1.Association of Polymorphisms within the Serotonin Receptor Genes 5-HTR1A, 5-HTR1B, 5-HTR2A and 5-HTR2C and Migraine Susceptibility in a Turkish Population.
Yavuz YÜCEL ; Salih COŞKUN ; Beyhan CENGIZ ; Hasan H ÖZDEMIR ; Ertuğrul UZAR ; Abdullah ÇIM ; M Akif CAMKURT ; M Ufuk ALUCLU
Clinical Psychopharmacology and Neuroscience 2016;14(3):250-255
OBJECTIVE: Migraine, a highly prevelant headache disorder, is regarded as a polygenic multifactorial disease. Serotonin (5-HT) and their respective receptors have been implicated in the patogenesis. METHODS: We investigated the 5-HT1A, 5-HT1B, 5-HT2A, and 5-HT2C receptor gene polymorphisms and their association with migraine in Turkish patients. The rs6295, rs1300060, rs1228814, rs6311, rs6313, rs6314, rs6318, rs3813929 (−759C/T) and rs518147 polymorphisms were analyzed in 135 patients with migraine and 139 healthy subjects, using a BioMark 96.96 dynamic array system. RESULTS: We found no difference in the frequency of the analyzed eight out of nine polymorpisms between migraine and control groups. However, a significant association was found between the rs3813929 polymorphism in the promoter region of 5-HTR2C gene and migraine. Also, the allele of rs3813929 was more common in the migraine group. CONCLUSION: This result suggests that the 5-HTR2C rs3813929 polymorphism can be a genetic risk factor for migraine in a Turkish population.
Alleles
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Genetic Association Studies
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Headache
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Headache Disorders
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Healthy Volunteers
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Humans
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Migraine Disorders*
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Polymorphism, Single Nucleotide
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Promoter Regions, Genetic
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Receptor, Serotonin, 5-HT2C
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Risk Factors
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Serotonin*
2.Comparison of total plasma lysophosphatidic acid and serum CA-125 as a tumor marker in the diagnosis and follow-up of patients with epithelial ovarian cancer.
Tugan BESE ; Merve BARBAROS ; Elif BAYKARA ; Onur GURALP ; Salih CENGIZ ; Fuat DEMIRKIRAN ; Cevdet SANIOGLU ; Macit ARVAS
Journal of Gynecologic Oncology 2010;21(4):248-254
OBJECTIVE: To evaluate the role of lysophosphatidic acid (LPA) as a tumor marker in diagnosis and follow-up of patients with epithelial ovarian cancer. METHODS: Eighty-seven epithelial ovarian cancer patients, 74 benign ovarian tumor patients, and 50 healthy women were enrolled in the study. Twenty-nine of 87 epithelial ovarian cancer patients were followed up for 6 cycles of paclitaxel-carboplatin chemotherapy. CA-125 and total plasma LPA levels were measured preoperatively and before each chemotherapy cycle. RESULTS: Preoperative total plasma LPA and serum CA-125 levels were significantly higher in patients with epithelial ovarian cancer compared to patients with benign ovarian tumors and healthy women. Cut-off value for LPA was determined as 1.3 micromol/L and sensitivity, specificity, positive predictive value and negative predictive value were 95%, 92%, 95% and 92%, respectively. Mean total plasma LPA level of 29 patients who received chemotherapy was 7.21+/-6.63 micromol/L preoperatively and 6.84+/-6.34 micromol/L, 6.34+/-5.92 micromol/L, 6.14+/-5.79 micromol/L, 5.86+/-5.68 micromol/L, 5.23+/-5.11 micromol/L and 5.21+/-5.32 micromol/L in measurements held just before the 1st, 2nd, 3rd, 4th, 5th and 6th chemotherapy cycles, respectively (ANOVA, p=0.832). Total plasma LPA levels decreased slightly with chemotherapy administration and there was a weak negative correlation (Spearman, rs=-0.151, p=0.034), compared to a significant negative correlation in CA-125 (Spearman, rs=-0.596, p<0.001). CONCLUSION: LPA is a better biomarker for diagnosis of epithelial ovarian cancer compared to CA-125. However, measurement of total plasma LPA levels during chemotherapy administration have no superiority to the serum CA-125 levels.
Female
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Follow-Up Studies
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Humans
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Lysophospholipids
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Neoplasms, Glandular and Epithelial
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Ovarian Neoplasms
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Plasma
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Sensitivity and Specificity