy ; Disease Models, Animal ; Mouth ; microbiology ; Parotid Gland ; pathology ; Random Allocation ; Saliva ; secretion ; Swine ; Swine, Miniature

OBJECTIVETo evaluate the thickness and viscoelasticity of whole saliva (WS), parotid saliva (PS) and submandibular/sublingual gland saliva (SMSLS) film adsorption on the hydroxyapatite (HA) surface.

METHODSUltra-thin layer of HA nanocrystals was coated on the dissipation TiO(2) sensor of gold quartz crystal microbalance using electrophoretic deposition technique. The thickness of the HA layer was measured by the ellipsometer, and element analysis was conducted using X-ray photoelectron spectroscopy. Atomic force microscopy and scanning electron microscope were used to observe its morphology. The in-situ adsorption thickness, the shear elastic modulus and the shear viscosity of salivary layers (WS, PS and SMSLS) on HA surfaces were investigated. The statistical data were analysed by an one-way ANOVA analysis followed by a SNK-q test.

RESULTSThe results show that the HA layer was a plate-like morphology with 1.53 ± 0.12 in Ca/P molar ratio, (19.1 ± 0.9) nm in the thickness and (6.5 ± 1.6) nm in the roughness. The thickness of salivary film was SMSLS [(21.84 ± 1.25) nm] > WS[(17.91 ± 1.35) nm] > PS [(14.30 ± 1.03 nm) (P < 0.05). The shear elastic modulus of salivary film was PS [(0.61 ± 0.01) MPa] > SMSLS [(0.31 ± 0.09) MPa] and WS [(0.25 ± 0.03) MPa] (P < 0.05). The trend of the shear viscosity was opposite to one of thickness.

CONCLUSIONSThe characteristics of saliva adsorption on HA surface suggest that the thicker, softer and more hydrated properties for the SMSLS and WS films are likely to afford a stronger lubrication to protect oral surfaces from wear and dehydration. The viscoelasticity of the PS film is probably related to the retention covering the oral cavity.

Adsorption ; Adult ; Durapatite ; chemistry ; Female ; Humans ; Male ; Microscopy, Atomic Force ; Parotid Gland ; secretion ; Photoelectron Spectroscopy ; Quartz Crystal Microbalance Techniques ; Saliva ; chemistry ; Sublingual Gland ; secretion ; Submandibular Gland ; secretion ; Surface Properties ; Viscosity ; Young Adult

OBJECTIVETo study the variations of protein concentration in saliva stimulated and its effect on clinical diagnosis.

METHODSThe saliva from 33 normal controls and 73 patients with Sjögren syndrome (SS) who were stimulated with acid and not were collected. The concentration of beta 2 microglobulin (beta 2-mG), secretory immunoglobulin A (SIgA), and pH were measured by Radioimmunoassay, Rate Nephelometry and pH Detection Paper, respectively. SPSS 10.0 was used to determine the mean statistical differences among these groups.

RESULTSIn patients with SS, the concentration of beta 2-mG in saliva stimulated with Vc was significantly lower compared with that in saliva not stimulated (P < 0.01); In saliva stimulated with Vc, the concentration of beta 2-mG in patients with SS was higher than that in normal controls (P < 0.05). In normal controls, compared with that in saliva not stimulated, flow rate in saliva stimulated with 3% acetic acid and Vc was significantly higher (P < 0.01) and pH, concentration of beta 2-mG and SIgA were significantly lower (P < 0.01 and P < 0.05 respectively); there was a significant difference of flow rate, beta 2-mG, SIgA and pH in saliva between the subjects stimulated with 3% acetic acid and with Vc (P < 0.01).

CONCLUSIONSThe reason for the decrease of protein concentration in saliva stimulated may be the increase of flow rate caused by the decrease of pH or the decrease of pH itself. Protein detection of saliva stimulated in patients with SS is helpful in diagnosis, but the criterion is different between the saliva stimulated and not stimulated.

Adult ; Female ; Humans ; Hydrogen-Ion Concentration ; Immunoglobulin A, Secretory ; metabolism ; Male ; Saliva ; metabolism ; secretion ; Salivary Proteins and Peptides ; metabolism ; Sjogren's Syndrome ; diagnosis ; metabolism ; beta 2-Microglobulin ; metabolism

OBJECTIVETo observe the alterations of saliva nitrate and nitrite level in patients with oral candidiasis.

METHODSParotid saliva and whole saliva were collected from 33 patients and 34 healthy volunteers. Concentrations of nitrate and nitrite in saliva were determined by high-performance liquid chromatography. Follow-up observation was performed on 10 patients after treatment. The data were statistically analyzed with independent-samples t test or paired-samples t test at alpha = 0.05.

RESULTSThere was significant increase of the concentrations and secretion rate of parotid saliva nitrate in patient group as compared with controls: (49.70 +/- 0.50) vs (21.51 +/- 0.60) mg/L (t = 2.692, P = 0.009) and (27.71 +/- 0.50) vs (12.55 +/- 0.60) microg/min (t = 2.554, P = 0.013), respectively. Significantly increased concentrations and secretion rate of nitrate and nitrite [nitrate: (6.46 +/- 0.94) vs (1.11 +/- 0.70) mg/L (t = 3.792, P = 0.000); nitrite: (8.48 +/- 0.58) vs (3.39 +/- 0.53) mg/L (t = 2.888, P = 0.005); nitrate secretion rate: (10.57 +/- 0.91) vs (2.10 +/- 0.74) microg/min (t = 3.464, P= 0.001); nitrite secretion rate: (13.91 +/- 0.55) vs (6.42 +/- 0.58) microg/min (t = 2.397, P = 0.020)] were revealed in whole saliva of patients group. Significantly decreased nitrate and nitrite levels were also observed in patients after treatment, especially the changes of parotid saliva nitrate secretion rate [(37.50 +/- 0.50) vs (14.34 +/- 0.64) microg/min (t = 3.142, P = 0.012)], whole saliva nitrate [(14.29 +/- 1.01) vs (2.59 +/- 1.03) mg/L (t = 3.475, P = 0.007)] and whole saliva nitrate secretion rate [(25.97 +/- 0.93) vs (4.12 +/- 1.00) microg/min (t = 3.922, P = 0.003)].

CONCLUSIONThe present study revealed the significant increase of salivary nitrate and nitrite level in patients with oral candidiasis is considered to be associated with the host defense reaction.

Adult ; Aged ; Candidiasis, Oral ; metabolism ; Case-Control Studies ; Female ; Humans ; Male ; Middle Aged ; Nitrates ; metabolism ; Nitrites ; metabolism ; Saliva ; secretion ; Young Adult