Acute leukemia is the most common hematological malignancies.Based on the development of chemotherapy drugs and the improvement of chemotherapy regimens,the remission rate after induction chemotherapy increases obviously.But leukemia patients can not live for a long time mainly because of the ease relapse,so how to extend remission after treatment become the key to solve the problem.People are paying more and more attention on the cellular immunotherapy since it playes major roles in eradicating minimal residual disease of leukemia,reducing leukemia relapse and improving leukemia patients overall survival.This article reviews the application advances in research on this subject.

The antidromic C waves elicited by stimulating the entry zone of the dorsal root were recorded in spinal toads. Influences if conditioning stimuli in 60 T from sciatic nerve on antidromic C waves were observed. The conditioning stimuli produced marked increace in the excitability of the C-afferent terminals. The increased excitability possessed two phases. The results indicat that periphiral primary afferent A and C-fibres can produce presynaptic inhibition in C-fibre terminals.

Objective To establish and optimize the method of isolation and cultivation of rat bone marrow mesenchymal stem cells(MSCs),and to investigate the condition and capability of hepatic differentiation of MSCs.(Methods) Rat bone marrow cells were collected from rats of 5～6 week and mononeuclear cells were isolated by Percoll density gradient centrifugation.MSCs were cultivated and purified by adherence method.Morphology,RT-PCR and immunocytochemistry were used to identify the role of substrates,the kinds of the cytokines and concentrations of cytokines on the hepatic differentiation potential.Results The quality and quantity of the isolated bone marrow mononuclear cells reached the peak by using 57% Percoll density gradient centrifugation.Discarding the suspending cells after 24h incubation and subculturing the cells with low seeding density were helpful for acquiring MSCs with improved reproductive capability and active function.MSCs could be successfully induced under HGF/FGF-4 induction on the substrate of(10 ?g/mL) fibronectin.MSCs exhibited round in shape after differentiation,instead of fibroblast-like morphology before.Albumin mRNA and protein were positively expressed in MSCs,withoutdetection of alpha-fetoprotein(AFP).Conclusion The optimization of rats' age,the density of Percoll,the methods of cell cultivation are conducing to obtaining a pure population of MSCs with good differential activity.MSCs are inclined to differentiate into mature hepatocyte-like cells under the induction of HGF/FGF-4.

Objective:To analyze the profound influence of the Dictionary of Occupation on the career development of medical rep-resentatives in our country. Methods:The relationship of the Dictionary of Occupation and the career development of medical represent-atives was discussed, and then the influence of including the dictionary on medical representatives was analyzed. Results and Conclu-sion:The Dictionary of Occupation puts forward higher requests to the code of conduct, professional ability and professional ethics of medical representatives, which plays important roles in the career development of medical representatives. And as a professional stand-ard with clear definition and description, the Dictionary of Occupation also leads to the direction of sustainable and healthy development of medical representatives.

Objective To observe the clinical efficacy of moxibustion plus Cang Gui Tan Xue (dark turtle seeking hole) needling method in treating cervical spondylosis of nerve root type. Method Sixty patients with cervical spondylosis of nerve root type were randomized into a treatment group and a control group, 30 in each group. The treatment group was intervened by moxibustion plus Cang Gui Tan Xue needling method, and the control group was by simple needling method. The symptom and sign score was observed before and after 2-course treatment, and the clinical efficacy was compared. Result The total effective rate was 90.0%in the treatment group versus 83.3%in the control group, and the difference was statistically significant (P<0.05). After intervention, the symptom and sign scores were significantly changed in both groups (P<0.05). There was a significant difference in comparing the symptom and sign score between the two groups after intervention (P<0.05). Conclusion Moxibustion plus Cang Gui Tan Xue needling method is effective in treating cervical spondylosis of nerve root type.

Fecal microbiota transplantation(FMT)is a method to recover the gut microbiota and treat the intestinal or non-intestinal diseases through transplanting the fecal liquid from healthy population into patients' gastrointestinal tract.There are limited data about the FMT in children.The clinical practice needs professional workgroups,strict indications,normalized and precise procedures.

Using bioactive compounds 7-hydroxy flavone, salicylaldehyde, cinnamic acid and 4-amino-5- mercapto-1,2,4-triazoles as starting materials, three new types of flavone derivatives containing 1,2,4-triazole structure were synthesized via different step reactions. These new compounds were characterized by 1IHNMR, ESI-MS, IR and elemental analysis. Their scavenging effects on the superoxide radical (O2·-), hydroxyl radical (·OH), DPPH · radical and their total reduction activities were tested. The results showed that all of the compounds possessed some antioxidative activity at the concentration of 0.5 mg · mL(-1), but the scavenging ability of the target compounds was lower than that of the standard compound Vc.

