Objective To establish and optimize the method of isolation and cultivation of rat bone marrow mesenchymal stem cells(MSCs),and to investigate the condition and capability of hepatic differentiation of MSCs.(Methods) Rat bone marrow cells were collected from rats of 5～6 week and mononeuclear cells were isolated by Percoll density gradient centrifugation.MSCs were cultivated and purified by adherence method.Morphology,RT-PCR and immunocytochemistry were used to identify the role of substrates,the kinds of the cytokines and concentrations of cytokines on the hepatic differentiation potential.Results The quality and quantity of the isolated bone marrow mononuclear cells reached the peak by using 57% Percoll density gradient centrifugation.Discarding the suspending cells after 24h incubation and subculturing the cells with low seeding density were helpful for acquiring MSCs with improved reproductive capability and active function.MSCs could be successfully induced under HGF/FGF-4 induction on the substrate of(10 ?g/mL) fibronectin.MSCs exhibited round in shape after differentiation,instead of fibroblast-like morphology before.Albumin mRNA and protein were positively expressed in MSCs,withoutdetection of alpha-fetoprotein(AFP).Conclusion The optimization of rats' age,the density of Percoll,the methods of cell cultivation are conducing to obtaining a pure population of MSCs with good differential activity.MSCs are inclined to differentiate into mature hepatocyte-like cells under the induction of HGF/FGF-4.

The antidromic C waves elicited by stimulating the entry zone of the dorsal root were recorded in spinal toads. Influences if conditioning stimuli in 60 T from sciatic nerve on antidromic C waves were observed. The conditioning stimuli produced marked increace in the excitability of the C-afferent terminals. The increased excitability possessed two phases. The results indicat that periphiral primary afferent A and C-fibres can produce presynaptic inhibition in C-fibre terminals.

Acute leukemia is the most common hematological malignancies.Based on the development of chemotherapy drugs and the improvement of chemotherapy regimens,the remission rate after induction chemotherapy increases obviously.But leukemia patients can not live for a long time mainly because of the ease relapse,so how to extend remission after treatment become the key to solve the problem.People are paying more and more attention on the cellular immunotherapy since it playes major roles in eradicating minimal residual disease of leukemia,reducing leukemia relapse and improving leukemia patients overall survival.This article reviews the application advances in research on this subject.

Fecal microbiota transplantation(FMT)is a method to recover the gut microbiota and treat the intestinal or non-intestinal diseases through transplanting the fecal liquid from healthy population into patients' gastrointestinal tract.There are limited data about the FMT in children.The clinical practice needs professional workgroups,strict indications,normalized and precise procedures.

Objective To observe the clinical efficacy of moxibustion plus Cang Gui Tan Xue (dark turtle seeking hole) needling method in treating cervical spondylosis of nerve root type. Method Sixty patients with cervical spondylosis of nerve root type were randomized into a treatment group and a control group, 30 in each group. The treatment group was intervened by moxibustion plus Cang Gui Tan Xue needling method, and the control group was by simple needling method. The symptom and sign score was observed before and after 2-course treatment, and the clinical efficacy was compared. Result The total effective rate was 90.0%in the treatment group versus 83.3%in the control group, and the difference was statistically significant (P<0.05). After intervention, the symptom and sign scores were significantly changed in both groups (P<0.05). There was a significant difference in comparing the symptom and sign score between the two groups after intervention (P<0.05). Conclusion Moxibustion plus Cang Gui Tan Xue needling method is effective in treating cervical spondylosis of nerve root type.

Objective:To analyze the profound influence of the Dictionary of Occupation on the career development of medical rep-resentatives in our country. Methods:The relationship of the Dictionary of Occupation and the career development of medical represent-atives was discussed, and then the influence of including the dictionary on medical representatives was analyzed. Results and Conclu-sion:The Dictionary of Occupation puts forward higher requests to the code of conduct, professional ability and professional ethics of medical representatives, which plays important roles in the career development of medical representatives. And as a professional stand-ard with clear definition and description, the Dictionary of Occupation also leads to the direction of sustainable and healthy development of medical representatives.

Objective To systematically evaluate the curative effect and safety of treating winter diseases in summer acupoint application therapy for asthma in children.Methods The domestic and international databases were retrieved till Feb 2017.The randomized controlled trials about treatment of treating winter diseases in summer acupoint application therapy for children with asthma were identified.The methodology quality of included trials was assessed.Results A total of 3 RCT involving 146 patients were included.The compositions of Chinese medicines and acupuncture points used were quite diverse,which inhibited us to conduct meta-analysis.The methodological quality of included RCT was generally lower.The results showed that treating winter diseases in summer acupoint application therapy might have advantage in reducing asthma acute attack times and improving clinical symptoms and respiratory function.Conclusion treating winter diseases in summer acupoint sticking therapy for asthma in children may have some advantages,however,due to the lack of a number of trials and poor methodology quality,the claimed benefits of treating winter diseases in summer acupoint sticking therapy for for children with asthma are inconclusive;more rigorous studies are warranted to support clinical practice.

Objective: To study alcohol related attention damage and factors having influence on it. Method: 39 inpatients with alcohol dependence were examined with Continuously Performance Test (CPT) before and after 6 weeks treatment. 45 normal staffs were collected as control. Results: The index of CPT in patient group at baseline was higher than that of normal control. After 6 weeks treatment, the above index declined but was still higher than control. The CPT results before treatment were related to plasma level of alcohol when admitted, age and history of alcohol use. Conclusion: Alcohol dose damage to the attention of patients in dependence.

Objective To find a sensitive index and to investigate the working memory impairment of patients with amnestic mild cognitive impairment.Methods Fifteen patients with amnestic mild cognitive impairment,15 healthy matching aging controls were performed a matching-to-sample task while event-related potential(ERP),reaction time and correct rate were recorded.Subjects were required to press a button in the match condition and another button in the conflict condition.Results In the matching condition,there was no difference between the two groups in distribution(parietal lobe),peak latency and amplitude of P300(F_ 1,28 =1.0324,P=0.3183;F_ 2,42 =0.543,P=0.585).In the conflict condition,the distribution of N270 was fore head,and its latency of patient group were more delayed than the aging group(F_ 1,28 =25.3264,P=0.000),but its amplitude showed no significant changes(F_ 1,28 =0.507,P=0.482).The result of brain mapping showed same change.Conclusion The N270 component is more sensitive than P300 to reflect the central executive function impairment of working memory in patients with amnestic mild cognitive impairment.

Objective To study the protective effect and molecular mechanism of trypsin inhibitor on reperfusion injury after cerebral ischemia. Methods The model of ischemia for 1h and repufusion for 24h of rat cerebrum was set by ligating MCAO described by zea-longa. 24 male rats were divided randomly into the sham operation group, the control group and trypsin inhibitor group. The presence of neurological function deficit was measured by Zea-Longa method, and the immunohistochemical staining and TUNEL were used to detect p53 protein expression and cell apoptosis in the brain tissues respectively. Results The score of neurological function deficit was zero in the sham operation group, 2 8?1 0 in the control group and 1 3?0 7 in trypsin inhibitor group. There were 12 3?2 5 p53 immunostaining positive cells in the control group and 5 5?1 3 in trypsin inhibitor group. The number of apoptotic cells was zero in the sham operation group, 7 6?1 0 in the control group, and 3 5?0 9 in trypsin inhibitor group. There was a significant difference in all above observing indices between the control group and trypsin inhibitor group(P