Objective To evaluate the accuracy of stoke volume variation (SVV) determined using FloTrac/Vigileo and Picco-plus technologies in prone position for assessment of the blood volume in the patients undergoing spine surgery,Methods Forty-three ASA physical status Ⅰ-Ⅲ patients of both sexes,aged ＞ 18 yr,weighing 40-100 kg,scheduled for elective posterior approach to lumbar spinal fusion or scoliosis surgery were studied.After induction of anesthesia,a volume expansion was performed in supine and prone positions.Hydroxyethyl starch 130/0.4 sodium chloride injection 5 ml/kg was rapidly infused intravenously over 10 min to carry out the test for fluid responsiveness.Picco-plus and FloTrac/Vigileo systems were simultaneously applied in every subject to measure SVV (SVVP and SVVF).Positive fluid responsiveness was defined as the changing rate of stroke volume index ≥ 10％ as measured by using Piccoplus system.The patients were divided into response group (Rs group) and non-response group (NRs group) according to the changing rate of stroke volume index ≥ 10％ and ＜ 10％.The receiver operating characteristic (ROC) curve for SVV was plotted,and the diagnostic threshold,area under the ROC curve and 95％ confidence interval (CI) were calculated.Results Forty-one patients were included for analysis in this study.In supine position,the area under the ROC curve for SVV in predicting the fluid responsiveness was 0.740 (95％ CI:0.568-0.913),the diagnostic threshold was 12％,and the sensitivity and specificity in determining fluid responsiveness were 86％ and 54％,respectively,for SVVF,and the area under the ROC curve was 0.637 for SVVP.In prone position,the area under the ROC curve was 0.451 for SVVF,and 0.634 for SVVP.Compared with Rs group,the baseline value of SVVFwas significantly lower,and no significant change was found in the other hemodynamic parameters before volume expansion in supine position in NRs group.There was no significant difference in the hemodynamic parameters before volume expansion in prone position between the two groups.Conclusion SVV determined by using FloTrac/Vigileo and Picco-plus systems in prone position can not accurately assess the blood volume in the patients undergoing spine surgery.

Objective To study the protective effect and molecular mechanism of trypsin inhibitor on reperfusion injury after cerebral ischemia. Methods The model of ischemia for 1h and repufusion for 24h of rat cerebrum was set by ligating MCAO described by zea-longa. 24 male rats were divided randomly into the sham operation group, the control group and trypsin inhibitor group. The presence of neurological function deficit was measured by Zea-Longa method, and the immunohistochemical staining and TUNEL were used to detect p53 protein expression and cell apoptosis in the brain tissues respectively. Results The score of neurological function deficit was zero in the sham operation group, 2 8?1 0 in the control group and 1 3?0 7 in trypsin inhibitor group. There were 12 3?2 5 p53 immunostaining positive cells in the control group and 5 5?1 3 in trypsin inhibitor group. The number of apoptotic cells was zero in the sham operation group, 7 6?1 0 in the control group, and 3 5?0 9 in trypsin inhibitor group. There was a significant difference in all above observing indices between the control group and trypsin inhibitor group(P

Objective To discuss the application value of the treatment protocol of combining warm needling and atorvastatin in treating female cardiac syndrome X (CSX). Method Eighty female CSX patients were randomized into a control group and an observation group by the random number table, 40 cases in each group. The control group was intervened by atorvastatin in addition to the conventional treatment protocol, while the observation group was by warm needling plus atorvastatin in addition to the conventional treatment. The treatment efficacies and adverse events rates were compared between the two groups. Result There were no significant between-group differences in comparing the component and total scores of the Cardiac Anxiety Questionnaire (CAQ), NO, plasma endothelin (ET-1) and hypersensitive C-reactive protein (hs-CRP) levels before the intervention (P>0.05);after 8-week treatment, the component and total scores of CAQ, ET-1, and hs-CRP levels were significantly lower in the observation group than in the control group, and NO level was significantly higher in the observation group than in the control group (P<0.05). Meanwhile, at the end of the 8-week treatment, the total effective rate of the observation group was significantly higher than that of the control group (P<0.05). Besides, there was no significant difference in comparing the adverse events rate between the two groups during the 8-week treatment (P>0.05). Conclusion In the treatment of female CSX, warm needling plus atorvastatin can enhance the treatment efficacy.